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Microbial lysozyme microcapsule as well as preparation and application of microbial lysozyme microcapsule

A lysozyme and microcapsule technology, applied in the field of microbial preparations and aquaculture, can solve the problems of preparing microbial lysozyme microcapsules, etc., and achieve the effect of solving the problems of drug resistance and drug residues

Inactive Publication Date: 2013-04-03
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Up to now, there is no report on the preparation of microbial lysozyme microcapsules by using sodium alginate / chitosan as the wall material

Method used

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  • Microbial lysozyme microcapsule as well as preparation and application of microbial lysozyme microcapsule
  • Microbial lysozyme microcapsule as well as preparation and application of microbial lysozyme microcapsule
  • Microbial lysozyme microcapsule as well as preparation and application of microbial lysozyme microcapsule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 100ml 40g / L sodium alginate solution and 100ml 2mg / mL lysozyme solution (the activity of lysozyme is 5000U / mg) and mix evenly, and drop the above mixed solution into 200ml containing 20g / L calcium chloride and 50g / L chitosan (90% degree of deacetylation) and 5mL / L acetic acid aqueous solution, and stirred at a speed of 180rpm, the peristaltic pump dropping rate is 100 drops / min (about 4 ~ 6mL / min).

[0022] After about 40 minutes, the dropwise addition was completed, and the stirring was stopped. After solidification for 1 hour, the solid was washed twice with distilled water, and drained with gauze.

[0023] Pre-freeze at -20°C for 12 hours, freeze-dry at -25°C and 80pa for 24 hours. 2% calcium chloride and 0.5% chitosan (90% degree of deacetylation) in 0.5% (v / v) acetic acid aqueous solution with constant stirring, 10^6U / 4

[0024] The obtained lysozyme microcapsules use sodium alginate / chitosan as a wall material, and the content of lysozyme is 23000-27000U / g. ...

Embodiment 2

[0026] The above-mentioned lysozyme microcapsules that are made carry out heterogeneous gibel crucian carp culture test:

[0027] 1 Materials and methods

[0028] 1.1 Test fish and feed preparation

[0029] The experiment was carried out in the special aquaculture farm of Shanghai Ocean University from July to September 2011. The heterogeneous gibel crucian carp was selected in the same year, and it was cultured for 40 days with basic feed in the test pond to make it adapt to the test conditions and eliminate the differences caused by the nutrition and environment of the test fish. The experiment was divided into 11 groups with 4 repetitions in each group and 30 tails in each repetition (initial weight 28.11±2.14g). According to the nutritional requirements of heterogeneous gibel crucian carp, formulate feed formula and make basic feed. Add lysozyme product and its coating product respectively in basic feed, wherein lysozyme product is the microbial lysozyme crystal powder ...

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Abstract

The invention relates to the fields of microorganism preparations and aquaculture, and discloses a lysozyme microcapsule. The microbial lysozyme microcapsule is prepared by the following steps of: (1) dropping a mixing solution of sodium alginate and lysozyme into an acetic acid solution containing chitosan and calcium chloride, and stirring; (2) after dropping, curing for 0.5-4hours, and taking and washing a solid to obtain lysozyme / sodium alginate / chitosan beads; and (3) pre-freezing the lysozyme / sodium alginate / chitosan beads, and freeze-drying. The microcapsule disclosed by the invention is used for preparing fish feed, and is capable of reserving comparatively high activity of the microbial lysozyme after being extruded at the high temperature in the feed processing; in the process of conserving at normal temperature, the stability of the microbial lysozyme can be kept for more than half an year; and in feeding and breeding, the microbial lysozyme can bear the strong acid and digesting environment in the stomach of the aquatic animal, release in the intestinal tract and develop an effective growth-promoting healthcare effect.

Description

technical field [0001] The invention relates to the field of microbial preparations and aquaculture, in particular to a microbial lysozyme microcapsule and its preparation method and application. Background technique [0002] Nowadays, the phenomenon of aquatic food being contaminated by drugs and endangering human health is becoming more and more serious. Many countries have banned or restricted the application of antibiotics in feed. Therefore, it is an urgent need for the aquaculture industry of various countries to find natural and safe alternatives to antibiotics. solved problem. [0003] Microbial lysozyme is an enzyme secreted by microorganisms that can dissolve bacterial cell walls. Because it has higher productivity and wider antibacterial spectrum than egg white lysozyme, it has been used in food, medicine, enzyme engineering and feed in recent years. It has attracted much attention in the industry, so it is of great significance to use it as an antibiotic substit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/165A23K1/18
Inventor 李晓晖费国琴华雪铭邢思华宁喜斌
Owner SHANGHAI OCEAN UNIV
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