Canine distemper virus and canine coronavirus duplex PCR detection kit and detection method

Abstract

The invention discloses a canine distemper virus and canine coronavirus duplex PCR detection kit and a duplex PCR method for rapid identification and detection of canine distemper virus and canine coronavirus. According to the genome sequences of the canine distemper virus and canine coronavirus, a pair of PCR primers are designed respectively to amplify corresponding specific fragments and realize detection and identification of canine distemper and canine coronavirus. The invention utilizes the duplex PCR technology to conduct single tube synchronous detection of canine distemper virus and canine coronavirus, reduces the detection cost and workload of canine distemper virus and canine coronavirus, and realizes identification and detection of canine distemper virus and canine coronavirus. The invention establishes a duplex PCR method that strong specificity and high sensitivity, saves time and labor, and is easy to observe the result, and has the advantages of simple operation, strong specificity, high sensitivity and repeatability, and no complicated aftertreatment.

Description

technical field [0001] The invention relates to a kit for canine distemper virus and canine coronavirus, which belongs to the field of animal epidemic molecular biology detection methods and detection reagents, in particular to a double PCR detection kit for simultaneous detection of canine distemper virus and canine coronavirus and A biological detection method for simultaneous dual PCR detection of canine distemper virus and canine coronavirus using a kit. Background technique [0002] Canine distemper virus (CDV) and canine coronavirus (CCV) are two sources of viral infectious diseases that seriously endanger the dog industry, economic animal breeding and wildlife protection (Cheng Yuening et al., 2013; Sun Yuanyuan et al., 2009). The mixed infection of canine distemper and canine coronavirus often occurs clinically, complicating the clinical symptoms and making the diagnosis of the disease difficult (Soma et al., 2011). At present, the clinical diagnosis of CDV and CCV...

AI Technical Summary

Problems solved by technology

At present, the clinical diagnosis of CDV and CCV mainly relies on traditional etiological and serological diagnostic methods. Virus isolation and culture and electron microscope observation have strong specificity, but they are time-consuming, require professional equipment, and are not suitable for rapid diagnosis and grass-roots application requirements. (Li Tiansong et al., 2012; Cha et al., 2012)

The serological detection method is relatively simple, but after the body is infected with the virus, it takes a certain period of incubation before viremia occurs, which is not conducive to early diagnosis

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