The invention discloses an
adjuvant for improving the effect of a porcine reproductive and respiratory syndrome (PRRSV)
inactivated vaccine, a preparation method thereof and application thereof and relates to the preparation method and steps of Hemagglutinating
virus of Japan envelope (HVJ-E) and application of a medicament for improving the effect of the PRRSV
inactivated vaccine. The preparation method of
adjuvant comprises the following steps of: breeding Hemagglutinating
virus of Japan (HVJ) by using an SPF chick
embryo, and then concentrating, purifying, counting and inactivating the HVJ to prepare the Hemagglutinating
virus of Japan envelope (HVJ-E); immunizing PRRSV negative pigs with the HVJ-E and the PRRSV
inactivated vaccine together, and setting a PBS contrast group and a PRRSV inactivated vaccine group at the same time, wherein compared with the contrast group, the HVJ-E and PRRSV inactivated vaccine group can obviously improve the
response level of hunoral
immunity and
cellular immunity of the pigs; and after immunizing, expelling
toxin by using a PRRSV virulent strain (JXA1), wherein the result shows that the clinical manifestations and the
weight increasing of the HVJ-E and PRRSV inactivated vaccine group are better than those of the contrast group, and the generated viremiavirusemia rate,
toxin expelling rate and virus distribution rate are obviously reduced when compared with the contrast group. The
adjuvant of the invention has the advantages of convenient production and low cost and is suitable for large-scale production.