The invention discloses a polypeptide sequence specifically bind to polystyrene and a method for improving polypeptide and protein antigen for surface of a polystyrene material. By adopting a phage display random peptide library screening, the polypeptide PB-TUP which is subjected to affinity binding with polystyrene is obtained, an amino acid sequence is Val-His-Trp-Asp-Phe-Arg-Gln-Trp-Trp-Gln-Pro-Ser; and a coding nucleotide sequence is GTG-CAT-TGG-GAT-TTT-CGG-CAG-TGG-TGG-CAG-CCT-TCT. The method has the following advantages that 1) then affinity peptide PB-TUP has strong affinity binding capability on a polystyrene carrier; 2) the affinity peptide PB-TUP and polypeptide or protein antigen are fusion, so that the immobilized effect of antigen can be effectively improved; 3) the affinity peptide PB-TUP can guide the fusion antigen to immobilize on the surface of polystyrene by an unified and ordered mode, the space conformation of the antigen is kept, and the inactivation of antigen active sites due to shielding can be avoided; and 4) the method can be used for diagnosis detection technologies, such as enzyme linked immunosorbent assay, biology, cytological detection, and biosensors and micro-array chips which take the polystyrene as a fixed carrier, and improves detection sensitivity.