Epitope of spring viraemia of carp

Abstract

The invention relates to an antigenic epitope of spring viraemia of carp and belongs to the technical field of biology. According to the invention, by virtue of phage display technique, a positive clone is screened out by the use of spring viraemia of carp virus (SVCV) monoclonal antibody, a conserved sequence binding to the SVCV monoclonal antibody is found out, a clinical diagnosis reagent is prepared by the screened antigenic epitope, and spring viraemia of carp is prevented and treated by a small molecular antigen peptide as a vaccine. The gene sequence of the invention is SEQ ID NO:1. The phage display technique is utilized to screen the positive clone by SVCV monoclonal antibody and find out the conserved sequence which can bind to the monoclonal antibody with the ability of SVCV activity neutralization in a random peptide library. The site of the antigenic determinant is determined, and the relationship of its sequence, structure and function is analyzed. The study of inhibition effect to SVCV generated from the competitive binding of small peptide to SVCV receptor will have a huge application value for taking small molecular antigen peptide as vaccine in the future.

Description

technical field [0001] The invention belongs to the field of biotechnology. Background technique [0002] my country is a big aquaculture country, the total amount of aquaculture is the first in the world, and the export volume of aquatic products is the first in the world. Carp spring viremia (SVC) is one of the most important diseases of fish, characterized by acute onset and high mortality. Carp spring viremia virus has a wide range of hosts and can infect the four major carps and other carps, including carp and koi, bighead carp, grass carp, silver carp, black crucian carp, crucian carp, tench mandarin carp and catfish, etc. , carp is the main and most susceptible host. In recent years, the disease has occurred in most parts of our country, and has become the main disease of freshwater cultured fish in our country. Carp spring viremia is a kind of infectious disease of aquatic animals, and it is the first type of quarantine object for fish port quarantine. [0003] A...

AI Technical Summary

Problems solved by technology

Although PCR technology has the advantage of high sensitivity, its disadvantages are:

[0004]1. False positives often appear in the result judgment;

[0005]2. Professionals and expensive instruments and reagents are required;

[0006]3. Does not meet the detection method recommended by the International Office of Epizootics (OIE)

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