125results about How to "Strong replicability" patented technology

Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and / or cells (e.g., Vero and / or MDCK) and further provides influenza viruses with enhanced replication characteristics. A method of producing a cold adapted (ca) influenza virus that replicates efficiently at, e.g., 25° C. (and immunogenic compositions comprising the same) is also provided.

Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and / or cells (e.g., Vero and / or MDCK) and further provides influenza viruses with enhanced replication characteristics. In addition, the present invention includes an improved method of rescue, wherein animal cells (e.g., SF Vero cells) are electroporated with plasmids and vectors of the invention.

The invention relates to an on-line shopping distribution station management system. The system comprises a central monitoring system and a distribution system; the central monitoring system consists of a service program running on a server and a central database, and can monitor the running state of the management system and the goods distribution condition in real time; the distribution system comprises a distribution station and an authentication machine arranged at the distribution station; and the authentication machine has identification and data transmission functions. The system is convenient, fast and quick for taking goods; idle resources of the conventional sites of community merchants or properties are fully integrated, and the problem of high cost in last mile distribution in the on-line shopping logistic distribution process is effectively solved; and the system becomes a logistic distribution system with high reproducibility, high portability and quick deployment.

Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and / or cells (e.g., Vero and / or MDCK) and further provides influenza viruses with enhanced replication characteristics.

The invention discloses a recombinant low-virulent vaccine strain of chicken infectious bursal disease viruses (IBDV) and application thereof. In the invention, a major protective antigen gene VP2 of an epidemic superhigh virulent strain is cloned, the nucleotide of the gene VP2 is modified by mutation and then used for replacing a corresponding segment of a Gt genome of a low-virulent strain of the IBDV, so that the infectious clone of a recombinant genome of the IBDV is constructed, and the recombinant low-virulent vaccine strain is saved and identified by using an IBDV reverse genetic operation system. The microbial collection number of the vaccine strain is CGMCC No.3749. The recombinant low-virulent vaccine strain of the invention has high replicability, genetic stability and safety. The immune effect of the low-virulent vaccine strain of the invention is as good as that of the medium-virulent vaccine strain, but is superior to that of the low-virulent vaccine strain. The biological safety of the low-virulent vaccine strain of the invention is superior to that of the medium-virulent vaccine strain. As the vaccine strain, the recombinant low-virulent vaccine strain of the invention has the characteristics of high efficiency and low toxicity, is a good candidate vaccine strain and can be used for controlling chicken infectious bursal disease.

The invention relates to an environment-friendly kitchen waste disposer and a waste disposal method by using the waste disposer. The environment-friendly kitchen waste disposer is embedded underground and comprises a waste accommodating layer, a water system heating layer, a heat complementing layer and a heat insulating layer, wherein a smashing device for smashing wastes and biodegradation complex bacteria and / or biodegradation enzymes for degrading the wastes are arranged in the waste accommodating layer; and smashed and degraded fluid is discharged into a septic tank or a blowdown pipe through a drainage outlet. The waste disposal method comprises the following steps: (1) placing the environment-friendly kitchen waste disposer underground; (2) smashing the kitchen wastes by using the smashing device; and (3) fermenting the biodegradation complex bacteria and / or biodegradation enzymes and the wastes for degradation; and 4, discharging or recycling part of or all of fermented humus to the septic tank. The environment-friendly kitchen waste disposer does not occupy area, does not exhaust odor to the atmosphere, and is free of noises and low in energy consumption and suitable for places, such as canteens, communities, hotels, dining mechanisms and the like where the kitchen wastes are centralized.

A ring gear includes teeth (10) and tooth gaps (12), in which the teeth (10) can be brought vie tooth flanks (14) into engagement with a corresponding pinion. The tooth flanks (14), after a final engagement point (16) of the pinion, from the tooth head (18) toward the tooth base (20), compared to standard tooth flanks (22), are made to approximate a trochoid (24, 26), described by the pinion and projected into a normal section.

Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and / or cells (e.g., Vero and / or MDCK) and further provides influenza viruses with enhanced replication characteristics. In addition, the present invention includes an improved method of rescue, wherein animal cells (e.g., SF Vero cells) are electroporated with plasmids and vectors of the invention.

The application discloses a production method of a shell for an electronic device, the shell and the electronic device. The production method of the shell comprises the following steps of providing atransparent sheet material, wherein the sheet material comprises oppositely arranged apparent surface and inner side surface; forming a texture layer on the inner side surface of the sheet material; and carrying out dyeing treatment on the texture layer. According to the production method of the shell for the electronic device, which is provided by the embodiment of the application, through forming the texture layer on the inner side surface of the sheet material and carrying out the dyeing treatment on the texture layer, the shell can be enabled to have a color; further, the color of the shell can be enabled to be uniform, natural, high in replicability and further more transparent; and the overall thickness of the shell can be decreased.

According to one embodiment, a method for providing an enhanced directory service includes providing a supplemental layer between a user and a reference layer, the supplemental layer providing the user with any directory functionality provided by the reference layer as well as additional directory functionality.

The invention belongs to the field of macromolecule composites and relates to a PBT material with high flow, fine finish and non-basecoat and the preparation method thereof. The PBT material of the invention comprises the following constituents: 88 to 98 parts of PBT; 1 to 5 parts of nucleation a agent; 0.5 to 4 parts of a lubricant; 0.1 to 3 parts of a antioxidant; 0.05 to 0.1 parts of a meso-material. The preparation method comprises the steps of, putting all raw materials into a twin-screw machine after 10 to 15 min of mixing in a high speed mixer; performing melt extrusion and granulation. The twin-screw machine is divided into 10 zones from a feeding inlet to a head. The PBT material of the invention has a detailed uniform micro spherulite structure and a good surface finish. An injection molding blank obtained by the PBT material can be directly aluminized in vacuum and can solve the problem of severe micromolecule migration to the aluminum layer of non-basecoat PBT material when used in high temperature. The material has strong mold reproduction capability and the obtained products are more delightful.

The invention discloses a method and equipment for database backup. Through the technical scheme of the invention, storage equipment in a low-bandwidth network environment can be used for data disaster recovery so that bandwidth is widened by using network concurrency in a copying process, the integrity of copy is promoted, data required to be usually used and modified are integrated onto the network storage equipment with good network status and the data not required to be usually used and modified are stored onto the network storage equipment with relatively poor network status. The method and the equipment guarantee the service performance after a disaster recovery center substitutes when a production center breaks down while the copy performance is improved.

The invention relates to an indirect measuring system for electricity consumption of an end device of an air conditioning system of a public building. The system comprises a public building information acquiring module for acquiring building type and distribution branch information, an electricity energy sub-item measuring module for acquiring the electricity consumption data of each sub-item, a end device energy consumption decomposing module for receiving the building type, distribution branch information and sub-item electricity consumption data as well as decomposing the air conditioning system end device mixed in the electricity consumption sub-item of a lighting socket and / or a power electricity consumption sub-item so as to obtain the electricity consumption dismounting result of the air conditioning system end device, and an energy consumption discomposing result evaluation module for receiving the energy consumption decomposing result of the air conditioning system end device and evaluating the advantages and disadvantages of the energy consumption decomposing result of the air conditioning system end system according to the preset evaluation indicators. Compared with the prior art, the system has the advantages that the energy consumption of each device can be clear; the automation degree is high; the practicability is high; the use is convenient.

According to one embodiment of the invention a method of updating schema in a directory system having a reference layer adapted to provide directory functionality and an intermediate layer adapted to provide supplemental directory functionality is provided. The intermediate layer is provided between the reference layer and the client is provided. The method includes determining that a new attribute type is not defined in a schema associated with the system, and in response, registering the new attribute type in the schema.

The invention relates to a method and a system for automatically identifying suspected familial defects of power transmission and transformation equipment. The method comprises the following steps of:collecting data of power transmission and transformation equipment, performing text analysis on defect information of the power transmission and transformation equipment to obtain a characteristic word frequency matrix of equipment defect information; based on the characteristic word frequency matrix of equipment defect information, calculating the correlation between word frequency vector matrices and the similarity between the defect information of different pieces of power transmission and transformation equipment; and based on the similarity of defect information of different pieces of power transmission and transformation equipment, clustering the defect information of the power transmission and transformation equipment to identify equipment with the suspected familial defects. The invention clusters the defect information of the power transmission and transformation equipment and automatically identifies the equipment with the suspected family defects according to a grouping statistical result, thereby greatly reducing the cost of manpower, time and the like.

The invention discloses an oral vaccine strain for recombined rabies virus. The oral vaccine strain adopts an SAD-B19 as a maternal strain; aspartic acid at the 194th position on a G protein of the maternal strain is mutated into serine, arginine at the 333rd position of the G protein is mutated into glutamic acid, and a dog GM-CSF gene is inserted between a G gene and an L gene of a gene group of the maternal strain. The invention also discloses a preparation method of the oral vaccine strain for the recombined rabies virus. The oral vaccine strain disclosed by the invention has good reproducibility and safety; the immunogenicity is enhanced by insertion of the dog GM-CSF gene; after oral immunization, a high-level neutralizing antibody can be induced, and the recombined rabies virus is obviously superior to LBNSE virus (a vaccine strain for immunization of wild animals in Europe); the recombined rabies virus can be used as a candidate strain of an oral vaccine for rabies virus.

The invention relates to a fracture three-dimensional size-controllable fracture network-matrix double-medium model and a preparation method thereof. The model is formed by combining different rock core segments; each of the rock core segments is formed by combining different rock core blocks formed by axially cutting along the rock core segment; a gap is formed among the different rock core blocks, a gap is also formed among the different rock core segments, and the gap among the different rock core blocks and the gap among the different rock core segments form a model fracture. Rock cores are cut into a certain quantity of rock core segments, the rock core segments are cut into the rock core blocks, and the rock core blocks and the rock core segments are sequentially spliced to be combined into the model. The fracture width of the fracture network-matrix double-medium model provided by the invention can be effectively controlled, the model shape can be flexibly adjusted, the manufacture is simple, and the model repeatability is strong.

The invention discloses an antagonistic drug against replication of a porcine reproductive and respiratory syndrome virus (PRRSV) and application of the antagonistic drug. A virus infection test is adopted, and Xanthohumol (Xn), the antagonistic drug of the PRRSV, is discovered for the first time from a natural medicine library with 386 plant sources. The drug can effectively inhibit PRRSV replication in both Marc-145 and PAM cells, five xanthohumol derivatives with different molecular structures are artificially synthesized, and the viral infection test further finds that the inhibition effect to virus replication by drug derivative Xn-4 is highest in vitro; and test results of artificial infection and drug therapy in piglets show that the Xn-4 can effectively inhibit PRRSV viremia, relieves clinical symptoms of infected pigs, and significantly reduces pulmonary inflammation and pathological damage. Combined with the results, it is proved that the antagonistic drug has important preventive and therapeutic effects to a porcine reproductive and respiratory syndrome, and has important application prospects.

The invention discloses an adenovirus bivalent vaccine. The vaccine comprises a replication-deficient human adenovirus type 4 and a replication-deficient human adenovirus type 7. E1 and E3 genes of the replication-deficient human adenovirus type 4 and the replication-deficient human adenovirus type 7 are deleted, and partial coding frames of the E4 gene are replaced by corresponding coding framesof E4 gene of human adenovirus type 5. The vaccine can effectively stimulate an organism to generate a humoral immune response and a cellular immune response, generate specific neutralizing antibodieswith high titer, and is used for preventing infection of pathogens.

An injection mold composed of a movable mold and a fixed mold. The movable mold has bases, a heat insulating layer and a surface processed layer, and the fixed mold has a base. A heat insulator is provided on the inner circumferential surface of the base of the movable mold at a part forming a wall of a cavity. The heat insulating layer is in the rear of the surface processed layer, and therefore, the transfer accuracy of a fine configuration of the surface processed layer is improved. Additionally, since the heat insulator is provided adjacent to the fine configuration, heat radiation from resin is inhibited, and the transfer accuracy of the fine configuration is further improved.

The invention belongs to the technical field of biology, and particularly relates to a corynebacterium and colibacillus double expression vector with high copy capability. The nucleotide sequence of an expression plasmid is shown as SEQ ID NO:9. Through artificial mutagenesis on a nucleotide C in a 1786-th site in a corynebacterium replicon regulation position of a pXMJ19 carrier, a corynebacterium / colibacillus dual-purpose vector pXMJ19C1786T plasmid with the copy capability being about 12.5 times higher than that of a pXMJ19 plasmid is successfully obtained. The copy number of the pXMJ19 plasmid in the corynebacterium is about 20; and the copy number of the pXMJ19C1786T plasmid in the corynebacterium is 251. Meanwhile, the invention also discloses a building method of the expression plasmid.

The invention discloses a telomerase composition, a preparation method thereof and application thereof in an anti-aging and anti-inverse-growth cosmetic. The telomerase composition is obtained by adding a broccoli telomerase extract, a Chinese pine leaf telomerase extract, a ginkgo leaf telomerase extract and a telomerase activator. The invention also provides an anti-aging and anti-inverse-growthcosmetic added with the telomerase composition and a preparation method thereof. Compared with the prior art, the prepared telomerase composition and anti-aging and anti-inverse-growth cosmetic haveexcellent anti-aging and anti-inverse-growth properties.

The invention discloses a recombinant A subgroup avian leukosis virus being able to express an ALV-J envelope protein, and a construction method and a use of the virus. The virus adopts ALV-A infectious clone as a skeleton, the envelope protein of the ALV-A infectious clone is replaced by the ALV-J envelope protein, and the 3' end of the envelope protein carries luciferrase report gene, so the infectious clone of the recombinant ALV-A virus being able to express the ALV-J envelope protein is constructed. Experiments prove that the recombinant virus obtained through infectious clone rescuing has high replication ability, the tilter of the virus reaches 10<5.21> TCID50 / ml, and is 125 times that of an ALV-J original strain, and the luciferrase report gene is carried out in order to realize easy quantification. The recombinant virus can infect 293T cells for expressing chNHE1, and can be detected 8h after infection at the earliest, so the recombinant virus has high sensitivity. The a recombinant A subgroup avian leukosis virus solves the problems of low virus titer, low replication ability, and adverseness of a small amount of the virus in the early stage of virus infection to detection of traditional ALV-J viruses, and is of great significance to carry out researches related with viruses and virus receptors and anti-ALV-J antibody detection.

A positive resist composition that is capable of forming fine resist patterns, capable of appropriately controlling the angle of taper configuration of resist patterns and capable of forming resist patterns excelling in focal depth breadth. This positive resist composition consists of a chemical amplification type positive resist composition and realizes a transmission of 248 nm wavelength light through 0.3 mum thick resist film of 20 to 75%.

The embodiment of the invention provides a log processing method and device based on a docker container, and the method comprises the steps: deploying a filebeat at each host machine needing to collect log data through employing a docker container, and enabling the filebeat to be used for log data collection; generating kafka and zookeeper cluster configuration files in a host machine, and performing deployment by adopting docker container deployment so as to perform log data transmission; generating an elk cluster configuration file in a host machine, and adopting docker container deploymentfor log data processing and display; and generating an nginx configuration file in the host machine, and performing deployment by adopting the docker container for log data access control. According to the embodiment of the invention, standalone cluster deployment based on the docker container is realized, so that the investment and expenditure of physical machines are saved, and the whole processing process becomes simple and efficient.

The invention relates to the display and demonstration industry, particularly to a medium-free transparent imaging showcase. A showcase is arranged, the upper end of the showcase is provided with a projector, a medium-free transparent imaging system is installed in the showcase, information contents of items on display in the showcase can be effectively propagated, and the method of digital multimedia is used to provide self-service exhibition guide for visitors, thereby saving human cost for interpretation.

A method for the cultivation of human tumor suppressor gene green fluorescent protein carrier, design primers on GENBANK with reference to human anti-cancer gene sequence, collect peripheral blood of early lung cancer patients to extract total RNA, and use reverse transcription polymerase chain reaction (RT-PCR) Amplify the P53 fragment, clone the gene fragment directionally onto the pMD18-T vector, construct the pMD18-P53 recombinant plasmid, identify the recombinant plasmid by PCR amplification, enzyme digestion and sequencing, and digest the pMD18-P53 Identify and extract the directional clone of the P53 target gene into the pEGFP-N1 eukaryotic expression vector to construct the pEGFP-N1-P53 expression vector. The beneficial effects of the present invention are: using the eukaryotic expression vector pEGFP-N1, which is safe, stable, strong in replication, high in expression rate, and easy to detect, as the transport carrier of human anti-cancer genes, thereby constructing a safe, stable, easy-to-detect, safe and reliable The eukaryotic expression vector pEGFP-N1-P53 is used for the prevention and treatment of cancer.

The invention discloses a technology of utilizing an Apriori algorithm to carry out optimization parallel database storage efficiency in a database replication environment. Sets of tables with high relevance, namely the table sets, are found through carrying out Apriori relevance analysis on transaction information in the database replication environment. Transactions of modifying the tables belonging to the same table set are assigned, according to table names in the table set, to the same database connection for execution, transactions belonging to the different table sets are assigned to different connections for execution, thus interference between the connections is reduced to the greatest extent, and performance of parallel multi-connection processing is fully utilized.