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Preparation method of telomerase composition and application of telomerase composition in anti-aging and anti-inverse-growth cosmetic

A technology of telomerase and composition, applied in the direction of cosmetics, cosmetic preparations, dressing preparations, etc., to achieve the effect of restoring skin elasticity, reducing wrinkles and reducing spots

Active Publication Date: 2018-03-27
上海联衡生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few reports to prepare anti-aging and anti-growth cosmetics by adding telomerase composition

Method used

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  • Preparation method of telomerase composition and application of telomerase composition in anti-aging and anti-inverse-growth cosmetic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Preparation of broccoli telomerase extract:

[0063] Take 5g of fresh broccoli buds and put them into a mortar, add 5mL of liquid nitrogen and grind them into powder, add 20mL of extract and continue grinding for 10min;

[0064] Transfer the ground mixture into a 50mL centrifuge tube, centrifuge at 4°C and 15000rpm / min for 15min, divide the supernatant into 2mL EP tubes, and add 1mL supernatant to each EP tube;

[0065] Add 0.2g PEG 6000 to each EP tube, shake in a low-temperature shaker at 5°C for 1h, then centrifuge at 4°C and 15,000rpm / min for 2min, remove the supernatant, and store the lower layer at -80°C Preserve to obtain broccoli telomerase extract.

[0066] The extract is 1mL 1mol / L pH 7.5 Tris-HCl buffer solution, 4mL 25mmol / L magnesium chloride, 2mL1mol / L potassium chloride, 4mL 100mmol / L ethylene glycol bis(2-aminoethyl ether)tetraacetic acid, 166 μL 12 mmol / L phenylmethylsulfonyl fluoride, 4 mL 7.5 wt% polyvinylpyrrolidone, 4 mL 50 wt% glycerol, 200 μL 10...

Embodiment 2

[0078] Preparation of Pinellia palmata extract:

[0079] The Pinellia palmata medicinal material was crushed in a CJ-20 wall-breaking grinder of Jinan Changji Machinery Equipment Co., Ltd. at a speed of 900 rpm / min for 30 minutes, and passed through a 1000-mesh sieve to obtain the Pinellia palmata medicinal material powder;

[0080] Take 3g of Pinellia palmatum medicinal material powder and place it in a 250mL conical flask with a stopper, add 90mL of methanol and 10mL of ammonia water, and seal it tightly; in a constant temperature water bath at 25°C, sonicate for 60min, suction filter and wash twice with methanol, each washing with 20mL of methanol, Collect the resulting filtrate;

[0081] The above-mentioned filtrate was evaporated in a rotary evaporator at 50°C until it was in the form of an extract, and then freeze-dried in a freeze dryer to obtain Pinellia palmata extract.

[0082] Preparation of astragalus extract:

[0083] The Radix Astragali medicinal material was p...

Embodiment 3

[0087] A preparation method of telomerase composition:

[0088] Shake 45 parts by weight of telomerase, 7 parts by weight of telomerase activator, 2 parts by weight of carnosine, 1 part by weight of chitin, 2 parts by weight of melatonin and 50 parts by weight of deionized water in a low-temperature shaker at 5°C Mix for 20 minutes to obtain the telomerase composition.

[0089] Described telomerase is by mass ratio 1:1 by the broccoli telomerase extract of embodiment 1, the pine leaf telomerase extract of embodiment 1, the ginkgo leaf telomerase extract of embodiment 1: 1 blended.

[0090] The telomerase activator is prepared by mixing Pinellia palmata extract in Example 2, Astragalus extract in Example 2, and astaxanthin in a mass ratio of 1:1:1.

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Abstract

The invention discloses a telomerase composition, a preparation method thereof and application thereof in an anti-aging and anti-inverse-growth cosmetic. The telomerase composition is obtained by adding a broccoli telomerase extract, a Chinese pine leaf telomerase extract, a ginkgo leaf telomerase extract and a telomerase activator. The invention also provides an anti-aging and anti-inverse-growthcosmetic added with the telomerase composition and a preparation method thereof. Compared with the prior art, the prepared telomerase composition and anti-aging and anti-inverse-growth cosmetic haveexcellent anti-aging and anti-inverse-growth properties.

Description

technical field [0001] The invention relates to the technical field of telomerase composition, in particular to the application of the telomere composition in anti-aging and anti-growth cosmetics. Background technique [0002] The 2009 Nobel Prize-winning research revealed the root cause of aging. A genetic structure invisible to the naked eye called telomeres is the answer to the mystery of aging. Telomeres are composed of repetitive DNA sequences, similar to the protective caps on the tiny ends of shoelaces, which form a cap at the end of chromosomes and protect our DNA. When humans are born, telomeres are long. Over time, telomeres begin to shorten. Telomeres shorten as cells divide, starting the journey of cellular aging. When telomeres become too short, cells are unable to perform their functions. Begins to self-destruct and eventually dies. Scientists call this apoptosis (apoptosis). Apoptosis is one thing, but when a cell shuts down, it expels free radicals and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/66A61K8/64A61K8/73A61K8/49A61K8/9789A61K8/9767A61K8/9771A61K8/9794A61K8/35A61Q19/08A61Q19/02
CPCA61K8/35A61K8/492A61K8/64A61K8/66A61K8/736A61K8/97A61K2800/5922A61K2800/82A61K2800/84A61Q19/02A61Q19/08
Inventor 秦松辉
Owner 上海联衡生物科技有限公司
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