Oral vaccine strain for recombined rabies virus and preparation method thereof

Abstract

The invention discloses an oral vaccine strain for recombined rabies virus. The oral vaccine strain adopts an SAD-B19 as a maternal strain; aspartic acid at the 194th position on a G protein of the maternal strain is mutated into serine, arginine at the 333rd position of the G protein is mutated into glutamic acid, and a dog GM-CSF gene is inserted between a G gene and an L gene of a gene group of the maternal strain. The invention also discloses a preparation method of the oral vaccine strain for the recombined rabies virus. The oral vaccine strain disclosed by the invention has good reproducibility and safety; the immunogenicity is enhanced by insertion of the dog GM-CSF gene; after oral immunization, a high-level neutralizing antibody can be induced, and the recombined rabies virus is obviously superior to LBNSE virus (a vaccine strain for immunization of wild animals in Europe); the recombined rabies virus can be used as a candidate strain of an oral vaccine for rabies virus.

Description

technical field [0001] The invention belongs to the technical field of animal vaccines, and in particular relates to a recombinant rabies virus oral vaccine strain and a preparation method thereof. Background technique [0002] Rabies is one of the oldest infectious diseases and is a zoonotic infectious disease caused by Rabies virus (RV). Rabies is by far the acute infectious disease with the highest human fatality rate. Once it occurs, the fatality rate is almost 100%. According to the report of the World Health Organization (WHO), about 55,000 people die from rabies every year in the world, most of which are distributed in Africa and Asia. In recent years, about 3,000 people died of rabies every year in my country on average, most of them were bitten or infected by animals. Vaccine immunization is the most effective way to prevent and control rabies. The currently widely used rabies vaccines are mainly inactivated vaccines such as primary cell culture vaccines and passa...

AI Technical Summary

Problems solved by technology

VR-G is a recombinant strain of poxvirus expressing rabies G protein. Although VR-G has helped many European and American countries prevent the spread of rabies in wild animals such as raccoons and foxes, some studies have shown that exposure of VR-G to humans can cause Severe skin inflammatory response and systemic poxvirus infection (Mempel M, Isa G, Klugbauer N, Meyer H, Wildi G, et al. Laboratory acquired infection with recombinant vaccinia virus containing an immunomodulating construct. J Invest Dermatol. 2003, 120 : 356-358. Rupprecht CE, Blass L, Smith K, Orciari LA, Niezgoda M, et al. Human infection due to recombinant vaccine-rabies glycoprotein virus. N Engl J Med. 2001, 345: 582-586. Centers for Disease C, Prevention. Human vaccine infection after contact with a raccoon rabies vaccine bait-Pennsylvania, 2009. MMWR Morb Mortal Wkly Rep. 2009, 58:1204-1207.)

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