Synthetic polypeptide composition for novel coronavirus immunodetection and application thereof

A technique for synthesizing polypeptides and coronaviruses, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as false negatives, misdiagnoses and missed diagnoses, and false positives in nucleic acid detection, and achieve the effects of low cost, easy access, and easy promotion and use

Active Publication Date: 2020-06-26
BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Due to methodological limitations, false positives and false negative...

Method used

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  • Synthetic polypeptide composition for novel coronavirus immunodetection and application thereof
  • Synthetic polypeptide composition for novel coronavirus immunodetection and application thereof
  • Synthetic polypeptide composition for novel coronavirus immunodetection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of magnetic particles-anti-FITC antibody includes the following steps. Take 200mL of 0.2M hydroxyethylpiperazine ethylsulfuric acid (HEPES) buffer solution, add 60mg of magnetic particles with amino or carboxyl groups on the surface, stir at room temperature for 30min, and then add 21mg of anti-FITC antibody. FITC antibody, then add EDC, its concentration is 10mg / mL, after 1h reaction in the dark at room temperature, wash 3 times with 0.01M PBS buffer solution, finally use 0.01M PBS to fix the solution to 1L; The magnetic particle is The surface is coated with ferric iron tetroxide with amino or carboxyl active groups, and the particle size is 1-2 μm.

[0030] The preparation of the FITC-labeled novel coronavirus antigen comprises the following steps,

[0031] 1) Mix FITC and novel coronavirus synthetic peptide at a molar ratio of 1:1, and react at 37°C for 12 hours in the dark.

[0032] 2) Dialyze the labeling solution completed in the above step 1) ...

Embodiment 2

[0104] The preparation of magnetic particles-anti-FITC antibody includes the following steps. Take 100mL of 0.1M morpholineethanesulfonic acid (MES) buffer solution, add 20mg of magnetic particles with amino or carboxyl groups on the surface, stir at room temperature for 40min, and then add 7mg of anti-FITC antibody. Then add EDC at a concentration of 8mg / mL, react at 2-8°C for 1h, wash with 0.01M PBS buffer for 3 times, and finally dissolve it with 0.01MPBS to 1L; the magnetic particles are surface-coated tapes Ferric iron tetroxide with amino or carboxyl active groups, particle size 1-2 μm.

[0105] The preparation of the FITC-labeled novel coronavirus antigen comprises the following steps,

[0106] 1) Mix FITC and novel coronavirus synthetic peptide at a molar ratio of 1:1, and react at 37°C for 12 hours in the dark.

[0107] 2) Dialyze the labeling solution completed in the above step 1) with 0.01M PBS at 2-8°C for 24 hours, add an equal volume of glycerol, and store at...

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Abstract

The invention provides a synthetic polypeptide composition for novel coronavirus immunodetection. The synthetic polypeptide composition contains four synthetic polypeptides, the synthetic polypeptidedisclosed by the invention can react with an antibody IgG/IgM of a novel coronavirus (2019-nCoV), does not react with other pulmonary infectious coronaviruses and the like, and does not react with serum of a normal person; the synthetic polypeptide disclosed by the invention is used for detecting a novel coronavirus (2019-nCoV) IgM/IgG antibody in serum, the sensitivity can reach 90% or above, thespecificity can reach 99% or above, and the polypeptide can be obtained through chemical synthesis, does not need to be recombined through a biotechnology, and is lower in cost, easier to obtain andconvenient to apply and popularize.

Description

technical field [0001] The invention belongs to the technical field of immunoassay and detection, and in particular relates to a synthetic polypeptide composition and application for immunoassay of novel coronavirus. Background technique [0002] Novel coronavirus (SARS-CoV-2) is a new strain of coronavirus discovered in humans in 2019. Coronavirus (CoV) belongs to Nidoviridae, Coronaviridae, and is divided into three genera: α, β, and γ. The α and β genera are only pathogenic to mammals, and the γ genus mainly causes bird infection. CoV is mainly transmitted through direct contact with secretions or through aerosols and droplets, and there is also evidence that it can be transmitted through the fecal-oral route. [0003] So far, there are 7 human coronaviruses (HCoV) that cause respiratory diseases in humans: HCoV-229E, HCoV-OC43, SARS-CoV, HCoV-NL63, HCoV-HKU1, MERS-CoV and novel coronavirus (2019) , is an important pathogen of human respiratory tract infection. Among t...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/577
CPCG01N33/56983G01N33/577
Inventor 刘萍黄爱龙栾大伟张振斌李华侯玉文史光辉寇悦汪德强陈娟
Owner BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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