44 results about "Cepharanthine" patented technology

Cepharanthine is used as effective component in preparing medicine for resisting SARS virus. Within the safe dosage range, the present invention has high inhibition rate to SARS virus and powerful effect of preventing SARS virus infection. The medicine can alleviate the syndromes of SARS patient and has fast after cure, less toxicity, high stability and other advantages.

The invention relates to a method for separating and extracting cepharanthine, which comprises the following steps of: taking a dry stephania tuberous root, crushing, adding 2 to 3L of water and 0.5 to 5ml of mixed bio-enzyme liquid into each kilogram of crushed material, soaking for 1 hour, adding 9 to 11L of 1 to 2 mass percent aqueous solution of HCL, heating to 90DEG C, and soaking for 3 hours; and separating out a liquid medicine, adding 9 to 11L of 1 to 2 percent aqueous solution of HCL into dregs, decocting for 3 hours, filtering, mixing filtrate, adsorbing by adopting a cation exchange column or cation cellulose column, enriching the cepharanthine, eluting by using solution that sodium hydroxide is added into 60 to 90 percent aqueous solution of ethanol until the saturation state as eluent, collecting eluent of a cepharanthine section, regulating the pH value to be 7, concentrating for recovering ethanol, filtering, dissolving a precipitate by using absolute ethanol, eluting and purifying by using an anion column, collecting eluent of a cepharanthine section, concentrating, crystallizing, and drying to obtain the cepharanthine. The method has the advantages of simple operation, light pollution, low cost, high yield, high purity of cepharanthine, and suitability for industrial production.

The invention relates to application of stephanine and hydrochloride thereof in the field of medicine, namely the stephanine or cepharanthine hydrochlofide is taken as an effective ingredient to prepare an anti-hepatitis B virus drug. The research shows that: the median toxic concentration (TC50) of the CH to cells is 2.09mu g.ml; and the CH can inhibit the synthesis of HBV DNA in the cells (P is less than 0.01) and is dose-dependent, the inhibition ratio of the CH to the HBV DNA in supernatant fluids of the cells is more than 50 percent when the dose is more than 0.5mu g.mL, IC50 of the CH is 0.324mu g.mL, the therapeutic index TI is equal to 6.45 (the TI is more than 2.0 and less than 10.0), and the CH has stronger effect of inhibiting the synthesis of the HBV DNA and is an effective low-toxicity drug. The invention develops novel pharmaceutical application of the stephanine and the hydrochloride of the stephanine, has the advantages of rich source of preparation raw materials, low cost, small toxic side effect, and simple preparation technology, can be prepared into oral dosage forms, injection dosage forms, tablets and the like, and has convenient use.

The invention relates to a cepharanthine nanosuspension and a preparation method thereof. The cepharanthine nanosuspension is obtained through nano-treatment of cepharanthine and a stabilizer aqueoussolution, wherein the stabilizer is one or any combinations of a space stereo stabilizer, a charge stabilizer and a super disintegrating agent. The prepared celastatin suspension has the advantages ofhigh dissolution and high bioavailability. The celastatin suspension can be used as a finished preparation or a preparation intermediate for preparing a solid / liquid cepharanthine preparation, and the stable absorption and effects of the drug can be both ensured. Moreover, the technology is feasible, stable and reliable, the production cost is low, and the scale industrial production is easy to achieve.

The invention provides a coronavirus separated from pangolin. The coronavirus is named as pangolin coronavirus xCoV, the homology of the coronavirus xCoV and S protein of SARS-COV-2 reaches 92.5%, receptors of xCoV infected cells and the SARS-COV-2 are consistent and are angiotensin converting enzyme 2 (ACE2). However, the xCoV does not infect people, is very safe to people, can be used for screening active drugs and vaccines for resisting the SARS-COV-2 virus, and can also be used for preparing attenuated vaccines or inactivated vaccines for resisting the SARS-COV-2 virus. Based on the pangolin coronavirus xCoV, a plurality of active compounds with anti-coronavirus activity are obtained by screening, EC50, CC50 and SI evaluation is performed on cepharanthine (stephanine), silamectin and mefloquine hydrochloride (mefloquine) in the active compounds, and the xCoV inhibition mechanism of cepharanthine is analyzed and researched through transcriptome sequencing.

The invention relates to a safe and high-efficiency pilatory and the preparation method thereof. The pilatory is made from 40 to 80 parts of cepharanthine, 5 to 15 parts of a red sage root extract, 10 to 30 parts of total saponin in notoginseng, 5 to 15 parts of a polygonum extract, 10 to 20 parts of a cochinchnese asparagus root extract, 5 to 15 parts of a prepared rehmannia root extract, 15 to 30 parts of a Chinese angelica root extract and 5 to 15 parts of ecdysone. Due to stronger permeability, the pilatory can be infiltrated to the root part of hair and stimulate the cell tissues of the root part of the hair to regenerate new hair by utilizing the specific medical speciality of the Chinese medicinal herbs. Meanwhile, the pilatory can improve the function of the scalp capillary, promote the blood circulation of the scalp, increase the nutrients needed by hair growth and quicken hair growth.

The present invention relates to a preparation method of cepharanthine hydrochloride, which discloses a purification and synthesis process of cepharanthine hydrochloride. The method is simple and controllable, and saves energy source. The prepared cepharanthine hydrochloride has reliable quality and high bioavailability, can remarkably relieve chemotherapy and radiotherapy-induced leukocytopenia of patients with malignant tumors, and has good application prospects.

The invention relates to cepharanthine hydrochloride injection, and a preparation method and a detection method for the cepharanthine hydrochloride, discloses injection which utilizes cepharanthine hydrochloride as raw materials and comprises auxiliary materials received by pharmaceutics, and further discloses the detection method for the injection and clinical application of the injection. The cepharanthine hydrochloride injection is simple and feasible in preparation process and high in biological availability, is safe and effective, can remarkably reduce leucopenia of patients with malignancy due to chemotherapy and radiotherapy, and has excellent application prospect.

The invention relates to the technical field of biologics, and discloses application of cepharanthine in preparing an in-vitro amplification culture medium of hemopoietic stem cells of placentae and / or in in-vitro amplification of the hemopoietic stem cells of the placentae, and a culture medium containing cepharanthine amplified hemopoietic stem cells of placentae, and a method. As multiple appropriate components are combined with the cepharanthine to prepare the in-vitro amplification culture medium, the amplification effect of the in-vitro amplification culture medium can be remarkably improved in in-vitro amplification of the hemopoietic stem cells of the placentae, and the in-vitro amplification culture medium has the characteristics of quick in dryness and high in activity.

The invention relates to (1R,1'R)-cepharanthine represented by a chemical structural formula 1 shown in the description, a preparation method therefor and use of the (1R,1'R)-cepharanthine. The (1R,1'R)-cepharanthine is extracted from Epigeal Srephaia tubers. The (1R,1'R)-cepharanthine has good inhibiting activity to human cervical carcinoma cells (Hela), human lung cancer cells (A549) and human mammary cancer cells (MCF-7) and can be used for preparing antitumor drugs. A third aspect of the technical scheme of the invention is to provide an application of the (1R,1'R)-cepharanthine described in a first aspect in the preparation of the antitumor drugs.

The invention relates to the technical field of biological medicine, in particular to a chemotherapy pharmaceutical composition and application thereof. The invention relates to a chemotherapy pharmaceutical composition, which comprises cepharanthine and epirubicin. Before epirubicin is administrated, cepharanthine is administrated firstly, and the cancer cell inhibiting effect of epirubicin can be synergistically enhanced. According to the scheme, the technical problems that the curative effect of epirubicin is limited, and adverse reactions and drug resistance are easily generated when a cancer patient uses epirubicin for a long time are solved. The composition can be applied to clinical practices of treatment of various cancers, reduces adverse reactions of epirubicin, and prevents drugresistance of epirubicin at the same time.

The invention relates to the technical field of biology, and discloses the application of cepharanthine in the preparation of in-vitro amplification culture medium of hematopoietic stem cells and / or in-vitro amplification of the hematopoietic stem cells, a cepharanthine-containing culture medium for amplifying umbilical cord blood stem cells and a method. According to the culture medium provided by the invention, the in-vitro amplification culture medium is composed of multiple appropriate components and the cepharanthine, and the amplification effect can be obviously improved during in-vitro amplification of the hematopoietic stem cells, so that the culture medium has the characteristics of high dryness and high activity.

A treatment method including: (a) identifying a subject in need of treatment for androgenic alopecia (AGA), and (b) orally administering a physiologically effective amount of cepharanthine (CEP) to the subject according to a dosage regimen.

The invention relates to the technical field of medicines and provides application of cepharanthine and salts thereof as a ferroptosis inducer in preparation of an anti-tumor drug. As found, natural products, namely cepharanthine and salts thereof, which are already applied clinically for many years can induce ferroptosis of prostate cancer cells in expressive ways of active oxygen increase, accumulation of lipid peroxides, decrease in the expression level of GPX4, structural changes in mitochondria, etc. Therefore, cepharanthine and salts thereof have good anti-cancer effects and provide a new effective therapeutic drug for tumor disease patients.

The invention belongs to the technical field of medicines, and particularly relates to application of combined stephanine and an autophagy inhibitor for preparing drugs for treating a liver cancer. Itis found that drug combination of the cepharanthine hydrochloride and the autophagy inhibitor has a good cancer inhibition effect in vitro and vivo, more treatment solid tumor schemes are provided, and two combined drugs are wide in source and are economic and effective. According to the application of the combined stephanine and the autophagy inhibitor for preparing the drugs for treating the liver cancer, it is firstly found that by using the autophagy inhibitor to reinforce the antitumor effect of the cepharanthine hydrochloride in vitro and vivo, the antitumor effect of the cepharanthinehydrochloride in vitro and vivo is verified on a vivo mouse model, and a novel scheme for effectively and feasibly treating a solid tumor is provided.

The invention discloses a plant enzyme mask as well as a preparation method and application thereof. The plant enzyme mask is prepared from the following components in parts by weight: 12 to 18 partsof fruit and vegetable enzyme powder, 7 to 11 parts of dihydromyricetin, 6 to 12 parts of cepharanthine, 10 to 20 parts of hypericin, 5 to 17 parts of hydrolyzed yolk powder, and 2 to 7 parts of fucoidin. The plant enzyme mask has the advantages that the plant enzyme mask is prepared from the main raw materials of fruit and vegetable enzyme powder, dihydromyricetin, cardamine, hypericin, hydrolyzed yolk powder and fucoidin, the easiness in acne forming is avoided; the better acne removal effect is realized; the plant enzyme mask is suitable for acne skin to use; the easiness in allergy in theuse process is avoided, the irritation property is small, the use feel is good, and the plant enzyme mask is suitable for being popularized.

The invention relates to the technical field of biology, and particularly relates to a hemopoietic stem cell medium. The medium includes a base medium and an additive added into the base medium. Based on final concentrations, the additive includes 100-200 mg / mL of FBS, 0.02-0.03 [mu]mol / mL of sodium selenite, 5-10 mg / mL of bacillus calmette-guerin polysaccharides and nucleic acids, 50-100 [mu]mol / mL of cepharanthine, 50-100 ng / mL of trehalose, 40-80 ng / mL of vitamin C, 1.5-2.5 mg / mL of a first cytokine slow-release microcapsule, and 25-50 mg / mL of a second cytokine slow-release microcapsule. The hemopoietic stem cell medium can significantly increase the propagation rate and cell activity of hemopoietic stem cells, allows the hemopoietic stem cells to be in a propagation undifferentiated state for long time, and maintains stem cell characteristics of the hemopoietic stem cells.

The invention relates to a preparation method of an L-cepharanthine sustained-release microsphere. The preparation method comprises the following steps: dissolving L-cepharanthine and a degradable polymer containing a hydrophobic chain segment in an organic solvent to obtain a drug-containing polymer solution; under a stirring condition, adding the drug-containing polymer solution into a water phase to form an O / W type emulsion and removing the organic solvent in the emulsion; wherein the water phase contains a stabilizer and a ratio of the stabilizer to the water phase is 0.1%-5% (w / v); and under the stirring condition, adding the obtained dispersion system into water to be cured to obtain a L-cepharanthine sustained-release microsphere. The L-cepharanthine sustained-release microsphere has a proper particle size, is round and free of a burst release phenomenon, and has a long-acting anti-depression effect.

The present invention relates to five kinds of improved cepharanthine medicine preparation, including soft cepharanthine capsule, hard cepharanthine capsule, dispersed cepharanthine tablet, cepharanthine for injection and cepharanthine liquid for injection. These cepharanthine medicine preparations have the features of extracorporeal disintegration, complete absorption, high bioavailability and high clinical treating effect.

The cepharanthine dripping pill containing cepharanthine 1-10 mg and proper amount of dripping pill medium in each pill. The dripping pill medium is one or two of polyglycol-6000, polyglycol-4000, polyoxyethylene monostearate, sodium stearate, glycerin, gelatin, poloxamer, stearic acid, glycerin monostearate and insect wax. The preparation process of the cepharanthine dripping pill includes melting and mixing cepharanthine and dripping pill medium, dropping at 60-100 deg.c into condensating agent, and eliminating condensating agent. The condensating agent is one or two of dimethyl silicon oil, liquid paraffin, tea seed oil and rape seed oil. The cepharanthine dripping pill is white or slight yellow and bitter. The cepharanthine dripping pill is used in treating chemotherapy caused leucopenia, and has the features of less side effect, low dosage, high medicinal effect, etc.

The invention belongs to the field of detection, and particularly relates to a serum-free medium capable of amplifying mesenchymal stem cells and a preparation method thereof. The serum-free medium mainly contains curcumin, cepharanthine, berberine, resveratrol, anthocyanidin, bone morphological protein 4, beta-glycerophosphate, cholesterol, isobutyl methyl xanthine, EGF, biotin, aFGF, heparin sodium, reduced glutathione, transferrin, insulin, ascorbic acid, dexamethasone, interleukin 2, vitamin B12, interleukin 6, stem cell factors, nicotinamide, TGF-beta 3, thrombopoietin, taurine, rosiglitazone, FMS-like tyrosine kinase 3 ligand, IGF2, sodium pyruvate, GM-CSF, TPO and the like. The serum-free culture medium not only has the capacity of amplifying mesenchymal stem cells derived from fat, placenta, bone marrow and umbilical cord in vitro, but also can maintain self-renewal of the stem cells, prevent differentiation of the stem cells and inhibit canceration of the stem cells.

The invention provides a tissue culture breeding method of stephania epigaea high in cycleanine and cepharanthine content. The method includes the following steps that after being selected and sterilized, stephania epigaea explants are inoculated to an axillary bud induction culture medium, and obtained axillary buds are cut off and transferred to a culture medium for bud callus induction; then, bud calluses are transferred to an adventitious bud culture medium to induce differentiation of adventitious buds, and the number of the adventitious buds reaches 10-20 within 40 days; ancymidol is added to inhibit elongation of the buds and induce rooting, and after seedling hardening, transplanting is carried out, wherein the survival rate reaches 90% or above. The method is suitable for industrial production and has the advantages of being high in breeding speed and large in amplification factor, maintaining excellent characters of original varieties and the like.

The invention belongs to the field of medicines and in particular relates to a cepharanthine-containing anti-inflammation medicine composition and a preparation method thereof. The anti-inflammation medicine composition provided by the invention comprises the following components in percentage by weight: 1-2% of a herba rabdosiae extract, 1-2% of a common andrographis herb extract, 2-3% of cepharanthine, 0.2-0.4% of essence oil, 0.1-0.3% of an emulsifier and 92.3-95.7% of purified water. Since an isoquinoline alkaloid compound, namely the cepharanthine extracted from delavay stephania roots, is added into the anti-inflammation medicine composition provided by the invention, the anti-inflammation performance of a medicine can be improved, no stimulation reaction can be caused for human bodies, and probability is provided for developing a type of novel anti-inflammation medicines.

A dripping pill of stephanine for treating the leukocytopenia caused by radiotherapy and chemicotherapy is prepared from stephanine and medical carrier.

The invention discloses a novel application of Cephaaranthine in treatment of rheumatoid arthritis, and belongs to the field of traditional Chinese medicines. The cepharanthine disclosed by the invention is a bisbenzylisoquinoline compound extracted from a traditional Chinese medicinal material stephania cepharantha, and shows a good treatment effect on a mouse model of inflammatory arthritis induced by collagen; in addition, the cepharanthine has a certain inhibition effect on the cell viability, migration ability and signal transduction pathway of fibroblast-like synovial cells of rheumatoid arthritis patients, which indicates that the cepharanthine has an exact effect of resisting osteoarthritis inflammatory diseases such as rheumatoid arthritis and the like; the compound can be combined with auxiliary materials to prepare anti-rheumatoid arthritis and other inflammatory diseases or be combined with other drugs for treating rheumatoid arthritis.

The present invention relates to a kind of orally disintegrating cepharanthine tablet, which has the functions of promoting reticuloendothelial system, resisting allergic reaction, etc. and is used in preventing and treating leucopenia caused by radiotherapy and chemotherapy, and its preparation process. The orally disintegrating cepharanthine tablet is prepared with cepharanthine as main material and stuffing, disintegrating agent, corrective, flow assistant, lubricant, adhesive or coating material, effervescent agent and other supplementary material, and through tablet pressing in a tablet press. The orally disintegrating cepharanthine tablet has high friability, fast disintegrating, good taste, low production cost and other advantages.

The invention provides a seed breeding method of stephania epigaea high in cycleanine and cepharanthine content. The method includes the steps of collection and screening of high-content female and male stephania epigaea plants, hybridization, seed collection, seeding matrix preparation, seed treatment, sowing, strong seedling formation, disease and pest prevention, transplanting and the like. Bymeans of seed breeding, industrial production of the stephania epigaea high in medicinal component content can be effectively achieved, and the conflict between increasing market demands and gradual exhaustion of wild resources is relieved. The method has the advantages of being simple, easy to implement, low in cost, high in breeding speed and the like.