Serum-free culture medium capable of amplifying mesenchymal stem cells and preparation method thereof
A technology of serum-free medium and mesenchymal stem cells, which is applied in the field of serum-free medium capable of expanding mesenchymal stem cells and its preparation, can solve the problems of interfering with vitamin absorption, affecting the effect of stem cell transplantation, and uneven nutrition, so as to maintain self- Renewal, increase cell adhesion performance, and inhibit canceration
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment
[0043] Embodiment, preparation of a serum-free medium capable of expanding mesenchymal stem cells
[0044] The serum-free medium for expanding mesenchymal stem cells uses DMEM / F12 medium as a solvent, and consists of the following components and their concentrations, see Table 1:
[0045] The composition and the concentration composition of the serum-free medium of table 1 embodiment 1~7
[0046]
[0047]
[0048] The preparation method is:
[0049] Take 100 grams of DMEM / F12 medium powder, add 900 mL of deionized water, and then add 3 g of NaHCO 3 , add 3g 4-hydroxyethylpiperazineethanesulfonic acid (Hepes), stir for 1h, adjust the pH value to 7.2, add the following ingredients: curcumin, stepherine, berberine, resveratrol, anthocyanins, Bone morphoprotein 4, β-glycerophosphate, cholesterol, isobutylmethylxanthine, EGF, biotin, aFGF, heparin sodium, reduced glutathione, transferrin, insulin, ascorbic acid, dexamethasone, interleukin 2, vitamins B12, interleukin 6, st...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com