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Seed breeding method of stephania epigaea high in cycleanine and cepharanthine content

A technology of fenugreek and fenugreek, applied in the field of plants, can solve problems such as inability to meet the medicinal market, intensified human activities, threats to species survival, etc., and achieve the effects of reducing breeding time, reducing experimental costs, and convenient transportation.

Inactive Publication Date: 2019-07-05
YUNNAN BIOVALLEY PHARMA CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Ground tolerance and other plants of the subgenus of the subgenus of the mountain tortoise have garden ornamental and important medicinal values. In recent years, due to over-exploitation, wild species resources have decreased sharply. Moreover, the family Fangjiceae belongs to dioecious plants, and the reduction of species has caused female and male plants to grow. Difficulty in pollination, coupled with the low germination rate of seeds due to seed coat barriers under natural conditions, intensified human activities, environmental changes and other factors, are already facing endangerment, not only cannot meet the growing medicinal market, but also threaten the survival of the species

Method used

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  • Seed breeding method of stephania epigaea high in cycleanine and cepharanthine content

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Materials: ground seeds harvested from July to August 2017

[0037] 2. Experimental equipment: nutrition bag 10×10cm (diameter×height), beaker (250ml), glass rod, timer, tweezers.

[0038] 3. Method: use the corrosiveness of concentrated sulfuric acid to remove the seed coat, rinse the seeds with sterile water for many times until there is no concentrated sulfuric acid residue, and then sow them in the soil matrix. The experiment is 30 seeds each time, repeated 3 times.

[0039] 4. Operation steps:

[0040] Step 1: Harvesting of female and male plants in the wild with high content, based on the screening of the content of biologically active components of scutellaria and stephenin, collecting the tubers of the female and male plants and measuring the contents of scutellaria and stepherin , the content of transplanted stephatrin and sarcocarpus were greater than 3.5mg·g at the same time -1 , 2.4mg·g -1Female and male individual tubers are planted in a 4:1 ratio, wi...

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Abstract

The invention provides a seed breeding method of stephania epigaea high in cycleanine and cepharanthine content. The method includes the steps of collection and screening of high-content female and male stephania epigaea plants, hybridization, seed collection, seeding matrix preparation, seed treatment, sowing, strong seedling formation, disease and pest prevention, transplanting and the like. Bymeans of seed breeding, industrial production of the stephania epigaea high in medicinal component content can be effectively achieved, and the conflict between increasing market demands and gradual exhaustion of wild resources is relieved. The method has the advantages of being simple, easy to implement, low in cost, high in breeding speed and the like.

Description

technical field [0001] The invention relates to a method for breeding seeds with incompatible contents of takaninin and stephenin, belonging to the field of plants. [0002] technical background [0003] Stephania epigaea is a subgenus of stephania (Stephania) in the family Menispermaceae. This genus has huge roots and is exposed on the ground and looks like a turtle. Therefore, it is also called "mountain turtle". It is a traditional Chinese herbal medicine in my country. , its root tubers are used as medicine, and are widely used in various places south of the Yangtze River Basin, especially among ethnic minorities in South China and Southwest China. [0004] Ground tolerance and other plants of the subgenus of the subgenus of the mountain tortoise have garden ornamental and important medicinal values. In recent years, due to over-exploitation, wild species resources have decreased sharply. Moreover, the family Fangjiceae belongs to dioecious plants, and the reduction of spe...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01H1/02
CPCA01C1/00A01H1/02
Inventor 林艳和杜江李村富林春刘正杰毛自朝
Owner YUNNAN BIOVALLEY PHARMA CO LTD
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