Sample pad processing liquid, H7 subtype avian influenza virus colloidal gold test strip and preparation method thereof

Abstract

The invention discloses a sample pad processing liquid, a H7 subtype avian influenza virus colloidal gold test strip and a preparation method thereof, the processing liquid is prepared as follows: taking 8-20g of PEG(polyethylene glycol)-200, 11-25g of PVP (polyvinyl pyrrolidone), 10-45g of BSA (bovine serum albumin), 0.5-30ml of Triton X - 100, and 0.8-15ml of Twain-20, then using 5mM-10mM phosphate buffer solution to fix the volume to 1L, and adjusting the pH value to 9.5; and the colloidal gold test strip is formed by overlapping and pasting in turn a sample pad, a glass fiber membrane, a cellulose nitrate membrane and absorbent paper on a bottom plate, and the sample pad is processed by the processing liquid. The processing liquid can increase the sample pad hydrophilicity, contributes to the sample pad fast wetting, and promotes the chromatographic effect, aqueous solution formed by dissolving large molecules in water has suspension and dispersion effect, and can protect virus antigen in a sample. The test strip has the advantages of safe and simple operation, strong specificity, high sensitivity, and low rate of cross infection.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a sample pad treatment liquid, H7 subtype avian influenza colloidal gold test strips and a preparation method thereof. Background technique [0002] Currently existing test strips, the common components of the sample pad treatment solution are mainly blocking substances (such as: bovine serum albumin, casein) and surfactants (Tween-20, S-17, S-9, Triton X-10 ), among them, commonly used proteins (such as: bovine serum albumin), which have the effect of flow blocking and protecting gold-labeled antibodies; Triton X-10, Tween-20 surfactants can improve hydrophilicity and wettability , to reduce the surface tension of the liquid, which is conducive to the rapid and sufficient reaction of the antigen in the detection sample and the gold-labeled antibody in the test strip, and the wettability effect depends on the compatibility of the surfactant with other polymer matrices; however, dif...

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Problems solved by technology

[0004] However, the current detection of avian influenza virus requires expensive instruments and equipment, and the detection cost is high. It must have a special laboratory and professional operators; therefore, it is not suitable for the detection site, detection equipment and professional operating conditions. In airports, ports, railway stations, hospitals and other places with high population density and high flow of people; it is also not suitable for on-site instant detection in grassroots communities, blood stations, border inspection and quarantine or households with simple equipment; and the operation is cumbersome and the detection cycle It takes 120 minutes for the detection reaction alone, which cannot meet the needs of large sample volume and real-time detection, and directly affects the speed of epidemic prevention and control; and there is no test strip for detecting H7 subtype avian influenza virus, so it is simple and fast to establish Methods to detect H7 subtype avian influenza viruses are critical to help monitor and prevent their spread

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