Kit for detecting creatine kinase isoenzyme and preparation and use methods thereof

Abstract

The invention relates to a kit for detecting creatine kinase isoenzymes and preparation and use methods thereof. The kit for detecting creatine kinase isoenzymes comprises a substrate, as well as a sample pad, a conjugate pad, a coupling pad, a nitrocellulose membrane and water absorbent paper, which are arranged from one end to the other end on the substrate, wherein the conjugate pad is coated with a conjugate of fluorescence silicon dioxide nanoparticle-anti-creatine kinase isoenzyme MB monoclonal antibody, the nitrocellulose membrane is provided with a test line and a quality control line, the test line is arranged on the side close to the coupling pad and contains an anti-creatine kinase isoenzyme MB monoclonal antibody, and the quality control line is arranged on the side away from the coupling pad and contains goat anti-mouse IgG (Immunoglobulin G). The kit is low in price and has simple preparation process, and works with a high detection speed to provide the detection result within 15 minutes. The portable fluorescence detection device is adopted to achieve the purpose of accurate quantification of a target analyte. The kit is convenient to operate, can be applied to on-site detection, and is suitable for operators who do not need training. The kit has the advantages of high sensitivity, good specificity and accurate result, and is convenient for generalization and application.

Description

technical field [0001] The invention relates to a protein detection method and a kit, in particular to a kit for detecting creatine kinase isozyme and a preparation method thereof, and also to a method for using the kit to detect creatine kinase isozyme. Background technique [0002] Myocardial injury markers refer to biochemical substances that can be measured in circulating blood and can sensitively and specifically reflect myocardial injury (abnormality) and its severity, so they can be used for screening of myocardial injury (abnormality) , diagnosis, assessment of prognosis and follow-up treatment. Under normal conditions, myocardial injury markers are mainly or exclusively present in the heart (myocardium), and are released in large quantities by the heart under cardiac or cardiovascular abnormalities. Cardiac markers mainly include myoglobin, troponin (cTn I and cTn T), creatine kinase isoenzyme (CK-MB), etc. Among them, CK-MB has high diagnostic sensitivity for myo...

AI Technical Summary

Problems solved by technology

The detection sensitivity of enzyme-linked immunosorbent assay is high, but the operation process is relatively complicated, and the detection takes a long time; it needs special equipment such as microplate reader to cooperate with it, and the operator needs professional training; the cost of detection is relatively high. High, unable to achieve single-person detection

The chemiluminescence method has good precision and sensitivity, but requires special equipment, and the instruments and reagents are relatively expensive, so it is not suitable for small hospitals and medical centers

The operation of colloidal gold immunochromatography is simple, the time required for detection is short, no professional technicians are required to operate, and the detection cost is low. However, this method can only achieve qualitative or semi-quantitative detection, and the detection sensitivity is low, and it cannot detect creatine kinase isoenzyme

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