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Semi-quantitative rapid detection test paper for immunoglobulin G in bovine colostrum and products thereof and preparation method thereof

An immunoglobulin and detection test paper technology, applied in the field of immunoglobulin G semi-quantitative rapid detection test strips and their preparation, can solve the problems of cumbersome detection operation procedures, unsuitable for on-site operation, and long detection time, etc. High sensitivity, simple, fast, semi-quantitative and rapid detection, easy to operate

Inactive Publication Date: 2010-12-15
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the detection procedure of immunoglobulin G in the existing bovine colostrum and its products is cumbersome, the time required for detection is long, and it is not suitable for on-site operation. The present invention provides a kind of bovine colostrum and immunoglobulin G. Immunoglobulin G semi-quantitative rapid detection test strip in its product and preparation method thereof

Method used

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  • Semi-quantitative rapid detection test paper for immunoglobulin G in bovine colostrum and products thereof and preparation method thereof
  • Semi-quantitative rapid detection test paper for immunoglobulin G in bovine colostrum and products thereof and preparation method thereof
  • Semi-quantitative rapid detection test paper for immunoglobulin G in bovine colostrum and products thereof and preparation method thereof

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specific Embodiment approach 1

[0016] Specific implementation mode one: combine figure 1 Illustrate this embodiment, the test strip for semi-quantitative rapid detection of immunoglobulin G in bovine colostrum and its products in this embodiment is made of polyvinyl chloride (PVC) backing 1, sample absorbent pad 2, glass fiber 3, and nitrocellulose The plain film 4 and the absorbent pad 5 are composed of a sample absorbent pad 2, a glass fiber 3, a nitrocellulose membrane 4 and a absorbent pad 5 which are successively adhered to a vinyl chloride (PVC) backing 1, wherein the sample absorbent pad 2 and the absorbent pad are The glass fiber 3 is adjacent, the glass fiber 3 is overlapped with the nitrocellulose membrane 4, and the nitrocellulose membrane 4 is overlapped with the absorbent pad 5; the glass fiber 3 is coated with a rabbit anti-bovine IgG-colloidal gold marker; The nitrocellulose membrane 4 is coated with 1 to 4 detection lines 6 composed of rabbit anti-bovine IgG and 1 quality control line 7 comp...

specific Embodiment approach 2

[0021] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the nitrocellulose membrane is coated with 2 to 4 detection lines 6 composed of rabbit anti-bovine IgG, and the distance between two adjacent detection lines 6 is It is 2-4mm. Other parameters are the same as in the first embodiment.

[0022] In this embodiment, the amount of rabbit anti-bovine IgG coating on multiple detection lines has an increasing gradient, which can expand the detection range of immunoglobulin G in bovine colostrum or its products, while controlling the amount of rabbit anti-bovine IgG on the detection line. The increasing gradient of IgG coating amount can realize the detection range of immunoglobulin G in bovine colostrum or its products with different precision, and realize the semi-quantitative detection of immunoglobulin G in bovine colostrum or its products through simple and rapid on-site operation Quick check.

specific Embodiment approach 3

[0023] Specific embodiment three: the difference between this embodiment and specific embodiment one is that the nitrocellulose membrane 4 is coated with 1 to 4 detection lines 6 composed of rabbit anti-bovine IgG, and the coating amount of the rabbit anti-bovine IgG is 0.075 μg ~1 μg. Other parameters are the same as in the first embodiment.

[0024] In this embodiment, the coating amount of rabbit anti-bovine IgG on detection line 6 is designed according to the sensitivity and accuracy required for detection. When the number of detection lines 6 is greater than 1, the coating amount of rabbit anti-bovine IgG along the detection lines in the same direction changes gradiently. The width of each detection line 6 is 1 mm.

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Abstract

The invention discloses semi-quantitative rapid detection test paper for immunoglobulin G (IgG) in bovine colostrum and products thereof and a preparation method thereof, which relate to the semi-quantitative detection test paper for the IgG and the preparation method thereof and solve the problems of the complex detection operating procedure, long detection time and unsuitability for field operation of IgG in the conventional bovine colostrum and the products thereof. The test paper consists of a back lining, a sample absorbing pad, glass fiber, a cellulose nitrate membrane and a water absorbing pad, wherein the cellulose nitrate membrane is provided with 1 to 4 detection lines and 1 quality control line. The method comprises the following steps of: 1, preparing a rabbit anti-bovine IgG-colloidal gold marker and enveloping the marker on the glass fiber; 2, enveloping the detection lines and the quality control line on the cellulose nitrate membrane; and 3, assembling the test paper. The test paper has the advantages of high detection flexibility, minimum detectable amount of 10mu g / mL, high specificity, no cross reaction with IgG in ovine colostrum and products thereof, simple procedure, high detection speed, detection time of generally 15 to 20 minutes for one sample and suitability for field detection.

Description

technical field [0001] The invention relates to a semi-quantitative rapid detection test strip of immunoglobulin G and a preparation method thereof. Background technique [0002] Scientific research has found that the colostrum of dairy cows is most in line with the needs of the human body. The various growth hormones contained in it can regulate the immune function of the human body and help the growth and development of bones, muscles, nerves, and cartilage joints. With the rapid development of my country's dairy industry, the number of dairy cows on hand continues to increase, and the output of bovine colostrum continues to increase. According to statistics, at the end of 2005, there were 11 million dairy cows in my country. If the cow consumes 11kg, there will be 32.5kg of colostrum remaining per cow. If 60% of the cows give birth and calve each year, there will be 21,500 tons of colostrum to be processed and utilized every year. It can be seen that the output of colostru...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/544G01N33/532
Inventor 刘春龙李忠秋冯兴军任延铭
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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