Semi-quantitative rapid detection test paper for immunoglobulin G in bovine colostrum and products thereof and preparation method thereof
An immunoglobulin and detection test paper technology, applied in the field of immunoglobulin G semi-quantitative rapid detection test strips and their preparation, can solve the problems of cumbersome detection operation procedures, unsuitable for on-site operation, and long detection time, etc. High sensitivity, simple, fast, semi-quantitative and rapid detection, easy to operate
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specific Embodiment approach 1
[0016] Specific implementation mode one: combine figure 1 Illustrate this embodiment, the test strip for semi-quantitative rapid detection of immunoglobulin G in bovine colostrum and its products in this embodiment is made of polyvinyl chloride (PVC) backing 1, sample absorbent pad 2, glass fiber 3, and nitrocellulose The plain film 4 and the absorbent pad 5 are composed of a sample absorbent pad 2, a glass fiber 3, a nitrocellulose membrane 4 and a absorbent pad 5 which are successively adhered to a vinyl chloride (PVC) backing 1, wherein the sample absorbent pad 2 and the absorbent pad are The glass fiber 3 is adjacent, the glass fiber 3 is overlapped with the nitrocellulose membrane 4, and the nitrocellulose membrane 4 is overlapped with the absorbent pad 5; the glass fiber 3 is coated with a rabbit anti-bovine IgG-colloidal gold marker; The nitrocellulose membrane 4 is coated with 1 to 4 detection lines 6 composed of rabbit anti-bovine IgG and 1 quality control line 7 comp...
specific Embodiment approach 2
[0021] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the nitrocellulose membrane is coated with 2 to 4 detection lines 6 composed of rabbit anti-bovine IgG, and the distance between two adjacent detection lines 6 is It is 2-4mm. Other parameters are the same as in the first embodiment.
[0022] In this embodiment, the amount of rabbit anti-bovine IgG coating on multiple detection lines has an increasing gradient, which can expand the detection range of immunoglobulin G in bovine colostrum or its products, while controlling the amount of rabbit anti-bovine IgG on the detection line. The increasing gradient of IgG coating amount can realize the detection range of immunoglobulin G in bovine colostrum or its products with different precision, and realize the semi-quantitative detection of immunoglobulin G in bovine colostrum or its products through simple and rapid on-site operation Quick check.
specific Embodiment approach 3
[0023] Specific embodiment three: the difference between this embodiment and specific embodiment one is that the nitrocellulose membrane 4 is coated with 1 to 4 detection lines 6 composed of rabbit anti-bovine IgG, and the coating amount of the rabbit anti-bovine IgG is 0.075 μg ~1 μg. Other parameters are the same as in the first embodiment.
[0024] In this embodiment, the coating amount of rabbit anti-bovine IgG on detection line 6 is designed according to the sensitivity and accuracy required for detection. When the number of detection lines 6 is greater than 1, the coating amount of rabbit anti-bovine IgG along the detection lines in the same direction changes gradiently. The width of each detection line 6 is 1 mm.
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