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Method for extracting triple helix Dictyophora phalloidea polysaccharide

A technology of bamboo fungus polysaccharide and an extraction method, which is applied in the fields of biopolymers and medicinal chemistry, can solve problems such as fuzzy understanding of molecular internal structure, and achieve the effects of easy implementation, wide application prospect and clear structure.

Inactive Publication Date: 2009-08-12
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most scholars who study polysaccharides only stop at the extraction and purification of polysaccharides to obtain relatively high-purity sugars, and to determine the composition of monosaccharides, glycosidic linkages, etc., for the molecular internal structure. The understanding is vague

Method used

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  • Method for extracting triple helix Dictyophora phalloidea polysaccharide
  • Method for extracting triple helix Dictyophora phalloidea polysaccharide
  • Method for extracting triple helix Dictyophora phalloidea polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Pulverize the Dictyophora in a tissue grinder, degrease in a Soxhlet extractor with ethanol, ethyl acetate and acetone in sequence, and then extract with water at 20°C, 40°C, 60°C, and 80°C for three hours to obtain four kinds of The polysaccharide solution was processed as follows, and centrifuged to obtain the extract. After deproteinization by Sevage method, H 2 o 2 Oxidation decolorization, dialyzed with water and twice distilled water respectively, concentrated, graded precipitation with ethanol, freeze-dried to obtain triple helix Dictyophora polysaccharides ZS-1, ZS-2, ZS-3 and ZS-4. Freeze-dried to obtain pure polysaccharide.

Embodiment 2

[0023] Pulverize the Dictyophora in a tissue grinder, degrease in a Soxhlet extractor with ethanol, ethyl acetate and acetone in sequence, and then extract with water at 20°C, 40°C, 60°C, and 80°C for three hours to obtain four kinds of The polysaccharide solution was processed as follows, and centrifuged to obtain the extract. After deproteinization by Sevage method, H 2 o 2 Oxidative decolorization, dialyzed with clean water and twice distilled water respectively, concentrated, ultrafiltered, and freeze-dried to obtain triple helix Dictyophora polysaccharides ZS-1, ZS-2, ZS-3 and ZS-4. Freeze-dried to obtain pure polysaccharide.

[0024] The yields of the four polysaccharides ZS-1, ZS-2, ZS-3 and ZS-4 obtained in the above two examples are 0.444%, 0.364%, 0.243% and 0.388%, respectively.

[0025] The present invention uses GC to measure the monosaccharide composition of the four obtained products respectively, and finds that ZS-1 and ZS-2 are heteropolysaccharides, while ...

Embodiment 3

[0027] Embodiment 3: Measure the monosaccharide composition of each sugar

[0028] Use 2M sulfuric acid to hydrolyze and seal the polysaccharide, seal the tube and react at 100°C for six hours, use barium carbonate to neutralize the excess acid in the hydrolyzate, centrifuge to obtain the supernatant, freeze-dry, and use the method of sugar nitrile acetate derivatives to process the sample After the treatment, the monosaccharide composition was determined by gas phase.

[0029] Derivatization method: take 10 mg of solid sugar sample, 10 mg of hydroxylamine hydrochloride and 7 mg of internal standard, add 0.5 ml of pyridine, put it in a water bath at 90 ° C, heat and react for 30 minutes, take it out and cool it to room temperature, add 0.5 ml of acetic anhydride, and continue at 90 ° C The reaction was carried out for acetylation for 30 minutes, and the reaction product was directly analyzed by gas chromatography. The model of the gas chromatograph is Agilent GC6890N (Agilent...

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Abstract

The invention relates to an extraction method for triple helical edible fungus amylase, the specific steps of which are that firstly, crashed natural bamboo fungus is degreased with an organic solvent in a soxhlet extractor, secondarily, the residue is sequentially extracted with water in the gradient temperature of 20-80 DEG C, filtered to obtain filtrate, and repeated for three times; the supernatant fluid is taken by decentralization to obtain an extraction solution; the proteic extraction is carried out on the extraction solution by a Sevage method, and then the extraction solution is respectively dialyzed with clear water and secondary distilled water after being decolored by H2O2, and after the DEAE pillar removes the acidic polysaccharide, the solution is frozen and dried to obtain the white cotton-shaped triple helical edible fungus amylase. The extraction method is easy to be implemented; and the product has high purity, is discovered for the first time and verifies the triple helical structure of the edible fungus amylase, and has wide application prospect in the aspects of medicine and material.

Description

technical field [0001] The present invention relates to the fields of biopolymers and medicinal chemistry, in particular to a preparation method of a polysaccharide derivative of Dictyophora, identification of its structure and pharmacological action in biological activity. Background technique [0002] Studies in recent years have found that bamboo fungus contains a variety of amino acids, vitamins, polysaccharides and various inorganic salts. In addition to its nutritional value, it also has a certain role in disease prevention and health care. Polysaccharide is one of the important components in the fruiting body of Dictyophora. There have been many reports on the physiological activities and functions of polysaccharides, but whether the various biological activities of Dictyophora are related to the polysaccharides contained in it has not been reported yet. It has been reported that Dictyophora polysaccharide was isolated from Dictyophora fruiting bodies by hot water ex...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 陈敬华王家堂郑化
Owner WUHAN UNIV OF TECH
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