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Fish collagen repair sponge and preparation method thereof

A technology of fish collagen and fish skin collagen, which is applied in the field of collagen sponge and its preparation, can solve the problems of uncontrollable cytotoxicity, poor biocompatibility, and low bioactivity, and achieve biocompatibility and high porosity , Uniform and moderate pore size

Pending Publication Date: 2017-05-31
QINGDAO CHENLAND MARINE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chinese patent CN103772734A discloses a preparation method of mammalian-derived high-purity collagen sponge, which covers the entire process of collagen extraction, purification, cross-linking and freeze-drying, and its main purpose is first to obtain high-purity collagen, and then use it to prepare collagen sponge, but because it is derived from mammals, its cost is too high, which limits its industrial production to a certain extent; invention patent CN103007336A discloses a fish skin collagen-based composite sponge and its The preparation method, which uses fish skin collagen and chitosan as raw materials, freeze-dried and then cross-linked and then freeze-dried for the second time. This patent uses chitosan and collagen to prepare sponges, and chitosan is biocompatible Compared with collagen, it can only be used as a body surface material, and cannot be used as an internal repair material; the invention patent CN103525097A discloses an aquatic fish skin gelatin sponge and its preparation method
However, its gelatin has lost the unique triple helical structure of collagen, so its biological activity is far lower than that of collagen, which limits its application in biomedicine. uncontrollable cytotoxicity

Method used

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  • Fish collagen repair sponge and preparation method thereof
  • Fish collagen repair sponge and preparation method thereof
  • Fish collagen repair sponge and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of collagen raw material:

[0031] Take fish skin, clean and dehydrate, add 400ml 10% n-butanol to degrease, stir at 0-4°C for 8 hours, add 0.05M NaOH solution and stir for 10 hours, then fully wash with deionized water and treat with alkali solution fish skin until neutral.

[0032] Put the washed fish skin into a 0.5M acidic solution at a material-to-liquid ratio of 1:5 (w / w), add protease and stir for 24 hours, enzymatically treat it to obtain enzymatic collagen solution, and finally add 200ml of absolute ethanol Solution, finally the ethanol content in the collagen solution reaches 70%, stand still, filter to obtain collagen, then dehydrate with absolute ethanol, dry in low temperature vacuum to obtain collagen powder, and obtain medical grade biomaterials with molecular weight up to 300kD. Polymer fish skin collagen.

[0033] Preparation of collagen repair sponge:

[0034] Take the above-mentioned freeze-dried fish skin collagen raw material, dissolv...

Embodiment 2

[0039] Preparation of collagen raw material:

[0040] Take the fish skin, clean it and dehydrate it, add 600ml of 10% n-butanol to degrease, stir at 0-4°C for 12 hours, then add 0.2M NaOH solution and stir for 8 hours, then fully wash it with deionized water and treat it with alkali solution fish skin until neutral.

[0041] Put the washed fish skin into a 0.5M acidic solution at a material-to-liquid ratio of 1:10 (w / w), add protease and stir for 24 hours, enzymatically treat it to obtain an enzymatic collagen solution, and finally add 100ml of absolute ethanol Solution, finally the ethanol content in the collagen solution reaches 50%, stand still, filter to obtain collagen, then dehydrate with absolute ethanol, dry in low temperature and vacuum to obtain collagen powder, and obtain medical grade biomaterials with a molecular weight of up to 300kD. Polymer fish skin collagen.

[0042] Preparation of collagen repair sponge:

[0043] Take the above-mentioned freeze-dried fish...

Embodiment 3

[0047] The preparation method of the fish collagen raw material is the same as in Example 2.

[0048] Preparation of collagen sponge:

[0049] Take the above-mentioned freeze-dried fish skin collagen raw material, dissolve it completely with glacial acetic acid, the solution solubility is 15 mg / ml, measure its pH to be 5, let it stand at 25°C for 24 hours, and carry out self-assembly of micro-nano structure.

[0050] The above self-assembled collagen solution was poured into a mold, and vacuum freeze-dried at -80° C. for 36 hours to obtain a fish collagen sponge.

[0051] Immerse the freeze-dried fish collagen sponge in 1% genipin solution for cross-linking for 48 hours, then wash it with 1% glycine, finally wash it with purified water, freeze-dry it again, and then pack it and sterilize it by irradiation After processing, the fish skin collagen repair sponge is obtained.

[0052] Sterilize the above-mentioned collagen sponge for in vitro cell experiments, the operation meth...

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Abstract

The invention relates to the field of biomedical materials, and particularly provides a fish collagen repair sponge and preparation method thereof. The preparation method comprises the following steps: using fish skin as a raw material; degreasing, enzymatically hydrolyzing, purifying and filtering to obtain a fish collagen raw material; using the fish collagen raw material as the raw material for re-dissolution and self-assembly treatment; and then, freeze-drying and cross-linking to obtain the fish collagen repair sponge. The invention provides the fish collagen repair sponge which is simple in preparation process and low in price and overcomes some shortcomings of the existing collagen sponge. The fish collagen repair sponge utilizes a unique triple-helix structure of the fish collagen, has an excellent microstructure through self-assembly to a certain degree, has the advantages of high porosity, controllable mechanical properties and good biodegradability, and is an excellent biomedical material.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a collagen sponge and a preparation method thereof, in particular to a collagen sponge extracted from fish skin to prepare a biomedical sponge suitable for soft tissue repair with high porosity and adjustable mechanical properties. material, and the sponge is suitable for the repair treatment of burn defects, ulcers, trauma wounds and the like. Background technique [0002] Collagen is one of the basic components of human tissue, and about 1 / 3 of the total protein in the human body is collagen. Collagen itself is widely distributed in various organs of the body, such as skin, bones, cornea and so on. When collagen is damaged, the functions of many organs (such as skin, bones, etc.) are also affected. In the biomedical industry, collagen is one of the most critical and demanded biomedical materials. There are many types of collagen, and 26 types have been found so far, which c...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/56A61L27/58A61L27/36
Inventor 林莎莎杨毅李乘风
Owner QINGDAO CHENLAND MARINE BIOTECH CO LTD
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