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Method of extracting collagen and method of using collagen to prepare collagen protein

A technology of collagen and collagen, which is applied in the field of preparation of small molecular weight collagen, can solve the problems of collagen structure destruction, high extraction temperature, non-concentrated product molecular weight distribution, etc., and achieve the effect of maintaining stability and improving purity

Inactive Publication Date: 2007-01-31
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these methods only involve the extraction or preparation of a single collagen or collagen; they do not contain pre-extraction pretreatment procedures, and the obtained product contains more impurities, and the extraction temperature is higher, the extraction time is longer, and the specific structure The collagen structure is destroyed and denatured collagen is obtained; the molecular weight distribution of the product obtained during the enzymatic hydrolysis process is not concentrated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method of extracting collagen is as follows:

[0030] (1) Use tilapia skin as raw material, cut the fish skin into pieces, add n-hexane according to the mass volume ratio of fish skin to n-hexane of 1:10 g / ml, and degrease at a temperature of 25° C. for 72 hours;

[0031] (2) After degreasing, use 0.05mol / L NaOH solution to remove impurities at 25°C for 5 hours, and repeat twice;

[0032] (3) extract collagen with acetic acid solution, the concentration of acetic acid solution is 0.6mol / L, the mass volume ratio of the fish skin after impurity removal protein and acetic acid solution is 1: 10g / ml, extraction time is 48h, extraction temperature is 30 ℃, repeat the extraction twice;

[0033] (4) Add water according to the mass volume ratio of the extract to water 1:2.5g / ml, wash with water, purify, concentrate, freeze-dry to obtain collagen, and the yield of collagen is 44%.

[0034] The method for preparing low molecular weight collagen from extracted collagen is as...

Embodiment 2

[0039] The method for extracting collagen is the same as in Example 1.

[0040] The method for preparing low molecular weight collagen from extracted collagen is as follows:

[0041] (1) Add the extracted collagen to 6 times of water to dissolve, and then use trypsin for enzymolysis. The enzymolysis conditions are: E / [S] is 500u / g, enzymolysis time is 90min, and enzymolysis temperature is 40 °C, pH 8.0;

[0042] (2) After enzymatic hydrolysis, heat up to 95°C and keep warm for 8 minutes;

[0043] (3) Hydrolyzed collagen is obtained after concentration and spray drying, and the obtained collagen accounts for 91% of the total collagen mass with a molecular weight of 500-20000, and wherein the collagen protein with a molecular weight of 2000-7000 accounts for 43% of the total collagen mass .

Embodiment 3

[0045] The method for extracting collagen is the same as in Example 1.

[0046] The method for preparing low molecular weight collagen from extracted collagen is as follows:

[0047] (1) Add the extracted collagen to 6 times of water to dissolve, and then use trypsin for enzymolysis. The enzymolysis conditions are: E / [S] is 900u / g, enzymolysis time is 90min, and enzymolysis temperature is 45 °C, pH 7.5;

[0048] (2) After enzymatic hydrolysis, heat up to 100°C and keep warm for 5 minutes;

[0049] (3) Hydrolyzed collagen is obtained after concentration and spray drying. The obtained collagen accounts for 90% of the total collagen mass with a molecular weight of 500-20,000, and 44% of the total collagen mass with a molecular weight of 2,000-7,000. .

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Abstract

The present invention discloses a method for extracting collagen and method for preparing collagen protein by using said collagen. Said method for extracting collagen includes the following steps: using Java tilapia skin as raw material, cutting said Java tilapia skin, adding n-hexane, defatting, using NaOH solution to remove heteroprotein, using acetic acid solution to extract collagen protein, concentrating and freeze-drying so as to obtain collagen. The method for preparing collagen protein by using extracted collagen includes the following steps: adding water to dissolve the collagen, then utilizing proteinase to make zymolysis, concentrating and spray-drying so as to obtain hydrolytic collagen protein.

Description

technical field [0001] The invention belongs to the field of collagen and collagen protein preparation, and in particular relates to a method for extracting collagen by using leftover fish skin produced in the tilapia processing process as a raw material and a method for preparing low-molecular-weight collagen by using the extracted collagen as a raw material. Background technique [0002] Collagen and collagen protein are used in important fields of medicine and cosmetics. Collagen has strong biological activity, weak antigenicity and good biocompatibility, and has been widely used in medical and health fields such as burns, trauma, corneal diseases, cosmetology, orthopedics, hard tissue repair, and wound hemostasis. Collagen has the functions of moisturizing, repairing, anti-aging, nutrition and so on. Collagen can promote the synthesis of skin collagen, promote collagen metabolism, and can significantly improve the effect of low collagen synthesis related to aging. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H1/06C07K14/78
Inventor 曾庆孝宁初光朱志伟阮征张立彦李汴生
Owner SOUTH CHINA UNIV OF TECH
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