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Process for preparing antibiotic-peptide modified-fibroin film material

A silk protein and antimicrobial peptide technology, applied in medical science, absorbent pads, bandages, etc., can solve problems such as the emergence of immature technology, low antibiotics, and human side effects, and achieve bacterial infection and long-lasting antibacterial properties The effect of antimicrobial properties

Inactive Publication Date: 2007-08-08
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, sometimes the concentration of antibiotics is too low, and the effect on the prevention of BCI is not obvious; and the concentration of antibiotics is too high, which has great toxic and side effects on the human body
In addition, prolonged use of antibiotics may make bacteria resistant
[0004] (2) The method of smearing antibiotics on the surface of biological materials, the antibacterial effect of antibacterial drugs is direct, but the action period is short, the continuous stability is not strong, and there are limitations in the requirement of long-term antibacterial properties on the surface of the material
[0007] Combining antimicrobial peptide molecules to silk protein materials to make the antibacterial properties of silk protein material surfaces durable and stable is a new direction in the practical application research of medical materials, but no mature technology has yet emerged

Method used

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  • Process for preparing antibiotic-peptide modified-fibroin film material
  • Process for preparing antibiotic-peptide modified-fibroin film material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) use Na 2 CO 3 Solution (concentration 5g / L) scouring the cocoon layer 3 times to remove the soluble protein on the surface of silk protein—sericin and other impurities, 30min each time, scouring temperature 98°C, bath ratio 1:100;

[0041](2) After washing and drying, the silk protein was dissolved in 9 mol / L lithium bromide aqueous solution at 40°C. The obtained solution was dialyzed in distilled water for 3 days to remove lithium bromide to obtain an aqueous solution of silk protein, and the solution concentration (w / v) was 2%;

[0042] (3) spread the silk protein solution on a polystyrene petri dish, and dry naturally at 37°C to obtain a silk protein film;

[0043] (4) Soak the silk protein film in the mixed solution of alcohol and water for 20 minutes to make the film insoluble in water, wherein the volume percentage of alcohol is 60%;

[0044] (5) Soak and dry the silk protein film in phosphate buffer (0.1mol / L Na 2 HPO 4 , 0.5mol / L NaCl, pH6.5) soaked for...

Embodiment 2

[0049] The steps of this embodiment are all the same as in Embodiment 1, wherein:

[0050] In step (1), Na 2 CO 3 The concentration of the solution is 1g / L;

[0051] In step (2), the silk protein dissolution temperature is 30°C, the concentration of the lithium bromide aqueous solution is 8mol / L, and the concentration (w / v) of the silk protein aqueous solution is 0.1%;

[0052] In step (4), the volume percentage of alcohol is 50%, and the soaking time is 40 minutes;

[0053] In step (5), the soaking time of silk protein film in phosphate buffer solution is 45 minutes;

[0054] In step (6), the silk protein film is immersed in the mixed solution for 25 minutes, and the components and concentration of the mixed solution: 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 0.1 mg / mL, N-hydroxysuccinimide is 0.1mg / mL;

[0055] In step (7), the reaction time is 0.5 hour, and the concentration of the antimicrobial peptide in the buffer is 0.01 mg / mL.

Embodiment 3

[0057] The steps of this embodiment are all the same as in Embodiment 1, wherein:

[0058] In step (1), Na 2 CO 3 The concentration of the solution is 8g / L;

[0059] In step (2), the silk protein dissolution temperature is 70°C, the concentration of the lithium bromide aqueous solution is 10mol / L, and the concentration (w / v) of the silk protein aqueous solution is 20%;

[0060] In step (4), 100% pure alcohol is used, and the soaking time is 10 minutes;

[0061] In step (5), the soaking time of silk protein film in phosphate buffer solution is 15 minutes;

[0062] In step (6), the silk protein film is soaked in the mixed solution for 45 minutes, and the mixed solution components and concentration: 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 1mg / mL, N-hydroxysuccinimide is 1mg / mL;

[0063] In step (7), the reaction time is 1 hour, and the concentration of the antimicrobial peptide in the buffer is 1 mg / mL.

[0064] The special feature of the present inv...

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Abstract

The invention relates to a method for preparing silk fibroin film, especially a method for using covalence decoration to prepare novel antibiosis silk fibroin film. The inventive method comprises that removing foreign materials of Na2CO3 solution smelted silk fibroin, dissolving in lithium bromide water solution, drying, mixing alcohol with water, immerging in phosphate buffer solution, immerging in EDC HCl / NHS solution, immerging and washing in phosphate buffer solution, drying. The invention can adjust the density of silk fibroin, the density of antibiosis peptide solution and process covalence decoration, to control the antibiosis function. The invention has the structure and functions of natural animal silk fibroin, with surface antibiosis activity, long service life and non drug tolerance.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a method for preparing a novel antibacterial silk protein membrane material through covalent modification. Background technique [0002] In the practical application of medical materials, the infection caused by biological materials (biomaterial centered infection, BCI) is a major obstacle to the development of biomedical materials. These infections place a great burden on patients both physically and economically, and limit the wider application of bioimplant materials. Therefore, the research on the antibacterial properties of biomaterial surfaces has become a current research hotspot. In order to avoid infections caused by biological materials, the traditional method is to apply a layer of antibiotics on the surface of biomedical materials. Although this approach avoids BCI to a certain extent and promotes the clinical application of biomaterials, there are also problems ...

Claims

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Application Information

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IPC IPC(8): A61L15/46A61L15/44A61L15/32A61K38/10
Inventor 姚菊明白利强
Owner ZHEJIANG SCI-TECH UNIV
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