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Antibiotic peptide spray film forming agent and its preparing method

A technology of spraying a film-forming agent and an antimicrobial peptide, which is applied to the field of spraying an antimicrobial peptide film-forming agent for wounds or burns and its preparation, can solve the problems of easy scab debridement, poor bactericidal effect, and difficult wound healing, etc. Good affinity and air permeability, good therapeutic effect, high antibacterial activity effect

Active Publication Date: 2007-01-24
SHANGHAI HI TECH BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Infection of burn wounds can lead to difficulty in wound healing or failure of large-scale skin grafting, etc.
At present, the commonly used drugs for the treatment of burn infection have poor bactericidal effect, have no obvious effect on drug-resistant bacteria, and the wound surface is prone to crusting and debridement due to the inability to moisturize the wound, which is painful and inconvenient to administer.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of embodiment 1 antimicrobial peptide spray film-forming agent

[0034] Formula and its ratio:

[0035] Antimicrobial peptide: 10ppm

[0036] GKV-1: Lys Gly Ala Arg Lys Gly Ala Lys Arg Gln Gly Gly Lys Lys Val Ala Arg Lys Ala LeuLys Arg Ala Gly Lys

[0037] Water-soluble film-forming polymer materials: polyvinyl alcohol 2%, polyvinylpyrrolidone 5%

[0038] Humectant: Glycerin 10%

[0039] Preparation:

[0040] (1) Preparation of an antimicrobial peptide aqueous solution: adding the antimicrobial peptide to 1 / 10 of the total volume for dissolving in water, stirring and dissolving to obtain an antimicrobial peptide aqueous solution;

[0041] (2) Configure a water-soluble polymer solution: add polyvinyl alcohol to the remaining water, stir and dissolve, add polyvinylpyrrolidone, stir and dissolve to obtain a polymer solution;

[0042] (3) Preparation of the antimicrobial peptide spray film-forming agent: add the antimicrobial peptide aque...

Embodiment 2

[0043] Embodiment 2 antimicrobial peptide spray film-forming agent in vitro bactericidal test

[0044] 1. Preparation of test samples:

[0045] (1) antimicrobial peptide spray film-forming agent sample: prepared by the method of Example 1,

[0046] Antimicrobial peptide: 10ppm

[0047] GKV-2: Lys Gly Gly Arg Lys Gly Ala Lys Arg Gln Gly Gly Lys Lys Lys Leu Ala Arg Lys AlaLeu Lys Arg Ala Gly Arg

[0048] Water-soluble film-forming polymer materials: polyvinyl alcohol 2%, polyvinylpyrrolidone 5%

[0049] Humectant: Propylene Glycol 10%

[0050] (2) Control sample:

[0051] Water-soluble film-forming polymer materials: polyvinyl alcohol 2%, polyvinylpyrrolidone 5%

[0052] Humectant: Propylene Glycol 10%

[0053] 2. Test strains: Staphylococcus aureus CMCC26003, Bacillus subtilis DB430, Escherichia coli ATCC8099, Klebsiella pneumoniae CMCC46117, Pseudomonas aeruginosa CMCC10104, Candida albicans ATCC10231. (The above strains are from China Institute for the Co...

Embodiment 3

[0063] Example 3 Acute Toxicity Test in Animals

[0064] 1. Sample: antimicrobial peptide solution. Make the antimicrobial peptide injection dose reach 1mg / kg, wherein the sequences are respectively:

[0065] GKV-2: Lys Gly Gly Arg Lys Gly Ala Lys Arg Gln Gly Gly Lys Lys Leu Ala Arg Lys Ala LeuLys Arg Ala Gly Arg

[0066] GKV-3: Lys Gly Gly Arg Lys Gly Ala Lys Arg Gln Gly Gly Lys Lys Leu Ala Arg Lys Ala LeuLys

[0067] 2. Experimental animals: 60 Kunming mice, half male and half male, weighing 33.5±0.25g, were provided by the Experimental Animal Department of Fudan University.

[0068] 3. Test method: intramuscularly inject mice once a day for 7 consecutive days, and observe the toxic reaction of the animals at the maximum dose. The experimental results showed that after 7 days of intramuscular injection, the animals had no abnormal reaction and their activities were normal. After 7 days of observation, all 60 mice survived. Proved to be non-toxic.

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PUM

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Abstract

The present invention relates to antibiotic preparation for skin, and is especially a kind of antibiotic peptide spray filming preparation and its preparation process. The antibiotic peptide spray filming preparation has antibiotic peptide as the active antibiotic component, and supplementary material comprising water soluble polymer filming material and humectant. The preparation process includes dissolving the water soluble polymer material in hot water, adding the humectant and the antibiotic peptide, mixing and filling in sprayer. After being sprayed to the affected part, the antibiotic peptide spray film preparation forms one film with excellent skin affinity and air permeability in minutes for protecting skin, killing bacteria and preventing infection. The present invention has excellent curative effect on burns and infection.

Description

technical field [0001] The invention relates to an antibacterial preparation for skin, in particular to an antibacterial peptide spray film-forming agent for wounds or burns and a preparation method thereof. Background technique [0002] Burns are common wounds both in peacetime and in wartime, and are generally treated with thermal burns (including hot liquids, such as water, soup, oil, etc., steam, high-temperature gases, flames, hot metal or plastic liquids and solids, such as molten steel, steel ingots, or high-temperature burns. plastic, etc.) With the development of modern industrial and agricultural production technology, chemical and electronic burns are also increasing. Forest fires, inflammable and explosive materials burning and explosion, fires in public places and modern wars can cause batches of casualties. [0003] Infection of burn wounds can lead to difficulty in wound healing or failure of extensive skin grafting. At present, the commonly used medicines ...

Claims

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Application Information

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IPC IPC(8): A61K38/10A61K38/16A61K9/70A61K9/12A61P17/02A61P31/04
Inventor 黄青山李国栋
Owner SHANGHAI HI TECH BIOENG
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