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Wasp antibiotic peptide and preparing method and use thereof

An antibacterial peptide, wasp technology, applied in the field of biomedicine, can solve problems such as side effects and adverse reactions

Inactive Publication Date: 2009-04-08
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when the bee venom mixture is used directly for treatment, there are many side effects and adverse reactions, such as: a very small number of patients have allergies, and a small number of patients have adverse reactions such as proteinuria and kidney damage.

Method used

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  • Wasp antibiotic peptide and preparing method and use thereof
  • Wasp antibiotic peptide and preparing method and use thereof
  • Wasp antibiotic peptide and preparing method and use thereof

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Embodiment Construction

[0042] The substantive content of the present invention will be further described below with reference to the accompanying drawings, but the content of the present invention is not limited thereto.

[0043] 1. Preparation of vespa antimicrobial peptides

[0044] The bee venom collected by electric stimulation wasps was centrifuged (10000rpm, 10min) to remove the precipitate, freeze-dried and stored at low temperature.

[0045] In the first step, Sephadex G-50 gel filtration column chromatography, 300 mg of the raw material Vespa venom freeze-dried powder obtained by the above method was dissolved in 5 ml of purified water, and centrifuged at 10,000 rpm at 4°C for 10 min to remove the precipitate. SephadexG-50 gel filtration column (length 1000mm, diameter 26mm) with 0.1M Na 2 HPO 4 -NaH 2 PO 4 The pH 6.0 buffer was equilibrated sufficiently and the sample was applied to the column. Eluted with the same buffer, and collected peak III.

[0046] The second step, ion exchan...

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Abstract

The present invention belongs to the field of biomedicine technology. Wasp antibiotic peptide is a kind of single-stranded polypeptide separated from wasp venom and with molecular weight of 1316.6 dalton, isoelectric point of 8.59, and polypeptide whole sequence of NH2-IDWKGIAAMAKI-COOH. It is prepared through electrically stimulating wasp to collecting wasp venom, centrifuging to eliminate precipitate, freeze drying, chromatographic separation and purification in gel column, ion exchange column and reverse high pressure liquid phase chromatography. The wasp antibiotic peptide has powerful activity of inhibiting bacteria, fungi, viruses and tumor cell growth and the advantages of no hemolysis activity, etc. and may be used in preparing medicine for treating pathogeneic microbial infection disease and tumor.

Description

Technical field: [0001] The invention relates to a vespa antibacterial peptide and its preparation method and application, belonging to the technical field of biomedicine. Background technique: [0002] Since the discovery of penicillin, people are no longer helpless against various diseases caused by pathogenic microorganism infection, and thus developed a large number of β-lactam antibiotics, which have made great contributions to the protection of human health. However, with the widespread use of the above-mentioned "traditional antibiotics", many new problems continue to arise. Such as long-term use leads to the generation of drug-resistant strains. Sometimes it even develops to the point that some pathogenic bacteria infections have no drugs for clinical treatment. Therefore, the development of a new generation of antibacterial agents has become a top priority. Recent studies have found that polypeptide antibiotics have broad-spectrum antibacterial activity, and at t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435A61K38/17A61P31/00A61P31/04A61P35/00
Inventor 赖仞徐学清
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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