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Bacillus subtilis antibiotic peptide fengycin homologue and preparation method thereof

A technology of Bacillus subtilis and homologues, which is applied in the field of microbial fermentation and biological separation, can solve problems such as application limitations and narrow antibacterial spectrum, and achieve the effects of simple method, no toxic side effects, and high health and safety

Inactive Publication Date: 2008-03-19
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

Moreover, Nisin and Natamycin have a narrow antibacterial spectrum, so their application in food is limited

Method used

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  • Bacillus subtilis antibiotic peptide fengycin homologue and preparation method thereof
  • Bacillus subtilis antibiotic peptide fengycin homologue and preparation method thereof

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Embodiment Construction

[0028] (1) The method for microbial fermentation, separation and identification of the novel antimicrobial peptide fengycinC of the present invention is as follows:

[0029] 1. Fermentation of fengycin homologue of Bacillus subtilis fmbJ antimicrobial peptide

[0030] The Bacillus subtilis fmbJ was inoculated on the test tube slant nutrient agar medium and cultured at 37°C for 24 hours to activate the strain. The test tube oblique Bacillus subtilis fmbJ strain was inoculated into an Erlenmeyer flask containing a seed culture medium (nutrient agar medium to remove agar), and cultivated at 37°C and 130-150 rpm / min for 12-15 hours to the logarithmic growth phase. Into a concentration of 10 8 ~10 9 CFU / L seed liquid. The Bacillus subtilis fmbJ seed liquid was inoculated into a Landy fermentation medium at a concentration of 5%, and cultured at 30-33° C. and 180 rpm / min for 33-35 hours to obtain an antibacterial substance fermentation broth.

[0031] 2. Isolation of fengycin homologue ...

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Abstract

The invention relates to a bacillus subtilis fmbJ antibacterial peptide fengycin homologue and a preparation method, which belongs to the fields of microorganism fermentation and biological separation. The invention is dedicated to the microorganism fermentation and separation identification of a new antibacterial peptide fengycin homologue. The method of the microorganism fermentation and separation identification of the new antibacterial peptide fengycin homologue includes the fermentation of bacillus subtilis fmbJ, the precipitation separation of antibacterial peptide fengycin homologue acid, methanol extraction and the high-efficiency liquid chromatographic analysis of the antibacterial peptide fengycin homologue, and ESI-MS / CID analyzes and measures the molecular weight of the antibacterial peptide fengycin homologue and carries out a structural identification. The antibacterial peptide fengycin homologue can notably resist eight types of gram-negative bacteria, four types of gram-positive bacteria and six types of moulds.

Description

1. Technical Field [0001] The invention is a novel antibacterial peptide fengycin homologue of Bacillus subtilis fmbJ and its fermentation preparation, belonging to the field of microbial fermentation and biological separation. The invention relates to the microbial fermentation and separation identification of the novel antimicrobial peptide fengycinC. 2. Technical background [0002] Food is susceptible to microbial contamination in all aspects of processing, storage and transportation, packaging, sales, and consumption. How to prevent food from spoiling is a key technical issue to ensure food safety. [0003] For a long time, the food industry has been accustomed to using chemical compounds as food preservatives, such as benzoic acid and its salts, sorbic acid and its salts, and parabens. These preservatives are used in food processing. The added amount is relatively large, and it is easy to exceed the standard and cause toxic side effects to the human body. With the rapid dev...

Claims

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Application Information

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IPC IPC(8): C07K14/32C12P21/02C12R1/125
Inventor 陆兆新别小妹吕凤霞王煜
Owner NANJING AGRICULTURAL UNIVERSITY
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