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Method for fixing heparin on polylactic acid hemodialysis membrane surface

A polylactic acid, membrane surface technology, applied in the field of polymer materials and hemodialysis, to achieve the effect of less adsorption, good biocompatibility and mild conditions

Active Publication Date: 2013-09-25
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, both of the above methods use melt polymerization, which requires harsh conditions of high temperature and vacuum.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Step (1). Dopamine-coated polylactic acid film:

[0018] Weigh 0.363g of Tris and dissolve it in 600ml of deionized water to prepare a 5mmol / L Tris buffer solution, and adjust the pH to 10 with hydrochloric acid. Weigh 0.06 g of dopamine and dissolve it in the Tris buffer solution to prepare a dopamine solution with a concentration of 0.1 mg / ml, inject the dopamine solution into the inner cavity of the polylactic acid hollow fiber membrane module with a peristaltic pump, and continuously shake at 15° C. for 36 hours to obtain Polylactic acid hollow fiber membrane of polydopamine active layer; then rinsed with deionized water for 1 h to wash off unreacted dopamine.

[0019] Step (2). Covalent immobilization of heparin:

[0020] Prepare 600ml of PBS buffer solution with a pH value of 4 and a concentration of 0.1mol / L, weigh 0.15g heparin sodium and dissolve it in the PBS buffer solution to make a heparin sodium solution with a concentration of 0.25mg / ml, and place it at ...

Embodiment 2

[0022] Step (1). Dopamine-coated polylactic acid film:

[0023] Weigh 0.726g of Tris and dissolve it in 600ml of deionized water to prepare a 10mmol / L Tris buffer solution, and adjust the pH to 9 with hydrochloric acid. Weigh 0.06 g of dopamine and dissolve it in the Tris buffer solution to prepare a dopamine solution with a concentration of 1 mg / ml, inject the dopamine solution into the inner cavity of the polylactic acid hollow fiber membrane module with a peristaltic pump, and continuously shake at 30° C. for 20 hours to obtain a The polylactic acid hollow fiber membrane of the dopamine active layer; then rinsed with deionized water for 2 h to wash away unreacted dopamine.

[0024] Step (2). Covalent immobilization of heparin:

[0025] Prepare 600ml of MES buffer with a pH value of 5 and a concentration of 1mol / L, weigh 1.2g of heparin sodium and dissolve it in the MES buffer to make a heparin sodium solution with a concentration of 2mg / ml, and activate it at 5°C for 5h ,...

Embodiment 3

[0027] Step (1). Dopamine-coated polylactic acid film:

[0028] Weigh 0.871g of Tris and dissolve it in 600ml of deionized water to prepare a 12mmol / L Tris buffer solution, and adjust the pH to 8 with hydrochloric acid. Weigh 1.2 g of dopamine and dissolve it in the Tris buffer solution to prepare a dopamine solution with a concentration of 2 mg / ml, inject the dopamine solution into the inner cavity of the polylactic acid hollow fiber membrane module with a peristaltic pump, and continuously shake at 40° C. for 5 h to obtain poly The polylactic acid hollow fiber membrane of the dopamine active layer; then rinsed with deionized water for 3 h to wash away unreacted dopamine.

[0029] Step (2). Covalent immobilization of heparin:

[0030] Prepare 600ml of citric acid-sodium citrate buffer solution with a pH value of 7 and a concentration of 2mol / L, weigh 3g of heparin sodium and dissolve it in the citric acid-sodium citrate buffer solution to prepare heparin sodium with a concen...

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Abstract

The invention discloses a method for fixing heparin on polylactic acid hemodialysis membrane surface. Poly lactic acid has good biocompatibility, but has a certain of hydrophobicity, and hemodialysis membrane materials are often required to have a certain of hydrophilicity to reduce adsorption of protein and blood cells and prevent blood coagulation, so that hydrophilic modification is needed. Through use of the method of the invention, polylactic acid hemodialysis membrane surface can be modified through self-polymerization and attachment behaviors of dopamine, the heparin is covalently fixed on the membrane surface through a further reaction with heparin, and then a polylactic acid hollow fiber hematodialysis membrane having anticoagulation effect and good hydrophilicity is prepared. In the method, through utilization of the oxidation, self-polymerization, crosslinking adhesive properties of the dopamine, the heparin can be fixed on the membrane material surface. The prepared polylactic acid hollow fiber membrane has good hydrophilicity, biocompatibility, less adsorption of proteins and platelets, is not easy to cause hemolysis, and has more mild conditions than that of the prior art modification methods and obvious effects.

Description

technical field [0001] The invention belongs to the technical field of polymer materials and hemodialysis, and in particular relates to a method for fixing heparin on the surface of polylactic acid hemodialysis membrane. Background technique [0002] Polylactic acid has good biocompatibility, so it is widely used in biomedical fields, such as tissue engineering, artificial joints, surgical sutures, etc. The dry / wet induced phase inversion method is used to prepare polylactic acid solution into an asymmetric hollow fiber membrane, which can be used for hemodialysis, that is, artificial kidney. First, the good blood compatibility of polylactic acid itself can reduce complications caused by long-term dialysis and reduce mortality; second, polylactic acid is derived from open renewable resources, which can get rid of dependence on petroleum-based materials; third, polylactic acid With degradability, it is convenient for after-use disposal of disposable products such as dialyzer...

Claims

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Application Information

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IPC IPC(8): B01D71/48B01D69/08B01D67/00
Inventor 刘富薛立新高爱林
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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