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Fly maggot extractive as well as preparation method and application thereof

An extract and fly maggot technology, applied in the field of fly maggot extract and its preparation, can solve problems such as research on other components of fly maggots, stay of fly maggot extract, etc., and achieve the effects of low cost, simple operation and easy control.

Active Publication Date: 2011-03-30
浙江佰科堂生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The studies on fly maggot extracts in the above literatures all stay at the in vitro cell level, and most of them extract antimicrobial peptides and fatty acids in fly maggots, and no further research is carried out on other components in fly maggots.

Method used

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  • Fly maggot extractive as well as preparation method and application thereof
  • Fly maggot extractive as well as preparation method and application thereof
  • Fly maggot extractive as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Preparation of fly maggot extract

[0038] (1) Special breeding fly maggots

[0039] Golden fly uses animal protein 35%, 40%, 45%, brown sugar 20%, 25%, 30%, fruit 30%, 35%, 40% as food, and animal protein is maggot paste. Take the traditional Chinese medicine Epimedium 20%, 25%, 30%, plus lysine 15%, 20%, 25%, methionine 15%, 20%, 25%, vitamin B225%, 30%, 35% as the "promoting egg" "Su", use the egg tray to collect fly eggs. Use a ventilated fly maggot house, place multiple fly maggot ponds, put fly maggot bait in the maggot breeding pond, and put the above fly eggs at the same time, control the temperature at 28 -60 ℃, use the bait that has been crushed and fermented for 4-6 days Feed the fly maggots. Food selection A: Frog 15%, 23%, 30%, freshwater fish 15%, 23%, 30%, Cordyceps 0.1%, 0.15%, 0.2%, Ganoderma powder 1%, 1.5%, 2%, chicken 20%, 25%, 30%, fruit 25%, 30%, 35%, the rest is plant feed; bait selection B: pig lung 25%, 30%, 35% frog 15%, 23%, 30%, fre...

Embodiment 2

[0047] Example 2: Preparation of fly maggot extract

[0048] To 50 g of fly maggot powder prepared by special breeding fly maggots in Example 1, two times the volume of fly maggot powder was added with petroleum ether, soaked for 12 hours at room temperature, and the petroleum ether was removed to obtain defatted fly maggot powder.

[0049] The defatted fly larvae powder is ultrasonically extracted twice with 10 times the volume of the defatted fly maggot powder, each time is 10 minutes, and then filtered; the filter residue is then ultrasonically extracted twice with 8 times the volume of the filter residue. The second ultrasonic extraction for 10 minutes, filtration, and the second filtrates were combined; centrifuged at 5°C at 16000 rpm for 30 minutes, and the ultrafiltrate after ultrafiltration membrane ultrafiltration was freeze-dried to obtain brown fly maggot extract 7.4 g.

[0050] The mass percentage of water-soluble protein in the fly maggot extract determined by Lowry met...

Embodiment 3

[0054] Preparation of fly maggot extract injection

[0055] Prescription: 125g fly maggot extract prepared in Example 1

[0056] Water for injection is added to 1000mL.

[0057] Preparation method: Add water for injection to 125 g of the fly larva extract prepared in Example 1 to 1000 mL, maintain isotonic pH 7.0-7.2, filter, and fill and seal the ampoule to prepare the fly larva extract injection. The whole process is sterile, to remove heat and allergens.

[0058] 1. The fly maggot extract prepared in Example 1 was screened by the thiazole blue (MTT) method on human tumor cell lines cultured in vitro to detect the broad-spectrum anti-tumor effect of the fly maggot extract in vitro.

[0059] 1 cell line:

[0060] Human promyelocytic leukemia HL-60, human liver cancer SMMC-7721, human erythroleukemia K562, mouse leukemia P388, human lung adenocarcinoma A549, human prostate cancer PC3, human nasopharyngeal cancer CNE, human cervical cancer Hela.

[0061] 2 method

[0062] Take logari...

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Abstract

The invention discloses a fly maggot extractive containing the principal components of water-soluble proteins, and the water-soluble proteins contained in the fly maggot extractive have the mass percentage of 50-70 percent and the molecular weight of 2-16 KDa. The fly maggot extractive not only has obvious inhibiting effect on human promyelocytic leukemia HL-60 cells, human erythroleukemia K562 cells, human liver cancers SMMC-7721, mouse leukemia P388 cells, human lung adenocarcinoma A549 cells, human nasopharyngeal darcinoma CNE cells, human prostatic carcinoma PC3 cells, human cervical carcinoma HeLa in vitro, but also has outstanding inhibiting effect on mouse S180 sarcomas and mouse Heps liver cancer solid tumors, and also has the effect on enhancing the humoral immunity of organisms. The invention also discloses a preparation method of the fly maggot extractive. The preparation method is easy and convenient for operation and control and low in cost and is suitable for industrialized production.

Description

Technical field [0001] The invention relates to the field of medicinal extracts, in particular to a fly maggot extract and its preparation method and application. Background technique [0002] Housefly larvae are Chrysomia megacephala (Fab.). For medicinal purposes, there is a record in the Compendium of Materia Medica by Li Shizhen of the Ming Dynasty: "Maggots, also known as'five-grain insects','Luo fairy', and'water fairy', can treat infantile malnutrition fever and are a good medicine for treating malaria. "Fly maggots are cold and non-toxic. They are a high-quality animal protein source. Not only are they rich in protein, but the amino acid composition of the protein is also very reasonable. The mass percentage of protein in fly maggots is generally about 62%. The mass percentage of fat is generally 10%-15%. According to reports, any kind of pathogenic bacteria can survive in fly maggots for less than seven days. Therefore, scientists believe that it is very likely to hav...

Claims

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Application Information

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IPC IPC(8): A61K35/64A61P35/00A61P35/02
Inventor 彭小英马臻郑晓亮屠凌岚颜冬梅洪艳沈正荣陈建平
Owner 浙江佰科堂生物科技股份有限公司
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