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Chestnut seed alpha-1,6-glucan, preparation method for alpha-1,6-glucan, and application of alpha-1,6-glucan to anticancer drugs

A technology of dextran and chestnut, which is applied in the direction of antineoplastic drugs, drug combinations, and pharmaceutical formulations, can solve problems such as complex structures, and achieve the effects of good structural integrity, significant inhibitory activity, and excellent inhibitory activity

Inactive Publication Date: 2015-08-26
HEBEI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The polysaccharides extracted in the above studies are all composed of a variety of monosaccharides, which are heteropolysaccharides with complex structures, which only show the performance of anti-oxidation, anti-fatigue and anti-tumor activities

Method used

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  • Chestnut seed alpha-1,6-glucan, preparation method for alpha-1,6-glucan, and application of alpha-1,6-glucan to anticancer drugs
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  • Chestnut seed alpha-1,6-glucan, preparation method for alpha-1,6-glucan, and application of alpha-1,6-glucan to anticancer drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Dry and pulverize the Qianxi chestnut seed kernels (produced in Qianxi County, Hebei Province), and degrease the obtained powder with 95% ethanol for 2 days. After drying at room temperature, take 100g of chestnut seed kernel powder and add 2000mL of water, and let it stand at room temperature for 2h , stirred and extracted with cold water for 2 hours, centrifuged, and the residue was extracted twice under the same conditions, the supernatant was combined, concentrated under reduced pressure to 150mL, 3 times the volume of 95% ethanol was added under stirring, stood overnight at 4°C, and suction filtered. Dehydrated with absolute ethanol, and the obtained precipitate was freeze-dried to obtain 9.6 g of crude polysaccharide extracted from chestnut water.

[0031] Take 1 g of chestnut water-extracted crude polysaccharide and make it into 1% aqueous solution (W / V, mass volume concentration), use Sevag method to deproteinize, add an equal volume of chloroform / n-butanol mixtu...

Embodiment 2

[0042] Inhibition of cancer cell proliferation experiment

[0043] 1. Test material

[0044] Test sample: α-1,6-glucan prepared in Example 1.

[0045] Negative control substance: normal saline.

[0046] Blank control substance: PBS.

[0047] Cell lines: human cervical cancer cell HeLa, breast cancer cell MCF-7, and adriamycin-resistant breast cancer cell MCF-7 / ADR (all purchased from the Cell Bank of the Chinese Academy of Sciences).

[0048] Reagents and instruments: 0.25% trypsin (Invitrogen Company), MTT (5 mg / mL, Sigma Company); microplate reader (Bio-Rad 3550, USA).

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Abstract

The invention provides a chestnut seed alpha-1,6-glucan, a preparation method for the chestnut seed alpha-1,6-glucan, and application of the chestnut seed alpha-1,6-glucan to anticancer drugs. The structural formula of the chestnut seed alpha-1,6-glucan is [arrow 6)-alpha-Glcp(1 arrow]n, the weight average molecular weight is in the range of 1600-2400 kDa, and the average molecular weight is 2000 kDa; the chestnut seed alpha-1,6-glucan belongs to homopolysaccharides, and has obvious inhibited activity on proliferation of cervical cancer cells HeLa, breast cancer cells MCF-7 and adriamycin-resistant human breast cancer cell MCF-7 in vitro; chestnut is a rare fruit with the edible Chinese herbal function, and has no serious toxic and side effect; the chestnut seed alpha-1,6-glucan obtained according to the preparation method provided by the invention is high in sugar chain structural integrity, and is expected to be developed into anticancer drugs or assisted anticancer drugs.

Description

technical field [0001] The invention relates to a plant polysaccharide and its preparation method and application, in particular to a chestnut seed α-1,6-glucan, its preparation method and its application in antitumor drugs. Background technique [0002] Chestnut is the fruit of the genus Chestnut in the family Fagaceae. It is sweet in taste, warm in nature, and non-toxic. Clinically, chestnut can also be used to treat nausea, diarrhea, weak waist and legs, vomiting blood, blood in the stool, gold sores and other diseases. As an important component of chestnut kernels, polysaccharides have received great attention from researchers from various countries because of their wide range of biological functions and many advantages such as low toxicity and side effects. [0003] The polysaccharide components CPS-a and CPS-b were isolated from Chestnut Chestnut in Zhen’an, which have antioxidant and anti-fatigue effects (separation, purification, structural analysis and in vitro ant...

Claims

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Application Information

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IPC IPC(8): C08B37/02A61K31/716A61K36/185A61P35/00
Inventor 李红燕王应杏张金超王书香李胜辉周国强刘丹丹
Owner HEBEI UNIVERSITY
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