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Method of effectively extracting ganoderma triterpenoids and ganoderan from mythic fungus germ entity

A technology of ganoderma lucidum fruiting bodies and ganoderma polysaccharides, applied in plant raw materials, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve the problems of low extraction rate and polysaccharide content of ganoderma lucidum polysaccharides, difficult operation and high production cost, and extraction of active ingredients Low material yield and other problems, to achieve the effect of increased extraction yield, low cost, and increased yield

Active Publication Date: 2009-09-16
ZHEJIANG ESSENCE FUNGI DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the acid or alkali extraction method can improve the extraction rate, it requires high equipment and easily destroys the structure of polysaccharides, and there are also environmental pollution problems
In addition, because the fruiting body of Ganoderma lucidum is composed of cellulose, hemicellulose, lignin and other main components, the structure is tight, and the active polysaccharide active ingredients are attached to the inner side of the cell wall. The traditional hot water extraction method takes a long time and consumes a lot of energy. low yield
Chinese patent CN1181271 introduces the use of low-pressure water boiling process, the pharmacological activity of polysaccharide is higher than that of conventional water boiling, but the extraction rate of polysaccharide is low, and the content of ganoderma triterpene acid in the obtained extract is also low
However, this method requires large equipment investment, high operation difficulty and high production cost, and the extraction rate and polysaccharide content of Ganoderma lucidum are low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Take 50.0g of dried Ganoderma lucidum fruiting body powder (Shanghai Zhongling Biotechnology Co., Ltd.) into an ultrasonic extraction axe, add 2500ml of 50% ethanol, ultrasonicate for 10min at a temperature of 90°C and an ultrasonic frequency of 50KHz, and filter The obtained Ganoderma lucidum triterpene extract was spray-dried to obtain 1.38 g of Ganoderma lucidum triterpene extract with an extraction rate of 2.76%, wherein the Ganoderma lucidum triterpene content was 34.8%.

[0024] (2) Add 500ml of distilled water to the filter residue obtained in step (1), add 0.6g papain (enzyme activity 800000U / g) and 1.2g cellulase (enzyme activity 15000U / g), use 1.0mol / LNaOH and 1.0 mol / L HCl adjusted the pH of the solution to 7.0. Enzymolysis extraction was carried out at a temperature of 35° C. for 4.0 h.

[0025] (3) Enzyme inactivation at high temperature: After enzymatic hydrolysis, heat up to 100°C to inactivate the enzyme for 0.5h.

[0026] (4) Alcohol precipitation...

Embodiment 2

[0028] (1) Take 50.0g of dried Ganoderma lucidum fruiting body powder and put it into an ultrasonic extraction axe, add 500ml of 80% ethanol, ultrasonicate for 50min at a temperature of 60°C and an ultrasonic frequency of 15KHz, filter to obtain the Ganoderma lucidum triterpenoid extract and obtain it by spray drying Ganoderma lucidum triterpenoid extract 1.41g, extraction rate 2.82%, wherein Ganoderma lucidum triterpene content is 41%.

[0029] (2) Add 2500ml of distilled water to the filter residue obtained in step (1), add 1.2g papain (enzyme activity 800000U / g) and 0.6g cellulase (enzyme activity 15000U / g), use 1.0mol / LNaOH and 1.0 The pH of the solution was adjusted to 5.0 with mol / L HCl. The enzymatic extraction was performed at a temperature of 50° C. for 1.0 h.

[0030] (3) Enzyme inactivation at high temperature: After enzymatic hydrolysis, heat up to 100°C to inactivate the enzyme for 0.5h.

[0031] (4) Alcohol precipitation: After the extract is centrifuged, the s...

Embodiment 3

[0033] (1) Take 50.0g of dried Ganoderma lucidum fruiting body powder and put it into an ultrasonic extraction axe, add 250ml of 100% ethanol, ultrasonicate for 90min at a temperature of 40°C and an ultrasonic frequency of 100KHz, filter to obtain the Ganoderma lucidum triterpene extract and spray dry it 1.51 g of Ganoderma lucidum triterpenoid extract was obtained, with an extraction rate of 3.02%, wherein the content of Ganoderma lucidum triterpenoids was 48%.

[0034](2) add the distilled water of 5000ml in the filter residue of step (1) gained, add 0.3g papain (enzyme activity 800000U / g), 5.0g pectinase (enzyme activity 20000U / g) and 0.3g cellulase (enzyme activity activity 15000U / g), the pH value of the solution was adjusted to 5.0 with 1.0mol / L NaOH and 1.0mol / L HCl. The enzymatic extraction was performed at a temperature of 50° C. for 1.0 h.

[0035] (3) Enzyme inactivation at high temperature: After enzymatic hydrolysis, heat up to 100°C to inactivate the enzyme for 0...

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Abstract

The invention provides a method of effectively extracting ganoderma triterpenoids and ganoderan from mythic fungus germ entity by ultrasonic-alcohol extracting, complex enzyme-water extracting integration technology. The method is characterized by comprising the steps of: crashing the mythic fungus germ entity, using the ultrasound assisted alcohol extract (ultrasonic-alcohol extracting) to extract the ganoderma triterpenoids, then using complex enzyme assisted water extracting (complex enzyme-water extracting) to prepare ganoderan, then sponging drying to obtain ganoderma triterpenoids and ganoderan extractive. The solvents used by the method used in the invention is safe, innoxious and avoids the pollution to the environment by using chloroform, methanol and other organic solvents in the traditional method. And the invention has the features of low extraction temperature, simple device, convenient operation, low cost, fast speed, high effects and so on. Using the process to comprehensively extract triterpenoids and ganoderan active substances in glossy ganoderma can significantly increase the total active substance yield than the single extracting method.

Description

(1) Technical field [0001] The invention relates to a method for extracting ganoderma triterpenes and ganoderma polysaccharides from ganoderma lucidum fruit bodies. (2) Background technology [0002] Ganoderma lucidum (Ganoderma lucidum) is a traditional precious Chinese herbal medicine and medicinal fungus, and its main functional components are Ganoderma lucidum triterpenes and Ganoderma polysaccharides. Among them, the functions of Ganoderma lucidum polysaccharides are mainly anti-tumor, immune regulation, anti-oxidative free radicals, promoting blood circulation and removing blood stasis, and anti-aging, while Ganoderma lucidum triterpenoids have liver protection and detoxification, anti-oxidation, anti-bacterial and anti-inflammatory, anti-HIV virus, analgesia, Inhibit angiotensin-converting enzyme, inhibit cholesterol synthesis, reduce platelet aggregation, inhibit histamine release, inhibit liver tumor cells, etc. [0003] At present, the method of extracting Ganoder...

Claims

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Application Information

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IPC IPC(8): A61K36/074A61P1/16A61P7/02A61P31/00A61P31/18A61P29/00A61P35/00A61P37/02A61P39/06C12P19/04
Inventor 吴学谦吴庆其程俊文贺亮魏海龙付立忠李海波
Owner ZHEJIANG ESSENCE FUNGI DEV
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