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Method for extracting mogroside and water-soluble dietary fiber free of pesticide residue simultaneously

A technology of mogroside and dietary fiber, which is applied in the fields of chemical instruments and methods, glycoside steroids, organic chemistry, etc., can solve the problems of not being able to obtain the dietary fiber of mogroside and the low content of mogroside V in the extract, and achieve simple operation and simple equipment Effect

Active Publication Date: 2017-08-11
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method only partially removes carbendazim, a pesticide residue in the Luo Han Guo extract, and the content of mogroside V in the extract is low
In addition, this method can only obtain the Luo Han Guo extract containing mogroside V, and cannot obtain the Luo Han Guo dietary fiber

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Juicing and extraction: Wash 500kg of Luo Han Guo fresh fruit with water, squeeze the juice with a screw squeezer at room temperature, rinse the pomace with 500kg of distilled water, combine the juice and the washing solution, and obtain 750kg of the extract;

[0041] (2) Boiling: Boil the extract obtained in step (1) for 20 minutes, cool to room temperature, and centrifuge at a speed of 10,000 r / min to obtain a centrifuge;

[0042] (3) Chromatography: pass the centrifugal liquid obtained in step (2) through the basic alumina chromatography column and wood activated carbon chromatography column connected in series before and after, and collect the effluent; the mesh number of the basic alumina is 100 mesh, and the dosage 50kg, the height-diameter ratio of the alumina chromatography column is 5:1, and the flow velocity of the upper column is 0.5BV / h; the mesh number of the woody activated carbon is 100 orders, and the consumption is 60kg, and the height-diameter ratio...

Embodiment 2

[0047] (1) Juicing and extraction: wash 1500kg of Luo Han Guo fresh fruit with water, squeeze the juice with a screw squeezer at room temperature, rinse the pomace with 1350kg of distilled water, combine the juice and the washing solution, and obtain 2100kg of the extract;

[0048] (2) Boiling: Boil the extract obtained in step (1) for 30 minutes, cool to room temperature, and centrifuge at a speed of 20,000 r / min to obtain a centrifuge;

[0049] (3) Chromatography: pass the centrifugal liquid obtained in step (2) through the neutral alumina chromatography column and wood activated carbon chromatography column connected in series before and after, and collect the effluent; the mesh number of the neutral alumina is 300 mesh, and the dosage It is 180kg, and the aspect ratio of aluminum oxide chromatography column is 10:1, and the flow velocity of last column is 2BV / h; The mesh number of described wood activated carbon is 200 orders, and consumption is 150kg, and the aspect ratio ...

Embodiment 3

[0054] (1) Juicing and extraction: wash 1000kg of Luo Han Guo fresh fruit with water, squeeze the juice with a screw squeezer at room temperature, rinse the pomace with 1100kg of distilled water, combine the juice and the eluent to obtain 1600kg of extract;

[0055] (2) Boiling: Boil the extract obtained in step (1) for 15 minutes, cool to room temperature, and centrifuge at a speed of 30,000 r / min to obtain a centrifuge;

[0056] (3) Chromatography: Pass the centrifugate obtained in step (2) through the series-connected basic alumina chromatography column and coal-based activated carbon chromatography column to collect the effluent; the mesh number of the basic alumina is 200 mesh, The dosage is 200kg, the height-to-diameter ratio of the alumina chromatography column is 5:1, and the flow rate of the upper column is 1BV / h; the mesh number of the coal-based activated carbon is 400 mesh, and the dosage is 150kg, and the height-diameter ratio of the activated carbon chromatography...

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Abstract

A method for extracting mogroside and water-soluble dietary fiber free of pesticide residue simultaneously comprises the following steps that (1) momordica grosvenori is washed and juiced, pomace is rinsed, and an extraction solution is obtained; (2) boiling, cooling and centrifugation are performed, and centrifugate is obtained; (3) an effluent is collected by aluminum oxide and an activated carbon chromatographic column which are connected in series front and back; (4) ultrafiltration membrane filtration is performed, and a trapped fluid and a permeate liquid are collected respectively; (5) nanofiltration and concentration are performed: an ultrafiltration liquid is filtered by a nanofiltration membrane, the trapped fluid and the permeate liquid are collected respectively, a nanofiltration trapped fluid is concentrated and dried, and the mogroside free of pesticide residue is obtained; the nanofiltration trapped fluid and the ultrafiltration trapped fluid are combined, concentrated and dried, and the momordica grosvenori water-soluble dietary fiber free of pesticide residue are obtained; the mogroside and water-soluble dietary fiber products are free of pesticide residue, the content of glucoside V reaches 90.6%, and the yield reaches 96.3%; the purity of the water-soluble dietary fiber reaches 95%, and the yield reaches 91%; the method is simple, the operation is simple and convenient, and the method is suitable for industrialization production.

Description

technical field [0001] The invention relates to a production method of Luo Han Guo extract, in particular to a method for simultaneously extracting pesticide-free mogroside and water-soluble dietary fiber from fresh Luo Han Guo fruit. Background technique [0002] Luo Han Guo is high in sweetness and low in calories, and has health functions such as improving immunity, protecting the liver, lowering blood sugar, anti-cancer, and anti-oxidation. List of varieties of medicines", meanwhile, it is used as an ideal source of natural sweetener. [0003] At present, the development of the Luo Han Guo extract industry in China has begun to take shape, mainly based on the extraction and separation of mogrosides. However, besides mogroside, the beneficial ingredients in Luo Han Guo also have other important ingredients, such as dietary fiber. It is the current practice in the Luo Han Guo extract industry to simply use the sweet glycosides in Luo Han Guo and treat other ingredients a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00C08B37/00
CPCC07J17/005C08B37/0003
Inventor 李伟龙伟岸黄华学
Owner HUNAN HUACHENG BIOTECH
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