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Method for extracting momordica glycoside from momordica grosvenori

A technique for mogroside and Luo Han Guo extract is applied in the field of preparation of Luo Han Guo extract, which can solve the problem of high content of active ingredients, achieve the effects of high content, simplify production process and avoid loss of active ingredients

Inactive Publication Date: 2005-10-19
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved in the present invention is to overcome the defects of the existing method for extracting mogroside from Luo Han Guo, and provide a method for extracting mogroside from Luo Han Guo. The product obtained by this method has a high content of active ingredients, according to UV- -UV (ultraviolet-visible spectrophotometry) method to determine the weight content of mogroside in Luo Han Guo extract is 40-80%, or by high performance liquid chromatography (HPLC (High Performance Liquid Chromatography) method, the mogroside in Luo Han Guo extract V weight content is 20-40%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1) Pre-treatment: Squeeze the fresh Luo Han Guo fruit.

[0037] 2) Reflux extraction: Mix the raw materials and deionized water in a ratio of 1:3 and put them into the extraction tank, heat to boil and keep the temperature for 2 hours, collect the first extraction material liquid, add 3 times the amount of deionized water for the second extraction Ionized water, heat and boil for 2 hours, collect the second extraction material liquid.

[0038] 3) Concentration under reduced pressure: the extract is introduced into a concentrator for concentration under reduced pressure, the temperature is controlled below 60 degrees Celsius, and the degree of vacuum is controlled above 0.08.

[0039] 4) Centrifugation: the concentrated extract is centrifuged by a centrifuge.

[0040] 5) The concentrated and centrifuged extract directly enters the macroporous resin column;

[0041] 6) Wash the column with water until the effluent is clear;

[0042] 7) Wash the column with ethanol unti...

Embodiment 2

[0047] 1) Pre-treatment: crush the dried Luo Han Guo.

[0048]2) Reflux extraction: the raw material is mixed with ethanol with a weight concentration of 1% in a ratio of 1:12, and ultrasonic extraction is carried out. The extraction conditions are working frequency 15-60kHz, time 5-30min, extraction 3 times; after extraction, carry out decolorization resin Decolorization treatment.

[0049] 3) Concentration under reduced pressure: the extract is introduced into a concentrator for concentration under reduced pressure, the temperature is controlled below 60 degrees Celsius, the degree of vacuum is controlled above 0.08, and the volume of the extract is reduced to 1 / 4-1 / 2 of the original volume of the extract.

[0050] 4) Microfiltration to remove impurities: pump the decompressed concentrated solution into the tubular stainless steel microfiltration membrane equipment. The microfiltration membrane is a microporous membrane with a pore size of 0.05 μm to 0.3 μm to remove most of...

Embodiment 3

[0055] 1) Pre-treatment: crush the dried Luo Han Guo.

[0056] 2) Reflux extraction: the raw material is mixed with propanol with a weight concentration of 95% in a ratio of 1:2, and microwave extraction is carried out. The extraction conditions are working frequency 900-2500 MHz and time 5-30 min. Extraction, extraction 2 times; after extraction, decolorize through anion and cation columns.

[0057] 3) Concentration under reduced pressure: the extract is introduced into a concentrator for concentration under reduced pressure, the temperature is controlled below 60 degrees Celsius, the degree of vacuum is controlled above 0.08, and the volume of the extract is reduced to 1 / 4-1 / 2 of the original volume of the extract.

[0058] 4) Microfiltration to remove impurities: pump the decompressed concentrated solution into the tubular stainless steel microfiltration membrane equipment. The microfiltration membrane is a microporous membrane with a pore size of 0.08 μm to 0.12 μm to remo...

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Abstract

The process of extracting momordica glycoside from momordica grosvenori includes the following steps: extracting momordica grosvenori material with water or low carbon alcohol as solvent and in a heating, ultrasonic or microwave process; concentration; and separation and purification with chromatographic column or membrane. The extract has momordica glycoside content of 40-80 wt% or momordica glycoside V content of 20-40 wt%. The present invention has high momordica glycoside content in the product, less loss of active component, simple production process and short production period.

Description

technical field [0001] The invention relates to a preparation method of Luo Han Guo extract, in particular to a method for extracting mogroside from Luo Han Guo. Background technique [0002] Luo Han Guo (Momordicae grosvenori swingle) is a traditional health fruit with a history of 300 years of food therapy. It is sweet in taste and slightly cool in nature. It has the effects of clearing lung, relieving cough, and promoting gastrointestinal function. It can treat acute and chronic bronchitis, acute and chronic tonsillitis, acute gastritis, and constipation. [0003] Research on the composition of Luo Han Guo shows that: [0004] 1. Luo Han Guo contains 1% strong sweet substance of triterpenoid glycoside S-5, which is 300 times sweeter than sucrose, much sweeter than stevioside and glycyrrhizin, and has no bitter smell of stevioside. This strong sweet substance tastes sweet and pure, has no peculiar smell, has a strong aftertaste, and has good thermal stability. It is stab...

Claims

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Application Information

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IPC IPC(8): C07J9/00
Inventor 秦本军宋云飞汤凌志季永贤
Owner GUILIN NATURAL INGREDIENTS CORP
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