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Repairing method of microorganism in contaminated soil of chromium slag storage yard

A technology for microbial remediation and contaminated soil, applied in the restoration of contaminated soil, etc., can solve the problems of a large number of chemical reagents, uneconomical, high energy consumption, etc., and achieve the effect of high removal efficiency, low cost, and simple operation

Inactive Publication Date: 2009-12-16
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these traditional methods require high energy consumption or a large amount of chemical reagents, which are uneconomical or cause secondary pollution, so they have not been widely used in engineering

Method used

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  • Repairing method of microorganism in contaminated soil of chromium slag storage yard
  • Repairing method of microorganism in contaminated soil of chromium slag storage yard
  • Repairing method of microorganism in contaminated soil of chromium slag storage yard

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Isolation, purification and domestication of chromium-reducing bacteria of the present invention

[0018] Collect the soil under the chromium slag dump site of Hunan Ferroalloy Factory, weigh 10g of chromium slag soil in a 150mL sterilized Erlenmeyer flask, add 10mL sterilized liquid medium (5g / L sodium lactate, 5g / L yeast extract and 2g / L Sodium chloride, the rest is water, adjust pH=9.5~9.8 with 5mol / L NaOH;), cultivate in 30°C incubator for 4 days. Then take 1mL mud and dilute to 10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 , with a pipette from 10 -6 、10 -7 and 10 -8 Take 0.1mL of the diluent and place it in a solution containing 250mg / L Cr(VI) (in K 2 Cr 2 o 7 Solution as chromium source) solid medium, solid medium consists of: 5g / L sodium lactate, 5g / L yeast extract and 2g / L sodium chloride, 15g / L agar. Spread evenly with a triangular stick, and place the plate upside down in a 30°C incubator for 4 days. Bacterial colonies of different forms...

Embodiment 2

[0021] Example 2: Remediation Test of Contaminated Soil in Chromium Slag Stockyard

[0022] Weigh 10g of the soil under the chromium slag dump of Hunan Ferroalloy Factory, put it in a 150mL Erlenmeyer flask filled with 10mL of liquid medium, and place it in an incubator at 30°C for 10 days. On the 5th and 10th days, the cultured soil samples were randomly taken, poured out, spread the soil samples dry, air-dried at room temperature, ground, and passed through a 10-mesh sieve to determine the total hexavalent chromium, water-soluble hexavalent chromium, and exchangeable chromium in the soil. Contents of valent chromium, carbonate-bound hexavalent chromium, iron-manganese bound hexavalent chromium, organic bound hexavalent chromium and residual hexavalent chromium.

[0023] On the fourth day after cultivation, the removal rate of total hexavalent chromium in the soil reached 92%, and after 10 days, the removal rate reached 98%; after 5 days of cultivation, the water-soluble hexa...

Embodiment 3

[0024] Embodiment 3: simulated chromium-contaminated soil remediation test

[0025] Take uncontaminated soil, air-dry, grind, and pass through a 10-mesh sieve. Potassium dichromate solution was added to the sieved soil to prepare contaminated soil containing Cr(VI) of 210mg / kg, 360mg / kg, 410mg / kg, 610mg / kg, and 1010mg / kg respectively. Weigh 20g of the above soil, add the acclimatized bacterial culture medium (10% inoculum) according to the soil-liquid mass-volume ratio (g:mL) of 1:2, the pH of the medium is 9.5, and place it in an incubator at 30°C The concentration of Cr(VI) in the soil was measured regularly. After 13 days, the removal rates of Cr(VI) were 40%, 63%, 96%, 100% and 100% (as attached Figure 4 shown).

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Abstract

The invention discloses a repairing method of microorganism in contaminated soil of chromium slag storage yard, comprising the steps of selecting Pannonibacter phragmitetus BB single bacterium, dropping the single bacterium in a liquid culture medium containing Cr (VI) of 50-250mg / L for culturing until Cr (VI) is not detected in the culture solution, selecting strains in a bottle for transferring, gradually improving the Cr (VI) concentration for gradient domestication, adding the domesticated Pannonibacter phragmitetus BB lysate in the contaminated soil, and culturing for 4-5 days at a temperature below 30 DEG C, so as to repair the soil, wherein the amount of the added lysate is that the mass-volume ratio (g: mL) of the soil to the lysate is 1: 2. The invention treats and repairs hexavalent chromium-contaminated soil through the strains with hexavalent chromium reducing capacity, the operation is simple and feasible, the cost is low, and the method is an environment-friendly treatment method.

Description

technical field [0001] The invention relates to the field of microbial remediation of polluted soil, in particular to the microbial treatment of hexavalent chromium pollution. Background technique [0002] Chromium is an increasingly important metal pollutant in the environment due to ore smelting, ferroalloy production, pigment making, leather processing, and coal and petroleum fuel combustion. China is a big producer of chromium salts. Since metal smelting and chemical industries have piled chromium slag into the soil for more than 30 years, the stockpile has reached 6 million tons, and the annual rate of 200,000 to 300,000 tons continues to be discharged. Due to the lack of appropriate treatment measures in most chromium salt production industries, a large amount of chromium-containing waste residue has become a serious pollutant of the soil. Especially the ferroalloy production industry is one of the most important sources of soil chromium pollution. According to repor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
Inventor 柴立元杨志辉彭兵王云燕闵小波王海鹰黄顺红许友泽王兵苏长青
Owner CENT SOUTH UNIV
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