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Microbial agent for treating ammonia-containing waste water and preparation method thereof

A microbial agent and wastewater treatment technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unsuitable industrial wastewater, low scale production efficiency, and expensive products, and achieve enhanced durability Receptivity and biochemical activity, long storage time, good performance

Active Publication Date: 2010-12-01
CHINA PETROLEUM & CHEM CORP +1
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AI Technical Summary

Problems solved by technology

[0006] Although there are some technologies on the cultivation of nitrifying bacteria at present, the large-scale production efficiency is low, the product price is relatively expensive, and can only be used to treat ammonia nitrogen wastewater with low concentration such as lower than 500mg / L, such as the nitrification disclosed in Chinese patent CN1509991A The bacterial agent involves six bacterial strains including Nitrobacter hamburger, Nitrobacter victorii and Saccharomyces cerevisiae. The role of ammonia nitrogen, used alone or adapted to the corresponding bioreactor, can be used for the removal of ammonia nitrogen and COD in mariculture and industrial circulation seawater high-density culture, not suitable for the treatment of industrial wastewater with low COD and high ammonia nitrogen concentration

Method used

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Examples

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Effect test

Embodiment 1

[0040] Embodiment 1 directional domestication method improves the content of nitrous acid bacteria strain

[0041] Before directional domestication, the method provided in Chinese patent 200710010383.0 is used to enrich the nitrifying bacteria to obtain activated sludge containing high concentrations of nitrous bacteria and nitric acid bacteria. Then select the directional acclimatization of the enriched bacteria source under high ammonia nitrogen concentration and high pH value. The amount of activated sludge inoculum is 1.0g / L according to the MLSS after inoculation, and the ammonia-containing wastewater with a self-prepared concentration of 300mg / L (COD value 30mg / L) is directly used as the reactor feed water, the pH is controlled at 7.9, and the temperature is controlled. The concentration of dissolved oxygen at 30°C is about 2.0-3.0 mg / L. Water is fed intermittently first, and ventilation is stopped after 24 hours of reaction. After natural settlement, the supernatant is...

Embodiment 2

[0042] The tertiary cultivation of embodiment 2 composite microorganisms

[0043] The tertiary culture was carried out with the microbial flora obtained in Example 1 as the inoculum. First, the inoculum was inoculated into a 10L reactor for primary scale-up cultivation of the bacteria. The initial biomass was 2g, the concentration of ammonia nitrogen in the culture solution was 1000mg / L, the COD was 46mg / L, and the volume of the initial culture solution was 5L; Ammonium chloride solution with an ammonia nitrogen concentration of 10-15g / L was added every 24 to 48 hours. After the ammonium salt was supplemented, the ammonia nitrogen concentration in the culture solution was higher than 700mg / L. After 7 days of cultivation, the MLSS was 1.5g / L. Since then, the removal rate of ammonia nitrogen has decreased, and the aeration was stopped. After natural sedimentation, the supernatant was removed, and the bacteria were transferred to a 60L reactor for secondary cultivation. The conc...

Embodiment 3

[0044] The preparation of embodiment 3 microbial bacterial agents

[0045] The microorganisms cultured in Example 2 were naturally settled to obtain a bacterial suspension with a water content of 50%, and then a nutrient solution twice the volume of the bacterial suspension was added. NH per liter of nutrient solution 4 + Fe 2+ , Mg 2+ 、K + , Ca 2+The molar ratio of these five cations is 2000:5:20:20:15, then add 2.5% dimethyl sulfoxide in the volume of the bacterial suspension, then pack into 500ml plastic bottles, and store at -70°C spare. In the microbial agent, Nitrobacter sp. accounts for 9%, Nitrosomonas sp. accounts for 82%, and Denitrifying bacteria (Denitrobacter sp.) accounts for 7%.

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Abstract

The invention discloses a microbial agent for treating ammonia-containing waste water and a preparation method thereof. The microbial agent for treating ammonia-containing waste water mainly comprises three bacterial strains of nitrobacter sp, nitrosobacteria nitrosomonas sp., denitrobacter sp., and the contents of the three bacterial strains can be regulated or proportioned according to specificcultivation conditions and product requirements through methods of oriented domestication, and the like. The cultivation method comprises the following steps of: enriching a bacterial source, purifying and amplifying stage by stage for cultivating. The microbial agent can be used for oriented domestication according to a waste water treatment result so as to improve the content of a target bacterial strain. The cultivation method has the advantages of high cultivation efficiency, large production capacity, and the like and is suitable for commercially producing complex microorganisms for treating the ammonia-containing waste water in a large scale. The obtained microbial agent can effectively treat low-COD (Chemical Oxygen Demand) and high-ammonia-nitrogen waste water, such as waste water of catalyst production, and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agents, and in particular relates to a sewage treatment microbial bacterial agent and a preparation method thereof, which can realize the industrialization and commercialization of microorganisms used for treating ammonia-containing wastewater. Background technique [0002] Biological denitrification of wastewater includes two processes of nitrification and denitrification. The microorganisms involved in nitrification are mainly nitrifying bacteria, which are typical autotrophic bacteria that obtain energy from the process of biological oxidation of ammonia nitrogen to nitrate nitrogen. Generally, inorganic carbon is used as the Carbon source, so the metabolism time is long, the growth is relatively slow, it belongs to Gram-negative bacteria, the content of peptidoglycan in the cell wall is low, the content of protein and fat is high, and it is sensitive to environmental changes. Recep...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/01C02F3/34C02F101/16C12R1/01
Inventor 高会杰黎元生许谦佟明友张全李志瑞唐似茵
Owner CHINA PETROLEUM & CHEM CORP
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