Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of mucus protein peptide of andrias davidianus

A mucin and giant salamander technology, which is applied in the field of preparation of giant salamander mucin peptides, can solve problems such as rare protein, and achieve the effects of retaining effective ingredients, good color and luster, and retaining biological activity.

Inactive Publication Date: 2015-10-21
CHONGQING KUIFENG FOOD CO LTD
View PDF1 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are relatively few reports on the extraction and development of proteins in the mucus of giant salamanders

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1) Collect 2 kg of giant salamander mucus by electric shock stimulation method, freeze-dry and dehydrate, grind and pulverize to obtain mucus freeze-dried powder;

[0018] 2) Add 100 times the mass of the obtained mucus freeze-dried powder to distilled water, then add alkaline protease with 20% mass of the mucus freeze-dried powder, adjust the pH to 9, enzymatically hydrolyze at 400°C for 3 hours, and then boil the mixture 1min, cooling to 19~25℃;

[0019] 3) Centrifuge the mixed liquid treated in step 2) at 3000r / min for 20min, take the clear liquid, adjust the pH value of the clear liquid to 4, and add 1% activated carbon of the clear liquid weight under the temperature condition of 45°C, Stir for 45min to decolorize and deodorize, then centrifuge at 4500r / min for 10min, pass through a 0.45μm filter membrane to obtain the filtrate;

[0020] 4) Concentrate the treated product in step 3) by rotary evaporation at 30°C to 1 / 7 of the original volume, seal the concentrate ...

Embodiment 2

[0022] 1) Collect 2 kg of giant salamander mucus by electric shock stimulation method, freeze-dry and dehydrate, grind and pulverize to obtain mucus freeze-dried powder;

[0023] 2) Add 120 times the mass of the obtained mucus freeze-dried powder to distilled water, then add alkaline protease with 10% mass of the mucus freeze-dried powder, adjust the pH to 9.5, enzymatically hydrolyze at 60°C for 1 hour, and then boil the mixture 2min, cooling to 19~25℃;

[0024] 3) centrifuge the mixed liquid processed in step 2) at 4500r / min for 10min, take the clear liquid, adjust the pH value of the clear liquid to 4, and add 2% activated carbon of the clear liquid mass under the temperature condition of 40°C, Stir for 45 minutes for decolorization and deodorization, then centrifuge for 15 minutes at 3000r / min, pass through a 0.45μm filter membrane to obtain the filtrate;

[0025] 4) Concentrate the filtrate treated in step 3) by rotary evaporation at 60°C to 1 / 8 of its original volume, s...

Embodiment 3

[0027] 1) The giant salamander mucus was collected by electric shock stimulation method, freeze-dried and dehydrated, then ground and pulverized to obtain the mucus freeze-dried powder;

[0028] 2) Add 50 times the mass of the obtained mucus freeze-dried powder to distilled water, then add alkaline protease with 10% mass of the mucus freeze-dried powder, adjust the pH to 9, enzymatically hydrolyze at 50°C for 2 hours, and then boil the mixture 1min, cooling to 19~25℃;

[0029] 3) Centrifuge the mixed liquid processed in step 2) for 150 min at 4000 r / min, take the clear liquid, adjust the pH value of the clear liquid to 4.5, and add 1.5% activated carbon of the clear liquid mass under the temperature condition of 43°C, Stir for 50 minutes for decolorization and deodorization, then centrifuge for 10 minutes at 4000r / min, pass through a 0.45μm filter membrane to obtain the filtrate;

[0030] 4) Concentrate the filtrate treated in step 3) by rotary evaporation at 40°C to 1 / 7 of t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of mucus protein peptide of andrias davidianus. The preparation method comprises the steps that 1, mucus of the andrias davidianus is collected through an electric shock stimulus method, and freeze-dried to obtain freeze-dried mucus powder; 2, the obtained freeze-dried mucus powder is added to distilled water with the mass of 50-120 times, then alkaline protease which is 10 percent-30 percent of the mass of the freeze-dried mucus powder is added, pH is adjusted to 9-9.5, enzymolysis is carried out for 1-3 h under the temperature condition of 40 DEG C-60 DEG C, and then mixed liquid is boiled for 1-2 min and cooled to 19 DEG C-25 DEG C; 3, the mixed liquid treated in the step 2 is centrifuged for 10-20 min under the condition of 3,000-4,500 r / min, clear liquid is taken, the pH value of the clear liquid is adjusted to 4-5, under the temperature condition of 40 DEG C-45 DEG C, activated carbon of one percent to two percent is added, stirring is carried out for 45-60 min for decolorizing and deordorizing, and then under the condition of 3,000-4,500 r / min, centrifugation is carried out for 10-15 min, and filter liquor is obtained through a filter film of 0.45 micrometer; 4, the filter liquor treated in the step 3 is concentrated to 1 / 8 -1 / 7 of an original sample under the temperature condition of 30 DEG C-60 DEG C in a rotary evaporation mode, concentrated liquor is sealed with a plastic bag, sterilization is carried out for 1-5 min under the conditions that temperature ranges from 19 DEG C to 25 DEG C and pressure ranges from 150 MPa to 250 MPa, and vacuum freeze-drying and smashing are carried out in clear environment to obtain mucus protein peptide powder.

Description

technical field [0001] The invention belongs to the field of giant salamander deep processing and relates to a preparation method of giant salamander mucus protein peptide Background technique [0002] The giant salamander (Andrias davidianus) belongs to Amphibians, Cauraceae, and Cryptobranchidae. It is a rare and special product unique to my country. Since the 1990s, Hunan, Hubei, Shaanxi and Guangxi have established a large number of giant salamander domestication and breeding enterprises. In Hunan Province alone, there are about 35 giant salamander domestication and breeding enterprises, with an annual reproductive capacity of 40,000 to 50,000 fish. With the expansion of breeding scale, the intensive processing and utilization of giant salamander resources has become an urgent issue related to the sustainable development of giant salamander industry. Up to now, there is no deep processing and utilization technology of giant salamander with significant economic benefits...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P21/06C07K1/34
Inventor 王莹陈兵魏泓刘良忠商海涛苟强周明辉金伟
Owner CHONGQING KUIFENG FOOD CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com