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Cordyceps militaris strain for producing cordycepin with high yield

A technology for Cordyceps militaris and cordycepin, which is applied in the field of bioengineering, can solve the problems of long fermentation period, research on Cordyceps militaris strains, and cannot be applied to industrialized production, and achieves the effects of high yield and short fermentation time.

Inactive Publication Date: 2013-01-16
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] None of these reports carried out follow-up further research on the Cordyceps militaris strains that can produce cordycepin at higher temperatures, and the fermentation period is long, which cannot be applied to industrial production

Method used

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  • Cordyceps militaris strain for producing cordycepin with high yield
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  • Cordyceps militaris strain for producing cordycepin with high yield

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Effect test

Embodiment 1

[0041] Determination of the optimum temperature

[0042] will strain Cordyceps militaris JN168 was inoculated on PDA slant medium, cultured under light at 30°C for 7 days, and the spores were washed with sterile water to make 10 spores 6 times the spore suspension. Take the prepared spore suspension, put it into the PDA seed medium with 5% inoculum amount, and cultivate it in shake flask for 3 days at 30°C and 200 rpm to obtain the liquid mother seed; put it into the fermentation medium with 5% inoculum amount, and after 5 days The fermentation is over. The composition of the seed medium and the fermentation medium are both glucose 40g / L, yeast extract 10g / L, and magnesium sulfate 0.5g / L. The optimal temperature of cordycepin production by Cordyceps militaris was investigated by single factor, the fermentation temperature was adjusted to 15°C, 20°C, 25°C, 30°C, 35°C respectively, and the concentration of cordycepin was measured. Depend on figure 1 According to the data, ...

Embodiment 2

[0044] Determination of optimum pH

[0045] will strain Cordyceps militaris JN168 was inoculated on PDA slant medium, cultured under light at 30°C for 7 days, and the spores were washed with sterile water to make 10 spores 6 times the spore suspension. Take the prepared spore suspension, connect it to the PDA seed medium with 5% inoculum amount, and culture it in a shaker flask for 3 days at 30°C and 200 rpm to obtain the liquid mother seed; insert it into the fermentation medium with 5% inoculum amount, 30°C, the fermentation ended after 5 days. The composition of seed culture medium and fermentation medium is glucose 40g / L, yeast extract 10g / L, magnesium sulfate 0.5g / L. The optimal pH of cordycepin produced by Cordyceps militaris was investigated by single factor, the fermentation pH was adjusted to 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, and the concentration of cordycepin was measured. Depend on figure 2 According to the data, the optimum pH for producing cordycepin from ...

Embodiment 3

[0047] Determination of the best carbon source

[0048] will strain Cordyceps militaris JN168 was inoculated on PDA slant medium, cultured under light at 30°C for 7 days, and the spores were washed with sterile water to make 10 spores 6 times the spore suspension. Take the prepared spore suspension, connect it to the PDA seed medium with 5% inoculum amount, and culture it in a shaker flask for 3 days at 30°C and 200 rpm to obtain the liquid mother seed; insert it into the fermentation medium with 5% inoculum amount, 30°C, the fermentation ended after 5 days. The composition of the seed medium and the fermentation medium is 40g / L of glucose, 10g / L of yeast extract, 0.5g / L of magnesium sulfate, and the pH is 7.0. Using single factor to investigate the best carbon source of cordycepin produced by Cordyceps militaris, using fructose, maltose, sucrose, lactose, D-galactose, soluble starch to replace glucose in the basic fermentation medium for liquid fermentation, and measuring ...

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Abstract

The invention discloses a cordyceps militaris strain for producing cordycepin with high yield and belongs to the technical field of bioengineering. The cordyceps militaris strain is named as cordyceps militaris JN168 by classification and is preserved with CCTCC No: M2011333 inChinaCenter for Type Culture Collection. The cordyceps militaris strain which is stable in character is obtained by isolation and screening of wild cordyceps militaris sporocarps collected from Antu, Jilin Province. The cordyceps militaris strain is capable of producing the cordycepin by liquid-state fermentation at the fermentation temperature of 30 DEG C, and the final concentration of cordycepin can be 6g / L after fermentation for 5 days.

Description

technical field [0001] A strain of Cordyceps militaris belongs to the technical field of bioengineering. The invention relates to a Cordyceps militaris strain capable of high-yielding cordycepin at a relatively high fermentation temperature of 30°C. The color of its colonies and fruit bodies are both yellow, and the strain is of great significance to the industrial production of cordycepin. Background technique [0002] Cordyceps militaris is one of the precious Chinese medicinal materials in my country, which has high medicinal value and nutritional value. The main chemical composition of Cordyceps militaris is very similar to wild Cordyceps sinensis, mainly including nucleosides (such as cordycepin, adenosine, etc.), Cordyceps polysaccharides, cordycepic acid, sterols, superoxide dismutase and amino acids. Modern studies have shown that there are many active ingredients in Cordyceps militaris, especially cordycepin has a variety of biological activities. Cordycepin is an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/40C12R1/645
Inventor 蔡宇杰丁彦蕊岳翠翠陈涛金大勇朱建航廖祥儒张大兵李枝玲罗军侠
Owner JIANGNAN UNIV
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