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Preparation of gold needle mushroom polysaccharide

A technology of Flammulina velutipes polysaccharides and seeds, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of easy pollution, large equipment investment, and prolonged fermentation time

Active Publication Date: 2009-02-25
JIANGSU HENGRUI ZELANG BIO ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses glucose as the main carbon source, which affects the yield of exopolysaccharides. In order to prolong the fermentation time, multi-stage batch fermentation is adopted, which is cumbersome to operate, requires a large investment in equipment, and is easy to cause pollution.

Method used

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  • Preparation of gold needle mushroom polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0118] (1) Activation of slant strains:

[0119] The strains of Flammulina velutipes were grafted onto the slant of potato juice comprehensive medium, and cultured at a constant temperature of 24°C for 12 days.

[0120] The selected potato comprehensive medium contains the following substances in weight and volume percentages: 1.0% glucose, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 20% potato juice, and 2.0% agar.

[0121] (2) Shake flask seed culture:

[0122] Put 20ml of seed medium in a 50ml triangular flask, sterilize it, connect it to the slant mother seed after cooling, shake it, and co-cultivate it for 5 days;

[0123] Seed medium formula: 3% corn flour, 1% sucrose, 1% bran, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.005% each of vitamin B1 and vitamin B2.

[0124] The conditions for seed culture in shake flasks are as follows: at 25° C., the rotation speed of the shaker is 200 r / min, and the initial pH value of the culture sol...

Embodiment 2

[0135] (1) Activation of slant bacteria:

[0136] The strains of Flammulina velutipes were grafted onto the slant of potato juice comprehensive medium, and cultured at a constant temperature of 24°C for 10 days.

[0137] The selected potato comprehensive medium contains the following substances in weight and volume percentages: 1.0% glucose, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 20% potato juice, and 2.0% agar.

[0138] (2) Shake flask seed culture:

[0139] Put 20ml of seed culture medium in a 50ml triangular flask, sterilize it, connect it to the slant mother seed after cooling, shake it, and co-cultivate it for 4 days;

[0140] Seed medium formula: 3% corn flour, 1% sucrose, 0.5% peptone, 2% soybean cake powder, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.005% each of vitamin B1 and vitamin B2.

[0141] The conditions for seed culture in shake flasks are as follows: at 25° C., the rotation speed of the shaker is 200 r / min, and t...

Embodiment 3

[0152] (1) Activation of slant bacteria:

[0153] The strains of Flammulina velutipes were grafted onto the slant of potato juice comprehensive medium, and cultured at a constant temperature of 24°C for 14 days.

[0154] The selected potato comprehensive medium contains the following substances in weight and volume percentages: 1.0% glucose, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 20% potato juice, and 2.0% agar.

[0155] (2) Shake flask seed culture:

[0156] Put 20ml of seed culture medium in a 50ml triangular flask, sterilize it, connect it to the slant mother seed after cooling, shake it, and co-cultivate it for 6 days;

[0157] Seed medium formula: 3% corn flour, 1% sucrose, 0.5% amino acid, 2% yeast extract, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.005% each of vitamin B1 and vitamin B2.

[0158] The conditions for seed culture in shake flasks are as follows: at 25° C., the rotation speed of the shaker is 200 r / min, and the ...

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Abstract

The invention relates to a method for preparing flammulina velutipes polysaccharide with simple operation and low equipment investment, which comprises the following process procedures: activating a slant strain, shaking culture of seeds, submerged fermentation in liquid, continuous fermentation, separation by an ultrafiltration membrane, condensation and alcohol precipitation. The method for preparing the flammulina velutipes polysaccharide has the advantages of continuous fermentation in a fermenter, prolonging the fermentation time, simplifying the operation flow and improving the polysaccharide yield.

Description

technical field [0001] The invention relates to a preparation method of Flammulina velutipes polysaccharides, in particular to a method for producing Flammulina velutipes polysaccharides by a fermentation method. Background technique [0002] Flammulina velutipes [Flainmulina velutipes (Fr.) Singer] has another name called mushroom, hair handle money fungus, is one of the famous health-care edible fungus at all times and in all over the world. Flammulina velutipes polysaccharide is a kind of polysaccharide extracted, separated and purified from Flammulina velutipes, which can promote the synthesis of protein and nucleic acid and improve the biological immunity of the body. At present, the polysaccharides of Flammulina velutipes can be extracted from the fruit body, mycelium and submerged fermentation broth. The artificial cultivation period of Flammulina velutipes is long, and the process of extracting polysaccharides from it is not easy to control, and the cost is high and ...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12N1/14C12R1/645
Inventor 刘东锋范淦彬杨成东
Owner JIANGSU HENGRUI ZELANG BIO ENG CO LTD
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