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Method for producing docosahexaenoic acid by fermentation tank substrate feeding

A docosahexaenoic acid and fermenter technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve problems such as unfavorable synthesis

Inactive Publication Date: 2016-12-07
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yokochi et al. (Yokochi T.et al.1998) optimized the culture conditions of Schizochytrium limacinum SR21 bacteria, and found that glucose, fructose or glycerol were used as carbon sources, and the cells could grow better and achieve higher DHA output, and DHA accounts for 30% of the total fat, and the other unsaturated acids produced are only DPA and 1% EPA and AA; and it is also found that maltose and starch are obviously not conducive to the synthesis of DHA

Method used

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  • Method for producing docosahexaenoic acid by fermentation tank substrate feeding
  • Method for producing docosahexaenoic acid by fermentation tank substrate feeding
  • Method for producing docosahexaenoic acid by fermentation tank substrate feeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Activation of strains: The strain Schizochytrium sp. LU310 preserved at -70°C was transferred to the plate medium, and cultured at 28°C for 24-48 hours to obtain activated strains and observe the colony morphology. The composition of the plate medium is (g / L): glucose 30, yeast powder 10, Na 2 SO 4 12. MgSO 4 2. KH 2 PO 4 1, (NH 4 ) 2 SO 4 1, K 2 SO 4 0.65, KCl 0.5, CaCl 2 .2H 2 O0.17, agar 15, pH adjusted to 6.5.

[0030] 2) Primary seed culture: select the plate colonies in step 1) with good morphology, pick them with an inoculating loop and insert them into a 250 mL flat-bottomed conical flask containing 50 mL of seed medium, and cultivate at 28° C. and 200 rpm for 24- 48h; the composition of seed medium is (g / L): glucose 30, yeast powder 10, Na 2 SO 4 12. MgSO 4 2. KH 2 PO 4 1, (NH 4 ) 2 SO 4 1, K 2 SO 4 0.65, KCl0.5, CaCl 2 .2H 2 O 0.17, the pH was adjusted to 6.5, and the first-class seed culture solution was obtained.

[0031]...

Embodiment 2

[0037]1) Activation of strains: The strain Schizochytrium sp. LU310 preserved at -70°C was transferred to the plate medium, and cultured at 28°C for 24-48 hours to obtain activated strains and observe the colony morphology. The composition of the plate medium is (g / L): glucose 30, yeast powder 10, Na 2 SO 4 12. MgSO 4 2. KH 2 PO 4 1, (NH 4 ) 2 SO 4 1, K 2 SO 4 0.65, KCl 0.5, CaCl 2 .2H 2 O0.17, agar 15, pH adjusted to 6.5.

[0038] 2) Primary seed culture: select the plate colonies in step 1) with good morphology, pick them with an inoculating loop and insert them into a 250 mL flat-bottomed conical flask containing 50 mL of seed medium, and cultivate at 28° C. and 200 rpm for 24- 48h; the composition of seed medium is (g / L): glucose 30, yeast powder 10, Na 2 SO 4 12. MgSO 4 2. KH 2 PO 4 1, (NH 4 ) 2 SO 4 1, K 2 SO 4 0.65, KCl0.5, CaCl 2 .2H 2 O 0.17, the pH was adjusted to 6.5, and the first-class seed culture solution was obtained.

[0039] ...

Embodiment 3

[0045] 1) Activation of strains: The strain Schizochytrium sp. LU310 preserved at -70°C was transferred to the plate medium, and cultured at 28°C for 24-48 hours to obtain activated strains and observe the colony morphology. The composition of the plate medium is (g / L): glucose 30, yeast powder 10, Na 2 SO 4 12. MgSO 4 2. KH 2 PO 4 1, (NH 4 ) 2 SO 4 1, K 2 SO 4 0.65, KCl 0.5, CaCl 2 .2H 2 O0.17, agar 15, pH adjusted to 6.5.

[0046] 2) Primary seed culture: select the plate colonies in step 1) with good morphology, pick them with an inoculating loop and insert them into a 250 mL flat-bottomed conical flask containing 50 mL of seed medium, and cultivate at 28° C. and 200 rpm for 24- 48h; the composition of seed medium is (g / L): glucose 30, yeast powder 10, Na 2 SO 4 12. MgSO 4 2. KH 2 PO 4 1, (NH 4 ) 2 SO 4 1, K 2 SO 4 0.65, KCl0.5, CaCl 2 .2H 2 O 0.17, the pH was adjusted to 6.5, and the first-class seed culture solution was obtained.

[0047]...

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Abstract

The invention discloses a method for producing docosahexaenoic acid by fermentation tank substrate feeding, and relates to docosahexaenoic acid. The method comprises the following steps: transferring a strain into a plate medium for cultivation to obtain an activated strain, wherein the strain is Schizochytrium sp. LU310, with the preservation number of CGMCC No. 12528; inoculating the obtained activated strain in a plate bacterial colony into a conical flask with a seed culture medium for cultivation; inoculating an obtained fermented culture solution into a fermentation culture medium for cultivation, automatically controlling the pH of the fermented solution by feeding 2M of NaOH or 1M of citric acid, sampling every 4 h to measure the glucose concentration, and sampling every 12 h to measure the biomass, the yield of grease and the yield of DHA.

Description

technical field [0001] The invention relates to docosahexaenoic acid, in particular to a method for producing docosahexaenoic acid by stream addition to the bottom of a fermenter. Background technique [0002] Docosahexaenoic acid (DHA) is one of the main highly unsaturated fatty acids and belongs to the ω-3 series of polyunsaturated fatty acids. Its molecular formula is C 22 H 32 O 2 , with a relative molecular mass of 328.49, also known as "brain gold", which has many important physiological functions such as promoting infant brain development, protecting eyesight, preventing and treating cardiovascular diseases (Crawford P.1987; Birch EE.et al .2002; Uauy R. et al. 2006), it has received extensive attention as a functional factor added to health food and functional food (Agren JJ. et al. 1996; Smith Jr SC. et al. 2006). Due to its wide range of uses, its global market demand has also greatly increased. At present, fish oil is the main source of commercial DHA, especi...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/64C12R1/645
CPCC12P7/6472C12N1/145C12R2001/645
Inventor 凌雪萍潘雪珊卢英华郭静敬科举姚传义陈翠雪
Owner XIAMEN UNIV
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