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Method for increasing added values of raw materials of schizochytrium sp. solid-state fermented feed

A technology for Schizochytrium and feed raw materials, applied in the field of fermentation, can solve the problems of complex reaction device, complex culture medium and high energy consumption, achieve broad market prospects, improve nutritional value, and reduce production costs and energy consumption.

Pending Publication Date: 2017-01-18
TONGWEI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the reports on the fermentation of Schizochytrium are all liquid fermentation, which has the problems of complex medium, complex reaction equipment, high energy consumption and high cost.
There is no report on the production of DHA by solid-state fermentation of Schizochytrium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 Fermentation method of the present invention

[0025] 1) Prepare solid-state fermentation substrate.

[0026] The solid-state fermentation substrate selects starchy materials corn flour, bran and rice bran, and the mass ratio of ingredients is 1:1:1; the protein biomass selects soybean meal and rapeseed meal, and the mass ratio of ingredients is 1:1. After the above raw materials are removed from impurities, they are fully mixed according to the mass ratio of starch and protein raw materials of 3:1, sterilized and ready for use.

[0027] 2) Preparation of Schizochytrium seed solution

[0028] The strain used is Schizochytrium sp. SLTW3, which has been preserved in the Type Culture Collection Center in October 2015 with the preservation number CCTCC No: M2015591, which is recorded in the patent application document with the publication number CN105420122A.

[0029] Prepare the Schizochytrium liquid seed culture medium, pick a plate for activation; inoculat...

Embodiment 2

[0036] Embodiment 2 fermentation method of the present invention

[0037] 1) Prepare solid-state fermentation substrate.

[0038] The solid-state fermentation substrate selects starchy materials, corn flour and bran, and the mass ratio of ingredients is 1:1; the protein biomass selects rapeseed meal. The above-mentioned raw materials are fully mixed according to the mass ratio of starch and protein raw materials of 2:1, sterilized and set aside.

[0039] 2) Preparation of Schizochytrium seed solution

[0040] The strain used is Schizochytrium sp. SLTW3, which has been preserved in the Type Culture Collection Center in October 2015 with the preservation number CCTCC No: M2015591, which is recorded in the patent application document with the publication number CN105420122A.

[0041] Prepare the Schizochytrium liquid seed culture medium, pick 1~2 rings of activated Schizochytrium strains from the flat plate and inoculate them into a 250mL triangular flask containing 50mL of the...

Embodiment 3

[0048] Embodiment 3 fermentation method of the present invention

[0049] 1) Prepare solid-state fermentation substrate.

[0050] The solid-state fermentation substrate selects starchy materials, corn flour and bran, and the mass ratio of ingredients is 1:1; the protein biomass selects rapeseed meal. The above-mentioned raw materials are fully mixed according to the mass ratio of starch and protein raw materials of 1.5:1, sterilized and set aside.

[0051] 2) Preparation of Schizochytrium seed solution

[0052] The strain used is Schizochytrium sp. SLTW3, which has been preserved in the Type Culture Collection Center in October 2015 with the preservation number CCTCC No: M2015591, which is recorded in the patent application document with the publication number CN105420122A. Prepare the Schizochytrium liquid seed culture medium, pick 1-2 rings of activated Schizochytrium strains from the plate and inoculate them into a 250mL Erlenmeyer flask containing 50mL of the first-grade...

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Abstract

The invention discloses a method for increasing added values of raw materials of schizochytrium sp. solid-state fermented feed. The method comprises the following steps: (1) fetching a schizochytrium sp. seed solution, uniformly mixing with a nutrient solution according to a ratio of 1:10-1:20 (v / v), and adjusting the pH to 6.0-7.0 to obtain mixed liquid; (2) fetching a solid substrate material, adding into the mixed liquid obtained in following step (1) according to a material-liquid ratio of 1:0.5-1:2 (w / v), uniformly mixing, standing and fermenting for 48-72h at 25-35 DEG C, wherein the solid substrate material is a mixture of starch and a protein. The invention further provides a fermented product prepared with the method and application thereof. With the method, the high-DHA content fermented product can be prepared; the method has simple process steps, low energy consumption, low cost and excellent industrial application prospect.

Description

technical field [0001] The invention relates to the field of fermentation, in particular to a method for increasing the added value of a solid-state fermented feed material by Schizochytrium, a fermentation product prepared by the method and an application thereof. Background technique [0002] Solid state fermentation (Solid State Fermentation, SSF) refers to the use of natural substrates as carbon sources or energy sources, or the use of inert substrates as solid supports, the system is anhydrous or nearly anhydrous fermentation process, and liquid fermentation (Submerged Fermentation ,SmF) compared with solid-state fermentation has the characteristics of simple medium, less investment, low energy consumption, low water content of substrate, simple reaction device, and less waste. [0003] Schizochytrium sp., also known as Schizochytrium sp., belongs to Eumycota, Oomycetes, Saprolegniales, and Thraustochytriaceae. algal marine fungus. The bacterium is single-celled, sphe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/18A23K10/30A23K10/37A23K20/24A23K20/22A23K20/26A23K20/20A23K20/10C12N1/14C12R1/645
CPCC12N1/14Y02P60/87
Inventor 廖利民曾娟苏艳秋石锐张良罗国强
Owner TONGWEI
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