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Microorganism DHA fermentation production technology

A plant and nanotechnology, applied in microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of low bacterial density, low DHA content, difficult industrial production, etc., and achieve stable production process and high purity. , the effect of low cost

Inactive Publication Date: 2013-03-20
于文利
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are generally problems such as low cell density and relatively low DHA content at present, making it difficult to carry out industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0010] In a 1L fermenter, add medium and seed liquid at a concentration of 10%, and carry out two-step fermentation in the fermenter: the first step, the temperature is controlled at 28°C, pH=6, and the fermentation medium is glucose, yeast extract, peptone and inorganic salts, fermented for 2 days; the second step, lowered the temperature to 20°C, and continued to ferment for 3 days. During the fermentation process, the concentration was always controlled at 20% by adding glucose, and the docosahexaenoic acid oil was obtained by fermentation , the DHA content is as high as 55%, and the DHA content is as high as 35%, and the DHA productivity reaches 8.8g / liter. Day.

example 2

[0012] In a 100L fermenter, add medium and seed liquid at a concentration of 10%, and carry out two-step fermentation in the fermenter: the first step, the temperature is controlled at 30°C, pH=6, and the fermentation medium is glucose, yeast extract, peptone and inorganic salts, fermented for 3 days; the second step, lower the temperature to 20°C, and continue to ferment for 3 days. During the fermentation process, the concentration of glucose is always controlled at 20%, and the docosahexaenoic acid oil is obtained by fermentation , the DHA content is as high as 60% oil, the DHA content is as high as 40%, and the DHA productivity reaches 10g / liter. Day.

example 3

[0014] In a 1000L fermenter, add medium and seed liquid at a concentration of 10%, and carry out two-step fermentation in the fermenter: the first step, the temperature is controlled at 30°C, pH=6, and the fermentation medium is glucose, yeast extract, peptone and inorganic salts, fermented for 3 days; the second step, lower the temperature to 20°C, and continue to ferment for 3 days. During the fermentation process, the concentration of glucose is always controlled at 20%, and the docosahexaenoic acid oil is obtained by fermentation , the DHA content is as high as 60%, and the DHA content is as high as 38%, and the DHA production rate reaches 10g / liter. Day.

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Abstract

The present invention discloses a microorganism DHA nutrition oil (docosahexaenoic acid) fermentation production method. According to the method, bacterial Schizochytrium sp. is inoculated into a shake flask to culture, then is inoculated into a seed tank to carry out scale-up culture, and finally is inoculated into a fermentation tank to carry out two-step fermentation, wherein in the first step, a temperature is controlled to 25-30 DEG C, the pH value is 6, a fermentation culture medium comprises glucose, yeast extract paste, peptone and an inorganic salt, and fermentation is performed for 2-3 days, in the second step, cooling is performed to a temperature of 20-25 DEG C, continuous fermentation is performed for 2-3 days, a concentration of the glucose is always controlled to 20% during the fermentation process by additionally supplying the glucose, the amount of microorganisms prepared through fermentation is about 60 g / L, oil content in dry biomass is 50-60%, DHA content is 35-40%, and DHA productivity is 8-10 g / L.day. The microorganism DHA fermentation production technology has characteristics of high DHA yield, high purity, stable production technology, simpleness and low cost.

Description

technical field [0001] The invention relates to a microbial DHA fermentation production technology, which belongs to the technical field of food functional oil production. Background technique [0002] Docosahexaenoic acid (DHA for short) is one of the essential fatty acids for human beings. It cannot be synthesized by itself in the body and must be ingested through food. It can promote the growth and development of brain cells in infants, protect eyesight, treat cardiovascular diseases and improve immunity. A variety of important physiological functions, such as ability, have attracted extensive attention from the medical and food industries. At present, the commercial source of DHA is mainly deep-sea fish oil, but because the DHA content in fish oil is relatively low, there is a risk of heavy metal mercury pollution, and it is increasingly scarce Due to the limitation of marine resources, the use of microbial fermentation to produce DHA is expected to become an effective s...

Claims

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Application Information

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IPC IPC(8): A23D9/04C12P7/64C12R1/645
Inventor 于文利
Owner 于文利
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