Schizochytrium sp. and method for producing DHA lipa by using same
A technology of schizovibrio and oil, which is applied in the field of marine fungus schizovibrio and its high-density fermentation to produce DHA oil, which can solve the problems of low biomass, low production efficiency, and increased production cost, and achieve biomass The effect of high DHA content, reduced fermentation cost, and simple process
Active Publication Date: 2010-12-01
NANJING TECH UNIV
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Among them, there are four papers related to the present invention. The bacteria used in CN200610125476.3 is Cryptodinosaurus, with a biomass of 20-40g / L and an oil content of 20-50% in the cells; CN00135338.1, CN200410075426.X , CN200610028869.2 all adopt Schizovibrio bacterium as the production bacteria, the process and fermentation method are more traditional, only the optimization of simple culture method and culture conditions, there is no detailed study on the key factors that directly affect the biomass accumulation, resulting in the biomass is not High, up to 42.5g / L, did not achieve high-density fermentation in the true sense
Low production efficiency increases production costs, making it difficult for DHA derived from microorganisms to have a significant price advantage over DHA derived from fish oils
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Abstract
The invention discloses a Schizochytrium sp., which has a category name of Schizochytrium sp., is preserved in Common Microorganisms Center of China Committee for Culture Collection of Microorganisms and has a preservation number of CCTCC No.209059. The invention also discloses a method for producing DHA lipa by using the Schizochytrium sp. The method optimizes a strain fermentation medium from the perspectives of osmotic pressure and element supply and combines a fed-batch strategy to achieve high-density fermentation of microalgae, thus the final dry cell weight reaches 70 grams per liter, the lipa content reaches 31.5 grams per liter, and the DHA accounts for more than 35 percent of the total fatty acid content. All indexes of the DHA essential oil obtained by the method accord with thestandard of food additives, and the DHA essential oil contains a bioactive substance of squalene. The method has the advantages of simple entire process, convenient operation, and high biomass and DHA content, reduces the fermentation cost, and is suitable for industrial production.
Description
A kind of schizovibrio and its method for producing DHA oil technical field The invention relates to a marine fungus schizovibrio and a method for producing DHA oil by high-density fermentation thereof, belonging to the field of biotechnology. Background technique Docosahexaenoic acid (DHA) is an important omega-3 long-chain polyunsaturated fatty acid, which has important physiological functions such as promoting brain cell development, lowering blood lipids, protecting eyesight, anti-cancer, and improving immunity. It is widely used In infant food and pharmaceutical industry. Traditional DHA production raw materials mainly come from fish oil, but differences in fish species, seasons, geographical locations and other factors have caused instability in oil content and unsaturated fatty acid content in oil; DHA extracted from fish oil has high cholesterol content and contains a large amount of EPA (Eicosapetaenoic acid, 22:5) and various minerals; coupled with the increasi...
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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/64C12R1/01
Inventor 黄和任路静肖爱华金立晶李娟
Owner NANJING TECH UNIV
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