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Method for co-producing squalene and polyunsaturated fatty acid

A technology of unsaturated fatty acids and squalene, which is applied in the field of co-production of squalene and polyunsaturated fatty acids, can solve the problems of low EPA efficiency and achieve the effect of increasing the content

Active Publication Date: 2022-05-06
CABIO BIOTECH WUHAN CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The source of EPA is mainly fish oil. At present, it is trying to use Schizochytrium to obtain a relatively stable source of EPA. However, in the current application field of Schizochytrium, the efficiency of Schizochytrium to produce EPA is usually low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] In the present invention, the Schizochytrium sp. screened and isolated from seawater is used as the starting strain, and mutagenesis is performed to obtain a Schizochytrium sp. that can simultaneously produce squalene and polyunsaturated fatty acids. .) CABIO-A-2-IV, which has been deposited, and its deposit information is as follows:

[0031] The deposit number is CCTCC NO: M 2021565; the classification name is: Shchizochy trium sp.; the depository unit is: China Center for Type Culture Collection; deposit address: Wuhan University, Wuhan, China, postcode: 430072; the deposit date is May 19, 2021 .

Embodiment 2

[0033] The present embodiment adopts the Schizochytrium sp. (Schizochytrium sp.) CABIO-A-2-IV obtained in Example 1 to ferment, and the specific process is as follows:

[0034] (1) Seed Activation Cultivation: Get the Schizochytrium CABIO-A-2-IV obtained in Example 1 of the present invention and inoculate it into the activation medium for cultivation. The cultivation temperature is 28°C, and the shaking speed of the shaking table is 200r / min. Time 48h, the activation medium is: glucose 4%, sodium glutamate 3%, yeast extract 0.6%, KH 2 PO 4 0.6%, MgSO 4 0.8%, NaCl 2%, CaCl 2 0.03%, pH natural.

[0035] (2) Seed expansion cultivation: inoculate the activated seed culture solution of the shaking cultivation in the above step (1) into the expansion medium according to the inoculation amount of 5% (volume ratio) for cultivation, the cultivation temperature is 28°C, the cultivation time is 48h, and the culture medium is shaken. The shaking speed is 200 rpm, and the expansion m...

Embodiment 3

[0041] The present embodiment adopts the Schizochytrium sp. (Schizochytrium sp.) CABIO-A-2-IV obtained in Example 1 to ferment, and the specific process is as follows:

[0042](1) Seed Activation Cultivation: Get the Schizochytrium mutant strain of the present invention and inoculate it into the activation medium for cultivation, the cultivation temperature is 28°C, the shaking speed of the shaker is 200r / min, and the cultivation time is 48h. For: Dextrose 4%, Monosodium Glutamate 3%, Yeast Extract 0.6%, KH 2 PO 4 0.6%, MgSO 4 0.8%, NaCl 2%, CaCl 2 0.03%, pH natural.

[0043] (2) Seed expansion cultivation: inoculate the activated seed culture solution of the shaking cultivation in the above step (1) into the expansion medium according to the inoculation amount of 5% (volume ratio) for cultivation, the cultivation temperature is 28°C, the cultivation time is 48h, and the culture medium is shaken. The shaking speed is 200 rpm, and the expansion medium in the shake flask ...

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Abstract

The invention relates to the technical field of microorganisms, in particular to a method for co-producing squalene and polyunsaturated fatty acid. According to the invention, schizochytrium limacinum separated from seawater is taken as an original strain, the schizochytrium limacinum capable of producing squalene and polyunsaturated fatty acid at the same time is obtained through mutagenesis, and the content of EPA (eicosapentaenoic acid) and the content of squalene in the product of the schizochytrium limacinum are at a relatively high level. According to the invention, the fermentation process is further optimized, and the pH value in the fermentation process is kept between 7 and 9; the temperature is 28-33 DEG C, the content of squalene is remarkably increased from 16.9% to 30% or above, and the method has important significance in the production field of squalene and polyunsaturated fatty acid.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for co-producing squalene and polyunsaturated fatty acids. Background technique [0002] EPA (Eicosapentaenoic acid): eicosapentaenoic acid, which is a kind of omega-3 polyunsaturated fatty acid, its main function is to lower cholesterol, promote blood circulation, and prevent the formation and development of atherosclerosis, cerebral thrombosis and other cardiovascular diseases. The source of EPA is mainly fish oil. At present, an attempt is made to use Schizochytrium to obtain a relatively stable source of EPA. However, in the current application field of Schizochytrium, the efficiency of Schizochytrium to produce EPA is usually low. Contents of the invention [0003] In order to solve the problems existing in the prior art, the present invention provides a method for co-producing squalene and polyunsaturated fatty acids, and by improving the fermentation pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C12P7/6432C12N1/14C12R1/645
CPCC12P5/007C12P7/6427C12N1/14Y02A50/30
Inventor 余超汪志明陆姝欢赵洒李翔宇吴金勇陈祥松姚建铭
Owner CABIO BIOTECH WUHAN CO LTD
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