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A method for promoting oil production by Schizochytrium

A technology for Schizochytrium and oil, which is applied in the field of promoting oil production by Schizochytrium, and can solve the problems of pollution and restricting the development of ω-3PUFA production.

Active Publication Date: 2020-06-26
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional PUFAs are mainly obtained from fish oil (Marine Drugs, 2013, 11(7): 2259-81), but due to problems such as overfishing and seawater pollution in fisheries, problems such as fish oil production and food safety restrict the production of ω-3PUFA. develop

Method used

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  • A method for promoting oil production by Schizochytrium
  • A method for promoting oil production by Schizochytrium
  • A method for promoting oil production by Schizochytrium

Examples

Experimental program
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Effect test

Embodiment 1

[0030] (1) Insert Schizochytrium ATCC 1381 (derived from ATCC Strain Collection Center, USA) stored in a glycerol tube into a 100mL shaker flask with 20mL seed medium, the inoculum amount is 2% to 10%, at 20 In a shaker at ~30°C, with a rotation speed of 150-200rpm, cultivate for 24-48 hours to obtain first-class seeds;

[0031](2) Insert the primary seeds into a 100mL shake flask with 20mL seed culture medium, the inoculum size is 2% to 10%, and cultivate them in a shaker at 20 to 30°C at a speed of 150 to 200rpm for 24 to 24 hours. 48h, get the secondary seed;

[0032] The pH of the above seed medium is 6-8, which contains carbon source, nitrogen source, phosphorus source, sulfur source, corn steep liquor powder, yeast powder, ammonium sulfate and sodium glutamate, the carbon source is glucose, and the nitrogen source includes organic nitrogen Source and inorganic nitrogen source, phosphorus source is potassium dihydrogen phosphate or dipotassium hydrogen phosphate, sulfur ...

Embodiment 2

[0044] The para-derivative of benzoic acid to be added is p-toluic acid, and the addition method is as follows: dissolve p-toluic acid in absolute ethanol to make a 100×~1000× concentrated solution, filter and sterilize through a 0.22 μm PVDF filter membrane Finally, take an appropriate amount and add it to the fermentation medium so that the final concentration is 0-3g / L. Take the fermentation broth from the 4th to 7th day to measure the total oil content under different p-toluic acid concentrations, and analyze and identify the fatty acids. All the other are with embodiment 1, concrete result is as figure 2 shown.

Embodiment 3

[0046] The para derivative of benzoic acid to be added is folic acid, and the addition method is as follows: dissolve folic acid in absolute ethanol to make a 100×~1000× concentrated solution, filter and sterilize through a 0.22 μm PVDF filter membrane, then take an appropriate amount and add it to the fermentation medium , so that the final concentration is 100mg / L~3g / L, respectively get the fermented liquid of the 4th~7d to measure the content of total oil under different folic acid concentrations, and carry out analysis and identification to fatty acid, all the other are the same as embodiment 1, concrete result is as follows image 3 shown.

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Abstract

The invention discloses a method for promoting schizochytrium to produce oil. A benzoic acid para-derivative with the final concentration being 100 mg / L-3g / L is added to a schizochytrium fermentation culture medium once at any time from starting of fermentation culture to 1-2 d before ending of fermentation culture. The oil yield is increased by 32.12% by adding a proper amount of sodium benzoate in the benzoic acid para-derivative to the fermentation culture medium, the oil yield is increased by 38.21% by adding p-toluic acid, the oil yield is increased by 56.84% by adding folic acid, the oil yield is increased by 66.51% by the aid of p-aminobenzoic acid, but the added substances have no inhibition effect on omega-3PUFA.

Description

technical field [0001] The invention belongs to the technical field of materials for sports shoes, and in particular relates to a method for promoting oil production by Schizochytrium. Background technique [0002] Polyunsaturated fatty acids (polyunsaturated fatty acids, PUFAs) have important functions in the human body, such as: maintaining the structure and function of cell membranes, reducing cholesterol and triglycerides and other functions. PUFAs are divided into two series, omega-6 (or omega-6) and omega-3 (or omega-3). Most people can get enough omega-6 PUFA from diet, but may lack the right amount of omega-3 PUFA. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the omega-3PUFA series have attracted widespread attention because of their important effects on the human body (Comprehensive Reviews in Food Science & Food Safety, 2010, 9(6): 655-675; International Food Research Journal, 2011, 18: 493-499; Marine Drugs, 2013, 11(7): 2259-81). EPA has a good...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/645
CPCC12P7/6427
Inventor 何宁孟彤李志朋
Owner XIAMEN UNIV
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