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Bacterium strain for producing docosahexaenoic acid and use thereof

A technology of docosahexaenoic acid and microbial strains, which is applied in the fields of industrial microorganisms, food and feed industries, and can solve problems such as cost reduction, limited technical indicators such as growth rate, and inability to further increase production.

Active Publication Date: 2017-07-28
XIAMEN HUISON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The marine microorganisms used to ferment and produce DHA are mainly Schizochytrium (Schizochytrium). Increase production and reduce costs

Method used

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  • Bacterium strain for producing docosahexaenoic acid and use thereof
  • Bacterium strain for producing docosahexaenoic acid and use thereof
  • Bacterium strain for producing docosahexaenoic acid and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. Isolation, Identification and Preservation of Schizoochytrium limacinum HS01CGMCC No.13746

[0046] 1. Isolation of Schizochytrium HS01

[0047] 1. The inventor of the present application collected Schizochytrium fungus from many places in the mangrove forest of Yunxiao County, Zhangzhou City, Fujian Province, and mixed them to obtain a mixed solution; inoculated 0.5mL of the mixed solution into 5mL of the screening liquid medium, and then 25°C, 200rpm / min Cultivate for 2 days to obtain culture broth.

[0048] 2. Spread the cultured bacteria solution obtained in step 1 evenly on the screening plate, and culture it at 25°C for 2 days to produce a single colony.

[0049] 3. After completing step 2, pick a single colony and inoculate it into 5 mL of fermentation medium, and then culture at 25°C and 200 rpm / min for 2 days to obtain a culture liquid.

[0050] 4. Take the cultured bacteria liquid obtained in step 3, centrifuge at 4°C and 2000 rpm for 5 minutes, a...

Embodiment 2

[0065] Embodiment 2, Schizoochytrium limacinum (Schizoochytrium limacinum) HS01 fermentation production of DHA

[0066] 1. Fermentation of Schizoochytrium limacinum HS01 to produce DHA

[0067] 1. Inoculate a single clone of Schizoochytrium limacinum HS01 into a shaker flask (10mL) containing 2mL shaker medium, and culture it at 22-28°C and 150-250rpm / min for 24-48h to obtain a grade seed liquid.

[0068] 2. Take the first-grade seed liquid, inoculate it into a shaker flask (the shaker flask specification is 1L) with 250mL of shaker flask culture medium at an inoculation amount of 3-10% (v / v), at 22-28°C, 150-250rpm / min and cultured for 24-48 hours to obtain secondary seed liquid.

[0069] 3. Take the secondary seed liquid, inoculate it into a fermenter (the specification of the fermenter is 5L) with 3L seed culture medium with an inoculation amount of 3-10% (v / v), and cultivate it at 22-28°C for 24-48h ( The dissolved oxygen is 10-80%) to obtain the first-grade fermented se...

Embodiment 3

[0083] Embodiment 3, Schizoochytrium limacinum (Schizoochytrium limacinum) HS01 large-scale fermentation production of DHA

[0084] 1. Inoculate a single colony of Schizoochytrium limacinum HS01 into a shaker flask (250mL shaker flask size) filled with 20mL shaker flask medium, and culture at 22-28°C, 150-250rpm / min for 24-48h, Obtain the primary seed solution.

[0085] 2. Take the primary seed solution and inoculate it into a shaker flask (2L shaker flask specification) with 3-10% (v / v) inoculum amount, at 22-28°C, 150-250rpm / min and cultured for 24-48 hours to obtain secondary seed liquid.

[0086] 3. Take the secondary seed liquid, inoculate it into a fermenter (the specification of the fermenter is 1000L) equipped with 500L seed culture medium with an inoculation amount of 3~10% (v / v), and cultivate it at 22~28°C for 24~48h ( Dissolved oxygen is 10-80%) to obtain the first-stage fermented seed liquid with a biomass of 15-30 g / L.

[0087] 4. Take the fermented primary s...

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Abstract

The invention discloses a bacterium strain for producing docosahexaenoic acid and a use thereof. The bacterium strain for producing docosahexaenoic acid is Schizoochytrium limacinum HS01, is preserved in the China general microbiological culture collection center and has the accession number of CGMCC No. 13746. The experiment results show that Schizoochytrium limacinum HS01 is subjected to fermentation culture so that fermentation broth is obtained, and the fermentation broth has DHA content of 45.0% to 60.0% in oil and thus the bacterium strain has an important application value.

Description

technical field [0001] The invention relates to the fields of industrial microbes, food and feed industries, in particular to a docosahexaenoic acid-producing bacterium and its application. Background technique [0002] Docosahexaenoic acid (DHA) is an essential polyunsaturated fatty acid for the human body. In terms of molecular structure, DHA is a straight-chain fatty acid containing 22 carbon atoms and 6 double bonds. Because the first double bond appears on the third carbon atom at the methyl end of the fatty acid chain, it belongs to the omega-3 series of fatty acids (OMEGA-3). DHA has important physiological functions, such as promoting nervous system development, improving retinal function, improving vision, preventing cardiovascular diseases, treating cardiovascular diseases, anti-inflammation and inhibiting allergic reactions, etc. Since the DHA in the daily diet is often insufficient, adding DHA to food or milk powder is of great significance to humans, especiall...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/64C12R1/645
CPCC12N1/14C12P7/6427C12N1/145C12R2001/645
Inventor 陈礼毅钟惠昌陈水荣
Owner XIAMEN HUISON BIOTECH
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