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Preparation method of algal oil DHA

A technology of algal oil and microalgae, applied in the field of health food, can solve the problems of low yield, backward preparation technology, low production efficiency, etc., and achieve the effects of considerable economic value and high product purity

Inactive Publication Date: 2018-09-04
富诺健康股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the existing preparation technology for extracting DHA from microalgae is relatively backward, with low production efficiency and low yield rate, and cannot efficiently realize large-scale factory production and preparation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (1) select Schizochytrium to cultivate and ferment; said culturing and fermenting are algae culturing and heterotrophic fermentation to obtain microalgae raw materials;

[0050](2) drying; the Schizochytrium raw material obtained in step (1) is dried to obtain the Schizochytrium powder;

[0051] (31) A certain amount of Schizochytrium powder is placed in a beaker, a small amount of extraction solvent is added, put into an ultrasonic water bath, and ultrasonic pretreatment is carried out for a certain period of time under a certain ultrasonic power;

[0052] (32) After ultrasonic pretreatment, the microalgae in the beaker are suction-filtered;

[0053] (33) Schizochytrium powder is packed into filter paper tube, and filtrate is poured into round-bottomed flask, and as the extraction solvent of Soxhlet extraction, Soxhlet extractor is installed;

[0054] (4) Extraction of algae oil: take Schizochytrium algae powder, use 60% ethanol as the extraction agent by mass fractio...

Embodiment 2

[0063] (1) Selecting Cryptidium coirii to cultivate and ferment; said cultivation and fermentation are algae cultivation and heterotrophic fermentation, so as to obtain the Cryptidium coirii raw material;

[0064] (2) Drying; the Cryptidium kelp raw material obtained in step (1) is dried to obtain the Cryptidium kelp powder;

[0065] (31) A certain amount of Cryptidinium coirii powder is placed in a beaker, a small amount of extraction solvent is added, put into an ultrasonic water bath, and ultrasonic pretreatment is carried out for a certain period of time under a certain ultrasonic power;

[0066] (32) After ultrasonic pretreatment, the microalgae in the beaker are suction-filtered;

[0067] (33) Cryptidium koii powder is packed into filter paper tube, and filtrate is poured into round-bottomed flask, and as the extraction solvent of Soxhlet extraction, Soxhlet extractor is installed;

[0068] (4) Algae oil extraction: take Cryptidinium kelp algae powder, use 70% ethanol a...

Embodiment 3

[0077] (1) select Isochrysis to cultivate and ferment; described cultivation and fermentation are algae cultivation and heterotrophic fermentation, obtain Isochrysis raw material;

[0078] (2) drying; the Isochrysis raw material obtained in step (1) is dried to obtain Isochrysis powder;

[0079] (31) Place a certain amount of Isochrysis powder in a beaker, add a small amount of extraction solvent, put it into an ultrasonic water bath, and carry out ultrasonic pretreatment for a certain period of time under a certain ultrasonic power;

[0080] (32) After ultrasonic pretreatment, the microalgae in the beaker are suction-filtered;

[0081] (33) Isochrysis powder is packed into filter paper tube, and filtrate is poured into round-bottomed flask, and as the extraction solvent of Soxhlet extraction, Soxhlet extractor is installed;

[0082] (4) Extraction of algae oil: take Isochrysis algae powder, use 800% ethanol as the extraction agent in mass fraction, and use an accelerated sol...

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Abstract

The invention discloses a preparation method of algal oil DHA. The preparation method comprises following steps: 1, selected microalgae is subjected to algae seed culturing and heterotrophic fermenting so as to obtain a microalgae raw material; 2, the microalgae raw material is subjected to drying treatment so as to obtain microalgae powder; 3, the microalgae powder is subjected to microalgae wallbreaking pre-treatment so as to obtain dried microalgae powder; 4, algal oil is extracted, wherein the dried microalgae powder is taken as a raw material, an ethanol solution with a mass concentration of 60 to 100% is taken as an extractant, an accelerated solvent extraction instrument is adopted for extraction, an obtained extract is introduced into a vacuum rotatory evaporator for organic solvent evaporation, and a residue obtained through evaporation is the algal oil; 5, urea coating enrichment purifying is carried out; and 6, filtering condensation is carried out so as to obtain algal oilDHA finished products. The product purity of the preparation method is high, defects in preparation of DHA from fish oil and microalgae are avoided, and the finished product can be taken as a raw material in the fields of food, health care product, food additive, and high grade chemical product.

Description

technical field [0001] The invention relates to the technical field of health food, in particular to a preparation method of algae oil DHA. Background technique [0002] The important physiological functions of DHA in the central nervous system, cardiovascular system and visual nervous system have been widely recognized by society, and many countries in the world have stipulated that DHA can be used as a nutritional fortifier and food additive. At present, DHA has been used in infant food, dairy products, beverages, soybean milk and edible oils, etc., and the market demand is very large. Therefore, the production of DHA contains huge business opportunities. [0003] The main sources of DHA are fish oil and algae oil. Fish oil has a large fishy smell and a high content of EPA, and there are certain difficulties in food addition, and the number of people who eat it is also limited, and fish oil resources are limited. However, large-scale, high-density microalgae heterotrophi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/6427
Inventor 程彦邓颖妍
Owner 富诺健康股份有限公司
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