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Dehydroacetic acid (DHA) feed additive prepared from kitchen waste and preparation method thereof

A docosahexaenoic acid and kitchen waste technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of reducing the production cost of the medium and the effect is not significant, and achieve product additional High value, no need for organic reagent extraction, high DHA content

Active Publication Date: 2015-02-11
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The soybean meal hydrolyzate and straw enzymolyzate used in patents CN101824442B and CN103614427A can only partially replace the nitrogen source or carbon source in the fermentation medium, and the effect of reducing the production cost of the medium is not significant

Method used

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  • Dehydroacetic acid (DHA) feed additive prepared from kitchen waste and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] (1) Pretreatment: collect fresh and undeteriorated food waste from the canteen of a scientific research unit in the south, and manually assist machines to remove inorganic or refractory debris such as glass, ceramics, plastics, metals, bones, and toothpicks, and obtain the after-cleaning waste. kitchen waste; the food waste is pulverized with a food tissue grinder, diluted with water at a volume ratio of 1:1, and homogenized with a homogenizer to obtain a homogenate, so that the particle size of the material in the homogenate is within Below 5mm;

[0069] The moisture content of the food waste used this time is 72.1%, TS is 13.8%, VS / TS is 86.4%, salt content is 1.51%, and the chemical composition of the food waste is 23.4% total sugar and 27.7% protein on a dry basis , crude fat 19.4%, crude fiber 7.5%, ash 9.3%.

[0070] (2) Segmented enzymatic hydrolysis: heat the homogenate in step (1) to 80°C, add α-amylase 15U / (g kitchen waste), and enzymatically hydrolyze for 10...

Embodiment 2

[0079] (1) Pretreatment: collect fresh and undeteriorated food waste from the canteen of a scientific research unit in the south, and manually assist machines to remove inorganic or refractory debris such as glass, ceramics, plastics, metals, bones, and toothpicks, and obtain the after-cleaning waste. kitchen waste; use a food tissue grinder to pulverize the food waste, dilute it with water at a volume ratio of 1:2, and use a homogenizer to obtain a homogenate, so that the particle size of the material in the homogenate is within Below 10mm;

[0080] The moisture content of the food waste used this time is 63.3%, TS is 17.4%, VS / TS is 92.6%, salt content is 1.21%, and the chemical composition of the food waste is 44.3% of total sugar and 20.1% of protein on a dry basis. , crude fat 9.7%, crude fiber 5.9%, ash 8.5%.

[0081] (2) Segmented enzymatic hydrolysis: heat the homogenate to 70°C, add α-amylase 8U / (g kitchen waste), and enzymolyze for 80 minutes to obtain the first enz...

Embodiment 3

[0090] (1) Pretreatment: collect fresh and undeteriorated food waste from the canteen of a scientific research unit in the south, and manually assist machines to remove inorganic or refractory debris such as glass, ceramics, plastics, metals, bones, and toothpicks, and obtain the after-cleaning waste. kitchen waste; use a food tissue grinder to pulverize the food waste, dilute it with water at a volume ratio of 1:1, and use a homogenizer to obtain a homogenate, so that the particle size of the material in the homogenate is within Below 10mm;

[0091] The moisture content of the food waste used this time is 63.3%, TS is 17.4%, VS / TS is 92.6%, salt content is 1.21%, and the chemical composition of the food waste is 44.3% of total sugar and 20.1% of protein on a dry basis. , crude fat 9.7%, crude fiber 5.9%, ash 8.5%.

[0092] (2) Segmented enzymatic hydrolysis: heat the homogenate to 90°C, add α-amylase 18U / (g kitchen waste), and perform enzymatic hydrolysis for 60 minutes to o...

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Abstract

The invention discloses a dehydroacetic acid (DHA) feed additive prepared by fermenting kitchen waste and a preparation method thereof. The DHA feed additive is prepared by carrying out spray drying on a fermentation solution of a DHA-producing strain; and the DHA content in the DHA feed additive is higher. The preparation method comprises the following steps: carrying out pretreatment and sectional enzymolysis on the kitchen waste to obtain an enzymolysis composite, and centrifugating the enzymolysis composite to obtain a precipitate which can be made into feed albumen powder and an enzymolysis supernatant which can be made into a fermentation culture medium; autoclaving, and inoculating the DHA-producing strain to perform fermentation, thereby obtaining the fermentation solution; and adding an antioxidant and / or a flocculant into the fermentation solution to obtain the DHA feed additive. The kitchen waste enzymolysis solution can be simultaneously substituted for the carbon source and nitrogen source in the culture medium, thereby lowering the production cost of the culture medium, further greatly lowering the production cost of the DHA feed additive, and implementing the maximized utilization of the kitchen waste resources.

Description

technical field [0001] The invention relates to the field of feed processing and the technical field of resource utilization of kitchen waste, in particular to a feed docosahexaenoic acid additive prepared from kitchen waste and a preparation method thereof. Background technique [0002] Docosahexaenoic acid (DHA) is an important unsaturated fatty acid, which can be used as a nutritional ingredient mixed with feed antioxidants as a feed additive. The addition of DHA in feed plays an important role in the growth and development of animals, improving health and preventing diseases. [0003] The current commercial source of DHA is mainly deep sea fish oil. However, the DHA content in fish oil is low, and the increasingly scarce marine resources are difficult to meet the market demand for DHA. In recent years, the use of microbial fermentation to produce DHA has received widespread attention. The medium is the basis of fermentation, which has an important impact on the growth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64A23K1/16C12R1/645
CPCC12P7/6427
Inventor 梁岩刘春花徐芳芳
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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