70 results about "Ergostanol" patented technology

The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.

The invention discloses a cluster naematolin ligarine extract, which separates ergosterin peroxidate and two monomer compounds of ergosterin. The invention also provides making method of extract from cluster naematolin fruiting body and mycelium for industrial manufacturing, which is fit for treat tumour drug.

The invention relates to a preparation method of a plant growth regulator, in particular to a preparation method of 24-epibrassinolide, which belongs to the chemical field, and comprises the following steps that: 1. starting from ergosterol 1, an intermediate body 3 is prepared through two reactions, i.e. methyl sulfonyl and hydrolysis according to a traditional synthesis process; 2. the intermediate body 3 is oxidized by a novel oxidation system osmium tetroxide / inter-chloro benzoic acid peroxide to prepare an intermediate body 4; and 3. the intermediate body 4 is finally converted into the 24-epibrassinolide 8 through four reactions, i.e. reduction, rearrangement, dihydroxylation and lactonization according to the traditional synthesis process. Compared with the traditional oxidation system chromium trioxide / pyridine used in the oxidization of the intermediate body 3 into the intermediate body 4, the novel oxidation system used by the invention has the advantages of convenient operation and little pollution, and is more applicable to industrial production.

The invention provides an ergosterin derivative which is obtained by extraction, separation and purification from traditional Chinese medicine ganoderma lucidum spore powder. In the invention, an anti-aging yeast model, namely a K6001 yeast cell and a mutant strain thereof (delta uth1 and delta skn7) prove that the ergosterin derivative influences the gene expression of the UTH1 by regulating the activity of the Skn7 so as to prolong the duplicability life of yeast. The compound is used as a lead compound to optimize the structure, and can be used for preparing medicaments for delaying aging and preventing or / and treating aging diseases. The general structure formula of the ergosterin derivative is as shown in the specification.

The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.

The invention belongs to the technical field of organic synthesis. Based on a seven-step synthesis process, p-toluene sulfonyl chloride is used as an acylation reagent; a manganese dioxide / air catalytic system is used in a water and methanol system; and a calcium / HMPA / R1(CH2)nR2 reduction system is used. According to the synthesis method of the 24-epibrassinolide, ergosterol is used as a startingraw material, a seven-step synthesis process is adopted, the method comprises esterification, hydrolysis, oxidation, reduction, rearrangement, dihydroxylation and lactonization; methylbenzene is usedas a solvent in the esterification process, and ergosterol reacts with benzene sulfonyl chloride in the presence of an acid-binding agent; wherein a manganese dioxide / air system is adopted in the oxidation process, methanol is used as a solvent, and oxidation is performed in air; and a calcium / R1(CH2)nR2 / / HMPA / reduction system is adopted in the reduction process. According to the synthesis method,the total yield is increased by 20 points or above, the product quantitative content is 85% or above, the reaction condition is mild, the technological process is short, the operation is convenient,manganese dioxide can be recycled, low toxicity and low environmental pollution are achieved, and the synthesis method is suitable for industrial production of 24-epibrassinolide.

The invention relates to a preparation method to produce ergosterol with yeast in fine chemical industry, which comprises: mixing the yeast, base and sodium chloride solution for saponification, extracting by toluene, cleaning with water, condensing, refining, and drying in vacuum. This method has convenient process with low cost and provides material guarantee for vitamin D2.

The invention provides a compound antibacterial agent and a preparation method thereof. The compound antibacterial agent contains the following components in parts by weight: 111 parts of water, 0.5-11 parts of soybean extractive solution, 0.5-2.5 parts of glycine, 0.005-3.0 parts of epsilon-poly-lysine, 0.1-5.0 parts of benzalkonium chloride, 0.01-0.1 part of ergosterol, 0.1-1 part of docosanoic acid, and 0.1-6.0 parts of didecyldimethylammonium chloride. The preparation method of the compound antibacterial agent comprises the steps of diluting soybean extractive solution with water, and proportionally adding glycine, epsilon-poly-lysine, benzalkonium chloride and didecyldimethylammonium chloride. The compound antibacterial agent provided by the invention is not harmful to human and livestock, has high sterilizing capacity, can be used at various sterilizing and disinfecting sites, and has the advantages of low cost, wide application range and good sterilizing effect.

The invention discloses a preparation method of a 24-epibrassinolide intermediate, and belongs to the field of chemical synthesis, and the preparation method comprises the following steps: 1) taking ergosterol as an initial raw material, carrying out methanesulfonylation under the catalysis of tetrabutylammonium hydrogen sulfate to obtain a first-step product, namely an intermediate 1, filtering afirst-step reaction solution, and directly carrying out a next-step reaction on a filtrate; 2) adding water and potassium bicarbonate into the filtrate obtained in the step 1), carrying out heating reaction, and carrying out split-phase water removal, concentration, cooling and filtration to obtain an intermediate 2; 3) reacting the intermediate 2 with benzyl halide in the presence of an acid-binding agent to generate an etherification product; 4) reacting the etherification product with tetrabutylammonium bromide and tetrabutylammonium hydrogen persulfate to obtain an intermediate 3; compared with a traditional CrO3 / pyridine oxidation method, the method has the advantages that the use of an oxidizing agent containing heavy metal chromium and having serious pollution to the environment isavoided, the reaction conditions are mild, the yield is high, the post-treatment is simple, the next step of reaction can be performed without refining, and the method is suitable for industrial production.

The invention relates to a construction method and application of saccharomyces cerevisiae capable of dynamically regulating and controlling 7-deoxycholesterol and vitamin D3. The construction method comprises the following steps: S1, controlling expression of a saccharomyces cerevisiae engineering strain mot3 gene by using a promoter GAL7, and transforming the mot3 gene into original saccharomyces cerevisiae to construct first saccharomyces cerevisiae; S2, constructing a dCas9 system dynamically regulated and controlled by ergosterol, and extracting constructed plasmids; S3, artificially synthesizing a fusion gene segment gal80F-loxT-PTEF1-DHCR24-Tcyc1-PGAP-DIC-TADH1-gal80R, convertingthe fusion gene segment into a first saccharomyces cerevisiae, and carrying out PCR verification, so as to obtain a second saccharomyces cerevisiae; and S4, transforming the plasmids constructed in the step S3 into second saccharomyces cerevisiae, and performing screening to obtain the saccharomyces cerevisiae capable of dynamically regulating and controlling to produce 7-DHC. According to the saccharomyces cerevisiae constructed by the construction method provided by the invention, the reduction of by-products and the increase of the yield of 7-DHC are realized by utilizing an endogenous sterol regulation and control system of the saccharomyces cerevisiae and combining a dCas9 system while the growth state of a strain is not influenced.

The invention belongs to the field of composition detection of traditional Chinese medicinal materials, and particularly relates to a method for simultaneously detecting uridine and ergosterol and application thereof. The method comprises the following steps that a test solution is prepared; a mixed reference solution is prepared; and HPLC measurement is carried out, and specifically High Performance liquid Chromatography detection is carried out on the test solution and the mixed reference solution, wherein the sample feeding amount of the test solution is 0.5-2.5 microliters. The method overcome the defects of peak collapse, asymmetric peak shape and the like which occur when the uridine is extracted and analyzed by using an organic solvent, simultaneous detection of the uridine and theergosterol is realized, and meanwhile, the detection method is stable, reliable and wide in application range.

The invention discloses an all-component extraction method of lucid ganoderma. The method comprises the following steps: carrying out low-temperature ultramicro molecular grinding, carrying out supercritical carbon dioxide extraction on lucid ganoderma essence powder, extracting triterpenoids with alcohol, extracting ganoderan, extracting protein, extracting other active substances, extracting nutrient substances such as amino acids and the like, and extracting residual nutrient components. Most nutritional ingredients and active ingredients in the lucid ganoderma can be obtained through classified extraction, different ingredients are used for different purposes, the overall utilization rate of the lucid ganoderma is far higher than that of a common extraction method, the total yield of the extract can reach 65% or above of the dry weight of the lucid ganoderma, and the purity of the extract is higher; in the preparation process, components such as ganoderma triterpenes, ganoderan, proteins, adenosine, mannitol, ergosterol, vitamins and amino acids are obtained through low-temperature wall breaking, fractional extraction and respective purification, and the preparation method hasthe characteristics of being simple, high in extraction rate and high in total product specific gravity.

The invention provides a saccharomyces cerevisiae engineering bacterium for producing rapeseed sterol and a construction method and application thereof, which belong to the technical field of metabolic engineering. The saccharomyces cerevisiae engineering bacterium contains sterol delta 7-reductase genes and glucose dehydrogenase genes. The saccharomyces cerevisiae engineering bacterium provided by the invention can be used for reducing ergosterol into rapeseed sterol. According to the invention, sterol delta 7-reductase and glucose dehydrogenase are co-expressed in the saccharomyces cerevisiae engineering bacterium; and NADPH formed by the glucose dehydrogenase can be used as a cofactor, and the sterol delta 7-reductase is used for reducing inherent ergosterol of saccharomyces cerevisiaeinto rapeseed sterol, so that the purpose of biologically preparing rapeseed sterol is achieved.

The invention relates to the field of health-care medicinal preparations, and particularly discloses a preparation method of sporoderm-broken ganoderma lucidum spore powder capsules and the sporoderm-broken ganoderma lucidum spore powder capsules. The preparation method of the sporoderm-broken ganoderma lucidum spore powder capsules comprises the following steps: uniformly mixing 94-98% of sporoderm-broken ganoderma lucidum spore powder and 2-6% of ganoderma lucidum fine powder in percentage by mass, and performing granulating and freeze-drying to obtain freeze-dried granules of which the water ratio is less than 7%; and filling capsules to obtain the sporoderm-broken ganoderma lucidum spore powder capsules. The sporoderm-broken ganoderma lucidum spore powder is prepared through the procedures of cleaning, soaking, filtering, freeze drying and sporoderm breaking; and the ganoderma lucidum fine powder is prepared through the procedures of soaking, washing, extracting, concentrating, alcohol precipitating and drying. The contents of polysaccharides, spore oil and ergosterol in the sporoderm-broken ganoderma lucidum spore powder capsules prepared by the method are high, and meanwhile,the active ingredients have the advantage of being easily absorbed and utilized by a human body. The invention also correspondingly discloses the sporoderm-broken ganoderma lucidum spore powder capsules which are prepared by the preparation method.

The invention relates to a preparation method of RGD cyclic peptide R8 peptide modified ergosterol and gefitinib combined compound liposome freeze-dried powder. The preparation method comprises the following steps: adding a freeze-drying protective agent into a pre-prepared RGD / R8-ERG / GEF-LIP liposome suspension in an external addition manner; and finally, preparing the freeze-dried powder of thecompound liposome by adopting a freeze-drying method. The RGD / R8-ERG / GEF-LIP lipidosome suspension is prepared by adopting the following method: firstly, preparing ERG / GEF-LIP, and then, preparing theRGD / R8-ERG / GEF-LIP lipidosome suspension by adopting a post-insertion method. According to the invention, an RGD / R8-ERG / GEF-LIP active drug-loading liposome drug delivery system is successfully constructed; ERG / GEF-LIP is used for investigating a freeze-drying process and a prescription, and after an optimal prescription process is screened out, the optimal prescription process is applied to RGD / R8-ERG / GEF-LIP for verification. An in-vitro test result of RGD / R8-ERG / GEF-LIP freeze-dried powder proves that the RGD / R8-ERG / GEF-LIP freeze-dried powder has a relatively strong tumor cell proliferation inhibition effect, the fluorescence uptake intensity and the good cell apoptosis rate.

The invention discloses a preparation and extraction process of ganoderma lucidum spore powder zymolyte, a product of the ganoderma lucidum spore powder zymolyte and application of the ganoderma lucidum spore powder zymolyte in preparation of a compound preparation with a sleep improving effect. The preparation and extraction process comprises the following steps: taking ganoderma lucidum spore powder as a raw material, carrying out protease enzymolysis to obtain a ganoderma lucidum spore powder zymolyte crude product; and filtering, concentrating and drying to obtain the ganoderma lucidum spore powder zymolyte. The prepared ganoderma lucidum spore powder zymolyte is rich in water-soluble functional substances such as crude polysaccharides, total triterpenes, small peptides and amino acids, and alcohol-soluble active components such as ganoderic acid, saponin and ergosterol, and then the ganoderma lucidum spore powder zymolyte, spina date seeds and honey powder are used as active components to form the compound preparation, so that the compound preparation has an excellent sleep improving effect, the sleep latency period can be shortened, the sleep proportion can be increased, and the sleep time can be prolonged.

The invention discloses a device for saponifying and extracting ergosterol, which comprises an extraction tank. The extraction tank comprises an upper cylindrical barrel and a lower conical barrel which are connected up and down, a feeding port is formed in the top of the extraction tank, a discharging port is formed in the bottom of the extraction tank, and a stirring device is arranged in the center of the extraction tank; and a flow baffle is arranged on the inner wall of the extraction tank. The method has the advantages of simplified extraction process, short extraction time, low energy consumption in the extraction process, high product purity and the like.

The invention discloses a method for increasing the content of adenosine and ergosterol in a fungus mixed fermentation broth, and relates to the technical field of edible fungus mixed fermentation, in particular to a method for increasing the content of adenosine and ergosterol in a fungus mixed fermentation broth, which comprises the following steps: (1) firstly, respectively preparing a plurality of medicinal and edible fungus slant mother strains at the culture temperature of 20-25 DEG C and with the humidity of 45-65% and the culture time of 7-10 days; and (2) after the mother strains are mature, respectively inoculating seed fermentation shake flasks, placing 5-8 glass beads in each flask, and controlling the inoculation amount to be 5-8 square centimeters, wherein the culture temperature is 20-25 DEG C, the rotating speed is 180-240 rpm, and the culture time is 56-72 hours. According to the invention, when mixed culture of medicinal and edible fungi is studied, it is found that the content of adenosine and sterol can be remarkably increased by combining static culture and dynamic culture; and then, the method for increasing the content of adenosine and sterol is further optimized in the invention.

The invention discloses a saccharomyces cerevisiae engineering strain capable of efficiently producing ergosterol, the saccharomyces cerevisiae engineering strain is constructed by overexpressing a lipid transporter gene SEC14 as shown in SEQ ID NO.1 in a saccharomyces cerevisiae host, the nucleotide sequence of the lipid transporter gene SEC14 is SEQ ID NO.1, and the amino acid sequence coded by the lipid transporter gene SEC14 is SEQ ID NO.2. The invention also discloses a saccharomyces cerevisiae engineering strain capable of efficiently producing ergosterol. The ergosterol contained in the constructed saccharomyces cerevisiae engineering strain is 0.95%-1.20% of the dry weight of saccharomycetes, and is 26%-31% higher than that of a wild type saccharomycetes strain. The engineering bacterium provided by the invention not only has the advantage of high yield, but also can be fermented through relatively simple method conditions, is easy to implement and control in industry, is low in investment cost, and has a wide application prospect.

The invention discloses a method for preparing an armillaria luteo-virens fruiting body anti-hepatoma activity sterol compound. The method is simple in technology, high in recovery rate, good in repeatability, and stable and controllable in quality. Moreover, a cell growth curve is used as the basic index for judging the influence of standard components and samples on the cell physiological activity such as adhesion to the wall and reproduction. The results are visual and easy to judge. The obtained armillaria luteo-virens fruiting body anti-hepatoma activity constituent is judged to contain ergosterol and 5 alpha-ergosta-7,22-dien-3 beta-ol through further separation, crystallization treatment and NMR carbon spectrum and hydrogen spectrum analysis.

The invention discloses an anti-static polybutylene-based packaging film material and a preparation method thereof. The packaging film material is prepared from, by weight, polybutylene, poly(butylene succinate), polycaprolactone, polyamide fibers, modified carbon nanotubes, indium oxide, itaconic acid, dicumyl peroxide, an ethylene-vinyl acetate copolymer, ergosterol, sodium fluoride, ethylene bis stearamide, vinyl acetate, tetramethyl thiuram disulfide, ethoxylation alkylamine and 2-hydroxy-4-methoxybenzophenone. The packaging film material prepared through the method is good in anti-static property, good in barrier property to water vapor and oxygen, excellent in mechanical property and capable of meeting the usage requirements as a packaging membrane material.

The present disclosure relates to a drug combination for treating non-small cell lung cancer, in particular to uses of ergosterol combined with gefitinib, and belongs to the technical field of biomedicine. In the present disclosure, the mechanism of action that ergosterol (ERG) combined with gefitinib (GEF) induces apoptosis of non-small cell lung cancer (NSCLC) cells is firstly studied; then an RGG cyclic peptide / R8 peptide modified ERG combined with GEF active drug-loaded targeted liposome delivery system (RGD / R8-ERG / GEF-LIP) is constructed; and the RGD / R8-ERG / GEF-LIP is prepared into a freeze-dried powder to improve stability, quality evaluation and preliminary evaluation of in vitro anti-lung cancer effects are conducted, and a nude mouse lung cancer xenograft model is established for conducting preliminary pharmacodynamic research and in vivo targeting research.

The invention discloses an improved washing agent composition. The improved washing agent composition is prepared from, by weight, 5.2-8.7 parts of ammonium molybdate, 2.5-5.3 parts of ergosterol, 2.7-7.3 parts of isomeric heptanes, 5.2-8.3 parts of a dispersing agent, 3.8-5.3 parts of carboxymethyl fiber starch, 5.1-6.3 parts of aminobenzene sulfonamide, 3.2-4.5 parts of 2-methyl propane sulfonic acid and 10-12 parts of water. The improved washing agent composition has strong infiltration capacity and can infiltrate to the bottom layer of a cleaned object and quickly dissolve and remove various dirt and impurities attached to the surface of a metal part, a redeposition phenomenon in cleaning is avoided, and corrosion and damage to the metal surface in the cleaning process are avoided; the cleaning speed is high, the cleaned metal surface is clean and bright, the metal surface quality is good, and metal machining precision can be effectively guaranteed.

The invention relates to a preparation method for producing ergosterol by using yeast, belonging to the technical field of fine chemical engineering. The preparation method comprises the following steps of: mixing the yeast with alkali and a sodium chloride solution, carrying out saponifying, performing extracting by using methylbenzene, and successively carrying out washing, concentrating, refining and drying in vacuum to obtain a finished ergosterol product. According to the preparation method of the ergosterol, the yeast, the alkali, the sodium chloride, the methylbenzene, the activated carbon, the ethanol and other common chemical raw materials and auxiliary materials and common chemical equipment are utilized, industrial production of the ergosterol is achieved, a production process is very simple and convenient, and production cost is low; the volatilization speed of the methylbenzene is low and is only 1 / 3 of the original volatilization speed of the methylbenzene; and through reasonable selection of the ratio of each solution and temperature control, the purity and yield of the ergosterol are effectively improved, so a raw material guarantee is provided for mass production of vitamin D2.

The invention discloses high-adenosine and high-ergosterol fermented cordyceps sinensis fungus powder and a production method thereof, and relates to the technical field of fermented cordyceps sinensis fungus powder. The production method comprises the following steps: pumping fermentation broth that meets requirements into a raw material storage tank, and controlling tank pressure, material temperature and storage time; then filtering, pH change before and after filtering is less than or equal to 1.0, separating mycelium from the filtrate and removing fermentation broth; pumping into a concentration buffer storage tank, controlling storage vacuum degree, temperature and storage time, then concentrating, pH change before and after concentration is less than or equal to 1, and discharging; and putting the discharged material into a storage tank with a dry buffer after passing through an ultrahigh-temperature sterilizer at 135-180 DEG C for 4-7 seconds, controlling vacuum degree and storage time, and finally drying, crushing and the like to obtain a finished product. The method has beneficial effects that product quality is improved by controlling tank discharge standard, filtering, concentrating, drying and other process conditions, water content of the finished product is 1.0-7.0%, adenosine content is greater than or equal to 0.32%, and ergosterol content is greater than or equal to 0.4%.

The invention discloses ergosterol compounds with anti-inflammatory activity, and the ergosterol compounds are from Ganoderma sinensis and are as shown in a structural formula which is described in the specification. The invention also discloses a preparation method for the compounds and application of the compounds in pharmacy. The compounds can be applied as NO inhibitors to the preparation of anti-inflammatory drugs.

The invention discloses a synthetic method of ergosterol and derivatives thereof, which comprises the following steps: by taking a compound V as a raw material, carrying out side chain formylation and wittig reaction to obtain ergosterol and derivatives thereof. The structure formula of the compound V is shown in the specification. The invention provides the novel preparation method of ergosterol and derivatives thereof, the ergosterol is obtained in a synthesis manner, a target structure can be obtained in a targeted manner, the yield is high, and the purity is high. In addition, the synthetic route is short and environment-friendly, and is suitable for industrial production.

The present invention discloses a floor glue, which is made from the following raw materials by weight: 3.2-5.8 parts of water-based silicone oil, 3.2-4.7 parts of ergosterol, 5-10 parts of starch, 3.2-4.6 parts of oxhide gelatin, 3-7 parts of enzymolysis lignin, 0.8-2.7 parts of a polyamide epichlorohydrin resin, and 1-1.8 parts of acetyl tributyl citrate. According to the present invention, compared with the existing product in the prior art, the floor glue of the present invention has characteristics of long service life, high strength after drying, no shrinking, and no cracking; the product of the present invention has functions of sound absorbing and noise reducing, and further contains the oxhide gelatin and the enzymolysis lignin, such that the product of the present invention further has characteristics of fire prevention, air permeability, temperature adjusting and humidity adjusting; the product of the present invention has the high performance-price-ratio, and the cost is saved by 30% compared with other materials achieving the same market price, such that the product of the present invention has the substantial market competitiveness; and with application of the product of the present invention to construct, the process is simple and convenient, and 20% of the manpower is saved.

The invention provides an extraction method of an ergosterol monomer compound in Armillarialuteo-virens. The method includes: taking an Armillarialuteo-virens fruit body as the raw material, grinding the raw material into powder, then conducting extraction with ethyl acetate, and carrying out concentration into paste; conducting dissolving with a n-hexane-ethanol solvent; conducting one-dimensional liquid chromatography separation: employing a Silica chromatographic column, adopting a binary mobile phase with n-hexane as the phase A and ethanol as the phase B, performing gradient elution with phase B at a concentration ranging from 0% to 75%, and collecting the 8-11min eluent; performing dissolving with an ethanol-n-hexane solvent; carrying out two-dimensional liquid chromatography separation: adopting a Silica chromatographic column, employing a binary mobile phase with n-hexane as the phase A and ethanol as the phase B, performing isocratic elution with phase B at a concentration of 2-6%, collecting the 12-15min eluent, and performing evaporation concentration to obtain ergosterol. The separation method has the advantages of stability, high repeatability, large preparation capacity, simple operation and time saving, low cost, and high ergosterol extract rate. The target object has an inhibiting effect on lung cancer A549 cells and liver cancer Hep-G2 cells, and has anticancer drug development potential.

The invention discloses a calcium tablet containing ergosterol and a preparation method thereof. The preparation method comprises the following steps: carrying out aerobic fermentation cultivation to saccharomyces cerevisiae by using a optimized culture medium, and improving the expression quantity of the saccharomyces cerevisiae to the ergosterol; and executing the solid-liquid separation to yeast fermentation liquor, freezing and drying the obtained supernate, to obtain a substance I; after diluting the obtained thallus by using normal saline, executing the solid-liquid separation through the pulsed electric field action, freezing and drying the obtained supernate, to obtain a substance II; mixing the substance I and the substance II, to obtain a substance A; uniformly mixing the substance A, Allah milk calcium, whole milk powder, maltodextrin and a sugarcane extractive, to obtain a mixture; and dissolving the mixture by using the water, spray-drying, pelletizing, drying, size-stabilizing, and tabletting, to obtain the calcium tablet containing the ergosterol. The provided calcium tablet containing the ergosterol is not only good for the absorption of the calcium, but also capable of effectively strengthening the human body immunity.