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Fasciation naematoloma ligarine extraction, preparing method thereof and use in preparing pharmaceutical for treating tumour

A technology for extracting petroleum ether and tumor drugs, which is applied in the field of preparation of tumor drugs and the extraction and preparation of active ingredients of traditional Chinese medicines. It can solve the problems of little research on clustered algae, no provision of extraction technology and determination of pharmacological activity, etc.

Inactive Publication Date: 2007-08-08
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After reviewing the domestic and foreign literature, there are few studies on the clustered algae, especially in the aspect of anti-tumor medicine, and there is no confirmation of its extraction process and pharmacological activity.

Method used

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  • Fasciation naematoloma ligarine extraction, preparing method thereof and use in preparing pharmaceutical for treating tumour
  • Fasciation naematoloma ligarine extraction, preparing method thereof and use in preparing pharmaceutical for treating tumour
  • Fasciation naematoloma ligarine extraction, preparing method thereof and use in preparing pharmaceutical for treating tumour

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Dried 1kg of the fruiting bodies of the clustered umbrella at 40°C, crushed them, and leached them with hot methanol at 50°C. The amount of methanol was 1.5 times the volume of the drug powder. Repeated extraction twice, combined with 2500mL of methanol extracts, concentrated to 250mL, add 5 times of distilled water to form a suspension, add 1 times of petroleum ether for extraction three times, combine 3500mL of petroleum ether extracts, concentrate, evaporate the petroleum ether solvent in a water bath, and obtain 4.3g of petroleum ether extract. Its GC analysis spectrum is shown in Figure 1.

Embodiment 2

[0085] Put 3.0 g of the petroleum ether extract of the chrysanthemum fruticosa obtained in Example 1 on a silica gel column for separation. Wherein, the silica gel column is 200-300 mesh silica gel, packed by wet method; the elution solvent is cyclohexane:ethyl acetate=7:5. Available monomeric compounds ergosterol peroxide 230mg and ergosterol 14mg; melting points are 177-179°C and 152-155°C respectively.

[0086] The melting point of the obtained monomeric compound ergosterol peroxide is 177-179°C, white needle crystal; melting point: 178°C; solubility: easily soluble in petroleum ether, chloroform, ethyl acetate, acetone, slightly soluble in ethanol, methanol, Insoluble in water, acetic anhydride and concentrated sulfuric acid react positively, and its spectrum is shown in attached drawings 2-9.

[0087] The melting point of the monomer compound ergosterol is 152-155°C, white needle crystal; solubility: easily soluble in petroleum ether, chloroform, ethyl acetate, acetone, ...

Embodiment 3

[0089] Dried 1 kg of the fruiting bodies of the clustered Umberella at 30°C, crushed, extracted with hot methanol at 60°C, extracted three times with 2 times the amount of methanol, combined the methanol extract 3000mL, concentrated 300mL, and added 5 times distilled water to form a suspension solution, add 1 times the ratio of petroleum ether for extraction three times, combine 5000mL of petroleum ether extracts, concentrate, evaporate the petroleum ether in a water bath, and obtain 5.6g of the petroleum ether extract of the clustered cymbidium. Its GC analysis spectrum is shown in Figure 1.

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Abstract

The invention discloses a cluster naematolin ligarine extract, which separates ergosterin peroxidate and two monomer compounds of ergosterin. The invention also provides making method of extract from cluster naematolin fruiting body and mycelium for industrial manufacturing, which is fit for treat tumour drug.

Description

Technical field: [0001] The invention discloses a kind of petroleum ether extract of the clustered silk umbrella, and further separates the two main monomeric compounds ergosterol peroxide and ergosterol, and provides their application in the preparation of drugs for treating tumors, which belongs to the traditional Chinese medicine effective Component extraction preparation method and medical application technical field. Background technique: [0002] The so-called Naematoloma fasciculare in the present invention, also known as: cluster yellow mushroom, poison tough black umbrella, cluster yellow tough umbrella, is bitter in taste, and is a kind of poisonous mushroom that can kill people after eating. After reviewing the domestic and foreign literature, there are few studies on the clustered algae, especially in the aspect of anti-tumor medicine, and there is no confirmation of its extraction process and pharmacological activity. Invention content: [0003] The invention...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00C07J9/00A61K31/58A61K31/575A61K36/07A61P35/00
Inventor 包海鹰丁燕图力古尔李玉
Owner JILIN AGRICULTURAL UNIV
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