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Method for promoting germination of bletilla striata seeds

A technology of seeds and bletilla striata, applied in the field of promoting the germination of bletilla striata seeds, can solve the problems of difficult seed clustering, troublesome subculture, long cultivation time, etc., to improve the efficiency of subculture and seed germination rate, facilitate the separation of clusters, and the germination speed accelerated effect

Inactive Publication Date: 2017-06-20
ANHUI ZHENYANG AGRI & FORESTRY ECOLOGY DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The current cultivation methods are relatively traditional cultivation methods, which are prone to long cultivation time, difficult separation of clustered seeds, troublesome subculture, low germination rate, etc., which are not conducive to seed germination.

Method used

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Examples

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Effect test

Embodiment 1

[0018] A method for promoting germination of bletilla striata seeds, comprising the following steps:

[0019] Collect the uncracked capsules 15 weeks after the flowering and pollination of the Baiji plant, wash them with running water, then disinfect the surface with 75% alcohol for 1 minute, then disinfect them in 0.1% solution for 10 minutes, and rinse them with sterile water for 4 to 6 minutes. The second time, use sterile absorbent paper to suck up the remaining liquid and prepare it for later use. In the laboratory, prepare the chemical components required to configure 1 / 2 MS medium to make a mother solution, in which macroelements, trace elements, inositol, and vitamins are separated in the form of mother solution. Preserve, mix and use the iron salt now;

[0020] When preparing the culture medium, weigh 100L of the mother solution required for the 1 / 2MS medium in the laboratory, first add some purified water to the preparation container to dissolve the sucrose completel...

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Abstract

The invention discloses a method for promoting germination of bletilla striata seeds, and relates to the field of orchid cultivation. The method comprises the following steps: preparing a culture medium, carrying out sterilization at states of 121 DEG C and 0.1 Kpa for 20 minutes, collecting non-cracked capsules at the 15th week after a bletilla striata plant is bloomed and pollinated, cleaning and disinfecting the capsules, then longitudinally cutting off the capsules, slightly shaking off powdered seeds to the surface of the culture medium, and starting to carry out cultivation under conditions that the temperature is (25+ / -1) DEG C, the illumination intensity is 2,000 to 2,500 lx and the illumination time is 16 hours per day. The method is simple and easy to use, and can promote increase of the germination speed of the bletilla striata seeds for bletilla striata and shorten the cultivation time; fasciation separation is facilitated, the subculture efficiency is improved, and the seed germination rate is increased.

Description

[0001] Technical field: [0002] The invention relates to the field of orchid plant cultivation, in particular to a method for promoting the germination of bletilla striata seeds. [0003] Background technique: [0004] Baiji is a perennial herbaceous plant of the genus Baiji in the family Orchidaceae. Its flowers are large and colorful, and there are thick and fleshy medicinal massive pseudobulbs, which have high ornamental value and medicinal value. Baiji is mainly propagated by ramets, but its reproductive efficiency is low, and it is difficult to adapt to the needs of large-scale planting. For Orchidaceae plants, including white genus, a large number of seedlings can be obtained in a short period of time through aseptic sowing and tissue culture, which is a fast and effective propagation method. [0005] However, the current cultivation methods are relatively traditional cultivation methods, which are prone to long cultivation time, difficult separation of clustered seeds,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 刘沁洪陈金印殷道鋆苗桂林李为新张振李明祥王云
Owner ANHUI ZHENYANG AGRI & FORESTRY ECOLOGY DEV
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