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Wine brewing yeast strain and method for producing ethanol by efficient stalk fermentation

A Saccharomyces cerevisiae strain and technology for fermenting straw, which are applied in the directions of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve problems such as increasing costs, increasing the difficulty of separation, and destroying resistance, and achieves improved production efficiency and shortened time. Fermentation time, effect on improving ethanol yield and yield

Inactive Publication Date: 2009-05-20
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main pretreatment methods are high temperature dilute acid pretreatment and steam explosion technology. Pretreatment can destroy the resistance of raw materials to the enzymatic hydrolysis process, but also produces a large number of inhibitors, thereby inhibiting the growth and development of yeast in the hydrolyzate. ethanol production
In order to effectively ferment cellulose hydrolyzate, researchers have proposed many methods to remove inhibitors in hydrolyzate to improve fermentation efficiency, including alkali neutralization, ion exchange, solvent extraction and other methods, but these not only increase the cost , which also increases the difficulty of subsequent separation

Method used

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  • Wine brewing yeast strain and method for producing ethanol by efficient stalk fermentation
  • Wine brewing yeast strain and method for producing ethanol by efficient stalk fermentation

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Experimental program
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Effect test

Embodiment 1

[0043] Please see attached figure 2 , is the fermentation cycle curve, steam-exploded straw is the raw material, the initial solid content of the fermenter is 15%, cellulase 15FPU / DM is added, enzymolysis is performed at 50°C for 12 hours, and the seed liquid cultivated according to the two steps is inserted into the fermenter at 10% In the following feeding operation; the calcium hydroxide saturated solution adjusts the pH to 5.0, and the nutrient concentration is respectively KH 2 (PO 4 ) 3 2g / L, MgSO 4 1g / L, (NH 4 ) 2 SO 4 2g / L, yeast extract powder 1g / L, so that the solid content reaches 25%, the fermentation period is controlled at 72 hours, the ethanol concentration reaches 6.0%, and the yield is 78%.

Embodiment 2

[0045] Corn stalks were pretreated at 180°C with 5% sulfuric acid concentration for 5 minutes to obtain the pretreatment liquid and pretreated stalks, then the stalks were pretreated with dilute acid to adjust the liquid-solid ratio and hydrolyzed with enzyme 15FPU / DM for 48 hours, and centrifuged to obtain the supernatant. Use solid calcium hydroxide to adjust the pH; steam-explode the straw to adjust the water content, and also add enzyme 15FPU / DM to hydrolyze for 48 hours, and obtain the supernatant after centrifugation. According to the two-stage culture method, the seeds were obtained, and 10% was inoculated in the centrifuged hydrolyzate, and the nutrient concentrations of seed culture and fermentation were respectively KH 2 (PO 4 ) 3 3g / L, MgSO 4 2g / L, (NH 4 ) 2 SO 4 3g / L, yeast extract powder 2g / L, fermented at 37°C for 72 hours, the yield of ethanol based on glucose was 89%.

Embodiment 3

[0047] Corn stalks were pretreated at 200°C with 0.5% sulfuric acid concentration for 1 min to obtain pretreatment liquid and pretreated stalks, then dilute acid pretreated stalks to adjust liquid-solid ratio and hydrolyze with enzyme 5FPU / DM for 48 hours, and centrifuged to obtain supernatant; The steam-exploded straw was adjusted to the water content, and the enzymatic hydrolysis 5FPU / DM was added to saccharify for 48 hours, and the supernatant was obtained after centrifugation. According to the two-stage culture method, the seeds were obtained, and 10% was inoculated in the centrifuged hydrolyzate, and the nutrient concentrations of seed culture and fermentation were respectively KH 2 (PO 4 ) 3 3g / L, MgSO 4 2g / L, (NH 4 ) 2 SO 4 3g / L, yeast extract powder 2g / L, fermented at 37°C for 72 hours, the yield of ethanol based on glucose was 85%.

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Abstract

The invention discloses a Saccharomyces cerevisiae strain and a method for producing ethanol by fermenting stalks, the strain is Saccharomyces cerevisiae DQ1, the collection No. is CGMCC No.2528, the strain can tolerate methanoic acid, acetic acid and furfural type inhibitors in hydrolyzate of the stalks, tolerate high glucose concentration and ethanol concentration and have wide growth temperature, and the strain is fermented at high temperature; the method for producing the ethanol by fermenting the stalks comprises two-stage culture of seeds, wherein the first stage culture is carried out in a synthetic culture medium for 16-24 hours, the second stage culture is that a mixed culture medium containing 30-70 percent of hydrolyzate is inoculated into with the inoculation amount of 5-10 percent for culture for 15-20 hours, and the inoculation into the hydrolyzate with the inoculation amount of 10 percent or simultaneous saccharification and fermentation of the stalks is further carried out for producing the ethanol. The invention has the advantages of being applicable to high-solid-content stalk enzyme hydrolysis and simultaneous saccharification and fermentation, with short fermentation period, high ethanol yield, and low production cost, thereby having great industrial application prospect.

Description

【Technical field】 [0001] The invention relates to the technical field of biological fermentation, in particular to a method for producing ethanol by Saccharomyces cerevisiae strain Saccharomyces cerevisiae DQ1CGMCC No.2528. 【Background technique】 [0002] Energy issues are related to important fields such as people's lives, economic development, environmental protection and national security, and have always been the focus of attention and research by countries all over the world. With the dwindling reserves of fossil fuels, global warming, and people's demand for energy, it is imminent to seek renewable energy. Bioethanol can partially replace gasoline while reducing greenhouse gas emissions, and is becoming a liquid alternative fuel of great interest. [0003] At present, the main fermentation raw materials for ethanol are starchy raw materials such as corn, which has caused a rise in food prices and led to a situation where people and vehicles compete for food. In the lo...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12P7/10C12R1/865
CPCY02E50/16Y02E50/10
Inventor 鲍杰楚德强张建
Owner EAST CHINA UNIV OF SCI & TECH
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