Culture medium composition for culturing chlorella
A composition and medium technology, applied in the direction of microorganism-based methods, microorganisms, single-cell algae, etc., can solve the problems of low absorption and utilization efficiency, decline in algal cell quality, low protein and fatty acid content, etc., to reduce cultivation costs, Promotes oil accumulation and increases oil content
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Embodiment 1
[0035] Add 100mL of the medium shown below in a 250mL shake flask, the inoculation ratio is 10% (that is, the volume of the seed solution accounts for 10% of the culture volume), wherein the seeds are domesticated Chlorella, heterotrophic culture (filling volume 100mL , temperature 30 degrees, shaking table speed 100rpm) for 48 hours, the result showed that the maximum cell density was 14.24g / L, and the oil content was 30.8% (the total oil content in the cell accounts for the percentage of the total dry cell weight).
[0036] Per liter medium composition:
[0037] Glucose 15g Na 2 SO 3 0.040g
[0038] KNO 3 0.35g trace element mother liquor 0.5mL
[0039] K 2 HPO 4 ·3H 2 O 0.075g MgSO 4 ·7H 2 O 0.075g
[0040] CaCl 2 2H 2 O 0.025g KH 2 PO 4 0.175g
[0041] NaCl 0.025 Soil extract (soil extract) 30mL
[0042] FeCl 3 ·6H 2 O 0.005g Fe-EDTA 1mL
[0043] Distilled water balance
[0044] Among them, the formula of 1 liter trace element mother solution: H 3 B...
Embodiment 2
[0049] Add 100mL of the medium shown below in a 250mL shake flask, and the inoculation ratio is 10% (that is, the volume of the seed solution accounts for 10% of the culture volume). Amount of 100mL, temperature 30 degrees, shaking table speed 100rpm) 48 hours, the maximum cell density measured as a result was 19.41g / L, and the oil content was 35.2% (the total oil content in the cell accounts for the percentage of the total cell dry weight).
[0050] The fungus residue treatment solution is obtained by hydrolyzing the bacteria or yeast residue. Among them, Klebsiella pneumoniae is preferred, which uses glycerol as the only substrate to ferment and produce 1,3-propanediol under anaerobic conditions. After fermentation, after cooking at 80°C for 40 minutes, filter the filter cake of bacterial residue at room temperature, dry the filter cake of bacterial residue, and then hydrolyze it in 0.1mol / L NaOH solution. The solid-to-liquid ratio of the hydrolysis process is 1:20, the tre...
Embodiment 3
[0065] Add 100mL of the medium shown below to a 250mL shake flask, and the inoculation ratio is 10% (that is, the volume of the seed liquid accounts for 10% of the culture volume). The seeds used are from Chlorella vulgaris, which has not been cultured heterotrophically process, directly to its heterotrophic culture (filling volume 100mL, temperature 30 degrees, shaker rotation speed 100rpm) 48 hours, the result records that the maximum cell density is 11.30g / L, and the oil content is 28.6% (the total oil content in the cell accounts for percentage of total cell dry weight).
[0066] The composition of each liter medium is as follows:
[0067] Glucose 30g Na 2 SO 3 0.020g
[0068] KNO 3 0.25g trace element mother liquor 0.1mL
[0069] K 2 HPO 4 ·3H 2 O 0.075g MgSO 4 ·7H 2 O 0.075g
[0070] CaCl 2 2H 2 O 0.025g KH 2 PO 4 0.175g
[0071] NaCl 0.025 Soil extract (soil extract) 30mL
[0072] FeCl 3 ·6H 2 O 0.005g Fe-EDTA 1mL
[0073] Distilled water balance ...
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