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Microalgae suspension-adhesion mixed culture and separated harvesting method based on suspended carrier

A technology of mixed culture and harvesting method, which is applied in the field of production and harvesting of microalgae and related products, can solve the problems of complex operation, uneconomical chemicals, unstable fixation of longitudinal algae film, and restrictions on large-scale application, so as to improve the resistance The effect of environmental interference ability, saving the cost of using medicine, and easy operation

Inactive Publication Date: 2013-11-27
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some fixing materials have a toxic effect on the growth of algae cells. At the same time, the complexity of operation and the uneconomical use of chemicals restrict the development of this method.
[0005] In addition, the existing plate-attached culture of microalgae can solve the problem of difficult harvesting of algae. However, the operation of supplying nutrients and carbon dioxide is complicated. With the large-scale application of this method

Method used

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  • Microalgae suspension-adhesion mixed culture and separated harvesting method based on suspended carrier
  • Microalgae suspension-adhesion mixed culture and separated harvesting method based on suspended carrier
  • Microalgae suspension-adhesion mixed culture and separated harvesting method based on suspended carrier

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Embodiment 1

[0031] Such as figure 2 As shown, the microalgae are cultivated in a cylindrical suspended carrier photobioreactor, and the suspended carrier and the algae cells have a certain relative movement by aeration at the bottom. Inoculate Scenedesmus S.LX1 into the prepared mBG11, the initial concentration is about 1×10 4 pcs / ml, with a total volume of 4L, add 6 suspension carriers (each suspension carrier is bundled by 100×15cm fiber materials) and place them in a photobiological reaction column for aerated culture, where the light-dark ratio is 14:10, and the light Strong 800~1300Lux, temperature is room temperature. The algal cell attachment on the carrier changes as image 3 As shown, after 2 days of culture, the solid phase algae film first appeared, about 2.0×10 5 pcs / cm carrier. After 10 days of culture, the algae density in the liquid phase can reach 3.4×10 6 cells / mL, the solid-phase algae cell density can reach 4.2×10 6 pcs / cm carrier.

Embodiment 2

[0033] Such as Figure 4 As shown, microalgae are cultivated in a cuboid fully aerated suspension-type carrier photobioreactor, and the volume of the liquid phase in the reactor is about 8L. In this embodiment, the bottom side aeration method (air or carbon dioxide-containing air) is adopted to make the suspended microalgae and the carrier flow in the reactor. Microalgae are attached to the surface of the carrier, and with the circulation of the fluid, they are periodically exposed to sunlight for photosynthesis. During the aeration process, the motility of algal cells is increased, that is, the probability of algal cells being captured and attached to the carrier is increased. The algal cells on the carrier can use the carbon dioxide supplemented during the aeration process to achieve regrowth after attachment, making the carrier The thickness of the algae film on the surface increases, which is convenient for harvesting. Formed algae film under the optical microscope metho...

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Abstract

A microalgae suspension-adhesion mixed culture and separated harvesting method based on a suspended carrier comprises the following steps: on the basis of original microalgae suspension culture, a predetermined amount of solid carrier is added into a culture solution and enabled to suspend in the liquid phase in an aerating or mechanical stirring manner; during cultivation, the microalgae is enabled to attach to a solid carrier to form an algae membrane; the solid carrier is separated to harvest algae cells; the solid carrier from which the algae cells are harvested is recovered for recycling and re-injected into the microalgae culture solution; serving as seeds, the liquid-phase algae cells are re-attached to the solid carrier to grow, so that continuous culture and harvest of the algae cells are realized. Compared with the air floatation, precipitation, centrifugation, filtering and other harvest methods, the microalgae suspension-adhesion mixed culture and separated harvesting method is simple to operate, dispenses with agents, is low in energy consumption, and can realize large-scale and mechanical continuous harvesting, thereby effectively saving the cost in harvesting the algae cells.

Description

technical field [0001] The invention relates to the field of production and harvesting of microalgae and related products, in particular to a microalgae suspension-attachment mixed culture and separation and harvesting method based on a suspension carrier. Background technique [0002] In today's society, the energy crisis is severe, and microalgae biomass energy has attracted widespread attention as a new energy source. Microalgae have the characteristics of short growth cycle, clean and pollution-free, and have the characteristics of carbon sequestration. [0003] A major factor restricting the large-scale development of microalgae biomass energy is the problem of microalgae harvesting. For the traditional cell suspension culture of microalgae, the general harvesting methods are air flotation, sedimentation, centrifugation, filtration, extraction, etc. Although these methods are effective, they are costly and economical, which restricts the large-scale market application...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 胡洪营庄林岚巫寅虎张天元朱树峰
Owner TSINGHUA UNIV
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