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Direct vat set clostridium butyricum starter and preparation method thereof

A technology of Clostridium butyricum and starter, applied in the direction of biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve problems that have not been reported, and achieve control of production process, stable product quality, and high content of live bacteria Effect

Active Publication Date: 2012-02-01
SHENZHEN JINXINNONG FEED
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the application of direct throwing Clostridium butyricum starter at home and abroad

Method used

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  • Direct vat set clostridium butyricum starter and preparation method thereof
  • Direct vat set clostridium butyricum starter and preparation method thereof
  • Direct vat set clostridium butyricum starter and preparation method thereof

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Embodiment Construction

[0026] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.

[0027] A Clostridium butyricum TK2, taxonomic name Clostridium butyricum, preservation number: CGMCC No.4729, preservation date: April 2, 2011, preservation address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, preservation Unit: General Microbiology Center of China Committee for Culture Collection of Microorganisms. The following descriptions are based on this strain.

[0028] 1. Seed and fermentation medium:

[0029] Liquid seeds and fermentation medium (%): peptone 1.75-2; glucose 0.5, MgSO 4 0.02,K 2 HPO 4 0.5, MnSO 4 0.02, pH7.4.

[0030] 2. Seed preparation of bacteria:

[0031] The strains frozen in glycerol tubes were transferred to test tubes containing liquid seed medium, and incubated at 37°C for 16 h...

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Abstract

The invention relates to a direct vat set clostridium butyricum starter and a preparation method thereof. The method comprises the following steps of: performing clostridium butyricum seed culture; performing high-density scale-up culture; preparing thallus starter by using a freeze-drying method; and finally obtaining the direct vat set clostridium butyricum starter, wherein one liter of culturemedium for clostridium butyricum seed culture and high-density scale-up culture is prepared from 1.75-2g of peptone, 0.5g of glucose, 0.02g of MgSO4, 0.5g of K2HPO4 and 0.02g of MnSO4; and concentration conditions for preparing the thallus starter by using the freeze-drying method are that: the centrifugal rotation speed is 5,000r / min, centrifugation time is 10 minutes, centrifugation temperatureis 4 DEG C, and a freeze-dry protecting agent comprises 10 percent dry skim milk and 10 percent malt extract. The survival rate of the direct vat set clostridium butyricum starter produced by the method is 94.6 percent, the viable count is 3.92*10<10>cfu / g, and an ideal liquid-state and solid-state fermentation effect can be achieved according to inoculum size of 0.1 percent. A novel preparation method for the high-activity direct vat set clostridium butyricum starter is developed on the basis of the strain to be applied to fermentative culture of clostridium butyricum.

Description

technical field [0001] The invention belongs to the field of biological preparations, in particular to a direct-throwing Clostridium butyricum starter and a preparation method thereof. Background technique [0002] Clostridium butyricum is a kind of anaerobic bacilli with endophytic spore structure. It is a normal microorganism beneficial to the host. The preparation made by special technology can inhibit the growth of harmful bacteria through the competitive exclusion of organisms in the digestive tract of animals. , forming dominant flora or preventing diseases by enhancing non-specific immune function, can achieve the purpose of adjusting the micro-ecological balance in the body, thereby promoting animal growth and improving feed conversion rate, reducing the odor of feces. In the context of the increasingly prominent disadvantages of using antibiotics, the many advantages of live Clostridium butyricum preparations make it of great significance in the prevention and treat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16C12N1/20C12R1/145A23K10/18
Inventor 王海宽刘菲菲戚薇李安东
Owner SHENZHEN JINXINNONG FEED
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