Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for preparing 7α, 15α-dihydroxydhydroepiandrosterone by using Trichospora flaxensis

A technology of Trichosporium flaxensis and dehydroepiandrosterone, applied in the field of hydroxylation, can solve problems such as poor effect, difficult product separation, and expansion of production scale

Active Publication Date: 2019-01-11
ZHEJIANG XIANJU JUNYE PHARM CO LTD
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chinese patent CN102703558B discloses a method of converting DHEA into 7α-hydroxyl-dehydroepiandrosterone and 7α, 15α-dihydroxydehydroepiandrosterone by using Colletotrichum lini ST-1 (preservation number is CGMCC No.6051). Hydroepiandrosterone and the conversion method adopt the method of pre-inclusion of the substrate and the methyl-β-cyclodextrin of equimolar ratio before feeding, and the formation can improve the solubility and conversion efficiency of the substrate. After our Experimental method, this method has high selectivity for strains, that is, when different strains deal with the substrate pre-included with methyl-β-cyclodextrin, the results are not consistent. It does not work well with methyl-β-cyclodextrin pre-inclusion substrates, and the product obtained by this method is a mixture of 7α-hydroxydehydroepiandrosterone and 7α, 15α-dihydroxydhydroepiandrosterone , according to the published data of the patent, only 60% of the conversion products are 7α, 15α-dihydroxydhydroepiandrosterone, and the separation of these two products is relatively difficult
[0004] Chinese patent CN 102757997A discloses a method for preparing 7α, 15α-dihydroxyandrostenone (i.e. 7α, 15α-dihydroxydhydroepiandrosterone) by a substrate-inducing method, adding substrates in stages to make the substrate The final addition concentration of the method reaches 8-10g / L, and can increase the conversion rate to about 90%. However, through our experiments, we found that this method needs to prolong the total fermentation time, thereby affecting production efficiency, and the inoculum size used in this method 8-10%, larger inoculation volume will actually restrict the expansion of production scale

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for preparing 7α, 15α-dihydroxydhydroepiandrosterone by using Trichospora flaxensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] Below in conjunction with the examples, the specific implementation of the present invention will be further described in detail. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0023] Strain: Colletotrichum lini ST-1, preservation number CGMCC No.6051

[0024] Substrate raw material: dehydroepiandrosterone (DHEA), content 98%,

[0025] Fumaryl chitosan, p-hydroxybenzoyl chitosan, and benzoyl chitosan are all according to Reference 1—Study on the chemical modification of chitosan and the antibacterial activity of its derivatives (Feng Yongwei, Jiangnan University Doctoral dissertation, June 2011), prepared by the disclosed method, wherein the degree of substitution of carboxymethyl oleoyl chitosan is 0.45-0.48, the degree of substitution of benzoyl chitosan is 1.2-1.3, and the degree of substitution of p-hydroxybenzoyl chitosan is 0.45-0.48. The degree of substitution of acyl chit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
degree of substitutionaaaaaaaaaa
degree of substitutionaaaaaaaaaa
degree of substitutionaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for preparing 7alpha,15alpha-dyhydroxyldehydroepiandrosterone by utilizing flax colletotrichum mould. The method comprises the following steps: (1) performing spore culture, namely taking flax colletotrichum mould ST-1 as a production strain, performing slant culture, thereby obtaining mature spores; (2) performing mycelium culture, namely performing mycelium culture in a mycelium culture medium in which the spores obtained in the step (1) are inoculated, thereby obtaining the mycelium culture liquid; (3) performing biotransformation, namely adding carboxymethyl oleoyl-chitosan and DHEA into the mycelium culture liquid in the step (1), wherein the final concentration of the DHEA is 10.5-12g / L, the weight ratio of the carboxymethyl oleoyl-chitosan to DHEA is (2-3):1, transforming under the stirring and rotating speed of 200-220 revolutions per minute for 36-60 hours, thereby obtaining the transformation liquid; and (4) extracting the product, extracting and concentrating the transformation liquid by using ethyl acetate, thereby obtaining the 7alpha,15alpha-dyhydroxyldehydroepiandrosterone.

Description

technical field [0001] The steroidal compound biotransformation method of the present invention specifically designs a hydroxylation method. Background technique [0002] Drospirenone is a new type of progestin, because it has a certain mineralocorticoid effect, when it is used to prepare oral short-acting contraceptives, it can overcome the side effect of weight gain caused by general progesterone. 7α, 15α-dihydroxy dehydroepiandrosterone (ie 7α, 15α-dihydroxy dehydroepiandrosterone, referred to as 7α, 15α-diOH-DHEA, CAS: 2963-69-1) is an important Intermediates are generally produced by biotransformation or dehydroepiandrosterone (DHEA) due to the difficulty of synthesis. [0003] Chinese patent CN102703558B discloses a method of converting DHEA into 7α-hydroxyl-dehydroepiandrosterone and 7α, 15α-dihydroxydehydroepiandrosterone by using Colletotrichum lini ST-1 (preservation number is CGMCC No.6051). Hydroepiandrosterone and the conversion method adopt the method of pre-...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/06C12R1/645
Inventor 张峥斌张汝金尹金玉高成华
Owner ZHEJIANG XIANJU JUNYE PHARM CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products